Comparative analysis of the hormone production and gene expression profiles in ovine uterus tissue during oestrus cycle synchronized using medroxyprogesterone acetate plus eCG and prostaglandin analogue

Joaquim de Sousa Lima, Márcio da Silva Costa, Júlio Rodrigues Pereira Júnior, Guilherme José Bolzani de Campos Ferreira, Luciana Rocha Faustino, Carlos Eduardo Fonseca Alves, Eunice Oba, Anna Clara Accioly Ferreira, José Ricardo de Figueiredo, Cleidson Manoel Gomes da Silva

Abstract


The combination of medroxyprogesterone acetate (MPA) and gonadotropin chorionic (eCG) has been widely used to synchronize oestrus cycle in sheep, but their effects on the gene expression in uterine tissue are yet to be elucidated. To evaluate the effect of MPA + eCG or prostaglandin analogue (PA) treatments on the rate of oestrus cycle synchronization, as well as further hormone production and gene expression profiles in uterine tissue, 14 Santa Inês ewes were randomly selected. The MPA + eCG group (n = 7) received intravaginal insertion of MPA-impregnated sponges for 14 days and was administered 350 IU eCG on the day of sponge withdrawal. The PA group (n = 7) was administered two doses of 100 ug of PA separated by 12 days. The ewes were assessed for the rate of oestrus cycle synchronization and the serum concentrations of progesterone (P4) and estradiol (E2). Additionally, the expression of estrogen receptor (ERalfa), progesterone receptor (P4R), and immunolocalization of interferon receptor (IFNAR1) in the uterine tissue samples collected 15 th day post-mating were examined. The rate of oestrus cycle synchronization was 100% (n=7/7) and 57.14% (n=4/7) in the MPA + eCG and PA groups, respectively. Moreover, the MPA + eCG group exhibited higher serum concentration of P4 than the PA group (p smaller 0.05). However, the E2 serum concentration did not differ between the two groups (p larger 0.05). The relative expression of P4R and ERalfa mRNA analyzed using real-time PCR and immunodetection of IFNAR1 were similar between the two groups tested (p larger 0.05). Conclusively, MPA + eCG treatment improved the rate of oestrus cycle synchronization and endogenous P4 production; however, it did not affect the expression of sex steroid receptors and IFNAR1 in uterine ovine tissue.

Keywords


Oestrus cycle; Progesterone receptor; Estrogen receptor-alfa; IFNAR1; Sheep.

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References


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DOI: http://dx.doi.org/10.5433/1679-0359.2021v42n6p3321

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional