Multidrug resistance in Shiga toxin-producing Escherichia coli (STEC) isolated from broiler chickens at slaughter

Rodrigo Pacheco Ornellas, Hugo Peralva Lopes, Daniela de Queiroz Baptista, Thomas Salles Dias, Arthur de Almeida Figueira, Gisllany Alves Costa, Leandro dos Santos Machado, Nathalie Costa da Cunha, Virginia Léo de Almeida Pereira, Dayse Lima da Costa Abreu

Abstract


Broiler chickens and derived products are a key source of Shiga toxin-producing Escherichia coli (STEC) in humans. This pathotype is responsible for causing severe episodes of diarrhea, which can progress to systemic complications. A rapid and accurate diagnosis of the disease, and early treatment of the infection with antimicrobials, can prevent it worsening. However, multidrug-resistant strains have potentially negative implications for treatment success. In this context, the aim of the present study was to isolate and identify multidrug-resistant STEC strains from broiler chickens and carcasses. Of 171 E. coli strains, isolated by conventional microbiological techniques and submitted to Polymerase Chain Reaction (PCR), for detection of stx1 and stx2 genes, 21.05% (36/171) were STEC pathotype, and most of them (66.67% - 24/36) carried both stx1 and eae genes. The multidrug resistance pattern was observed in 75% (27/36) of STEC strains. The presence of STEC in broiler chickens and carcasses reinforces that these sources may act as reservoirs for this pathotype. Multidrug-resistant bacteria contaminating animal products represent a public health issue because of the possibility of spread of multidrug-resistant determinants in the food chain and a higher risk of failure in human treatment when antimicrobials are needed.

Keywords


Broiler carcasses; Escherichia coli; Multidrug-resistance; STEC.

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References


Alikhani, M. Y., Hashemi, S. H., Aslani, M. M., & Farajnia, S. (2013). Prevalence and antibiotic resistance patterns of diarrheagenic Escherichia coli isolated from adolescents and adults in Hamedan, Western Iran. Iranian Journal of Microbiology, 5(1), 42-47. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC3577554/

Alonso, M. Z., Lucchesi, P. M. A., Rodríguez, E. M., Parma, A. E., & Padola, N. L. (2012). Enteropathogenic ( EPEC ) and shigatoxigenic Escherichia coli (STEC) in broiler chickens and derived products at different retail stores. Food Control, 23(2), 351-355. doi: 10.1016/j.foodcont.2011.07.030

Andreatti, R. L., Fº., Gonçalves, G. A. M., Okamoto, A. S., & Lima, E. T. de. (2011). Comparação de métodos para extração de DNA na reação em cadeia da polimerase para detecção de Salmonella enterica sorovar Enteritidis em produtos avícolas. Ciência Animal Brasileira, 12(1), 115-119. doi: 10.5216/cab. v12i1.3774

Barros, M. R., Silveira, W. D. da, Araújo, J. M. de, Costa, E. P., Oliveira, A. A. da F., Santos, A. P. da S. F.,... Mota, R. A. (2012). Resistência antimicrobiana e perfil plasmidial de Escherichia coli isolada de frangos de corte e poedeiras comerciais no Estado de Pernambuco. Pesquisa Veterinária Brasileira, 32(5), 405-410. doi: 10.1590/S0100-736X2012000500008

Blaak, H., Hoek, A. H. A. M. Van, Hamidjaja, R. A., Plaats, R. Q. J. Van Der, Heer, L. K., Maria, A.,... Schets, F. M. (2015). Distribution , numbers , and diversity of ESBL- producing E . coli in the poultry farm environment. Plos One, 8(13), 1-23. doi: 10.1371/journal.pone.0135402

Brasil, M. da S. (2019). Surtos de doenças transmitidas por alimentos no Brasil. Brasília: Secretaria de Vigilância em Saúde. Retrieved from https://portalarquivos2.saude.gov.br/images/pdf/2019/fevereiro /15/Apresenta----o-Surtos-DTA---Fevereiro-2019.pdf

Clinical and Laboratory Standards Institute (2018). Performance standards for antimicrobial susceptibility testing, M100 performance standards for antimicrobial susceptibility testing (28nd ed.). Wayne: CLSI.

Doregiraee, F., Alebouyeh, M., Fasaei, B. N., Charkhkar, S., Tajedin, E., & Zali, M. R. (2016). Isolation of atypical enteropathogenic and shiga toxin encoding Escherichia coli strains from poultry in Tehran, Iran. Gastroenterology and Hepatology from Bed to Bench, 9(1), 53-57. doi: 10.22037/ghfbb.v1i9.866

El-Rami, F. E., Rahal, E. A., Sleiman, F. T., & Abdelnoor, A. M. (2012). Identification of virulence genes among antibacterial-resistant Escherichia coli isolated from poultry. Advanced Studies in Biology, 4(8), 385-396. Retrived from http://www.m-hikari.com/asb/asb2012/asb5-8-2012/abdelnoorASB5-8-2012. pdf

Ethelberg, S., Olsen, K. E. P., Scheutz, F., Jensen, C., Schiellerup, P., Engberg, J.,... Mølbak, K. (2004). Virulence factors for virulence factors for hemolytic uremic syndrome, Denmark. Emerging Infectious Diseases, 10(5), 843-847. doi: 10.3201/eid1005.030576

Fürst, S., Scheef, J., Bielaszewska, M., Rüssmann, H., Schmidt, H., & Karch, H. (2000). Identification and characterisation of Escherichia coli strains of O157 and non-O157 serogroups containing three distinct Shiga toxin genes. Journal of Medical Microbiology, 49(4), 383-386. doi: 10.1099/0022-1317-49-4-383




DOI: http://dx.doi.org/10.5433/1679-0359.2021v42n6SUPL2p3813

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433 / 1679-0359
E-mail:  semina.agrarias@uel.br
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