Effect of the antral follicle count of Bos taurus × Bos indicus dairy cows on in vitro embryo production

Sheila Merlo Garcia, Paula Alvares Lunardelli, Kleber Luciano Ancioto, Eduardo Cardoso de Oliveira, Larissa Zamparone Bergamo, Amanda Fonseca Zangirolamo, Marcelo Marcondes Seneda


This study aimed to evaluate the potential of Girolando (Bos taurus × Bos indicus) cows with high and low antral follicle counts (AFC) for the in vitro production of bovine embryos (IVEP), as well as the pregnancy rates of the recipients of these embryos. Girolando cows (Bos taurus × Bos indicus) were classified as high and low AFC when they had 35-52 (n = 13) and 11-17 follicles (n = 15), respectively. All animals were subjected to repeated follicular aspiration [Ovum pick-up (OPU)] and subsequent IVEP sessions. The synchronization protocol of the recipients was performed on a random day of the estrous cycle (Day 0) with the implantation of progesterone, estradiol benzoate, and prostaglandin. The high AFC group had higher aspirated oocyte/OPU (42.6 ± 5.2 vs. 14.6 ± 1.9; p < 0.01) and cultured oocyte/OPU (38.1 ± 6.6 vs. 12.3 ± 2.8; p < 0.01) averages as well as a higher blastocyst percentage on D7 (23.0 ± 1.0% vs. 18.4 ± 1.5%; p < 0.05) and higher pregnancy rate (42.7 ± 2.7% vs. 39.7 ± 4.6%; p < 0.05) than the low AFC group. Thus, we can conclude that animals with high AFC had better IVEP success rates than animals with low AFC.


AFC; OPU.;Embryo production; Bos taurus × Bos indicus.

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Ireland, J. J., Ward, F., Jimenez-Krassel, F., Ireland, J. L. H., Smith, G. W., Lonergan, P., & Evans, A. C. O. (2007). Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle. Human Reproduction, 22(6), 1687-1695. doi: 10.1093/humrep/dem071

Lonergan, P., Rizos, D., Ward, F., & Boland, M. P. (2001). Factors influencing oocyte and embryo quality in cattle. Reproduction Nutrition Development, 41(5), 427-437. doi: 10.1051/rnd:2001142

Lowman, B. G. N., Scott, N. A., & Somerville, S. H. (1976). Condition scoring of cattle. Edinburgh: The Edinburgh School of Agriculture. (East of Scotland College of Agriculture. Bulletin.

Monteiro, F. M., Batista, E. O. S., Vieira, L. M., Bayeux, B. M., Accorsi, M., Campanholi, S. P.,… Baruselli, P. S. (2017). Beef donor cows with high number of retrieved COC produce more in vitro embryos compared with cows with low number of COC after repeated ovum pick-up sessions. Theriogenology, 90, 54-58. doi: 10.1016/j.theriogenology.2016.11.002

Mossa, F., Walsh, S. W., Butler, S. T., Berry, D. P., Carter, F., Lonergan, P.,… Evans, A. C. O. (2012). Low numbers of ovarian follicles ?3 mm in diameter are associated with low fertility indairy cows. Journal of Dairy Science, 95(5), 2355-2361. doi: 10.3168/jds.2011-4325

Parrish, J. J., Krogenaes, A., & Susko-Parrish, J. L. (1995). Effect of bovine sperm separation by either swim-upor Percoll method on success of in vitro fertilization and early embryonic development. Theriogenology, 44(6), 859-869. doi: 10.1016/0093-691x(95)00271-9

Pontes, J. H. F., Melo Sterza, F. A., Basso, A. C., Ferreira, C. R., Sanches, B. V., Rubin, K. C. P., & Seneda, M. M. (2011). Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology, 75(9), 1640-1646. doi: 10.1016/j.theriogenology.2010.12.026

Pontes, J. H. F., Nonato, I., Jr., Sanches, B. V., Ereno, J. C., Jr., Uvo, S., Barreiros, T. R. R.,... Seneda, M. M. (2009). Comparison of embryo yield and pregnancy rate between in vivo and in vitro methods in the same Nelore (Bos indicus) donor cows. Theriogenology, 71(4), 690-697. doi: 10.1016/j.theriogenology. 2008.09.031

Pontes, J. H. F., Silva, K. C. F., Basso, A. C., Rigo, A. G., Santos, G. M. G., Sanches, B. V.,... Seneda, M. M. (2010). Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus-taurus dairy cows using sexed sperm. Theriogenology, 74(8), 1349-1355. doi: 10.1016/j. theriogenology.2010.06.004

Rosa, C. O., Marinho, L., Rosa, P. da, Cesaro, M. P. de, Lunardelli, P. A., Silva-Santos, K. C.,... Seneda, M. M. (2018). Molecular characteristics of granulosa and cumulus cells and oocyte competence in Nelore cows with low and high numbers of antral follicles. Reproduction in Domestic Animals, 53(4), 921-929. doi: 10.1111/rda.13189

Santos, G. M. G., Silva-Santos, K. C., Barreiros, T. R. R., Blaschi, W., Morotti, F., Silva, C. B.,… Seneda, M. M. (2013). Conception rates following FTAI of Nelore cows (Bos indicus) with high, intermediate and low numbers of antral follicles. Animal Reproduction, 10(3), 451-451.

Santos, G. M. G., Silva-Santos, K. C., Barreiros, T. R. R., Morotti, F., Sanches, B. V., Moraes, F. L. Z.,… Seneda, M. M. (2016). High numbers of antral follicles are positively associated with in vitro embryo production but not the conception rate for FTAI in Nelore cattle. Animal Reproduction Science, 165, 17-21. doi: 10.1016/j.anireprosci.2015.11.024

Seneda, M. M., Esper, C. R., Garcia, J. M., Oliveira, J. A., & Vantini, R. (2001). Relationship between follicle size and ultrasound-guided transvaginal oocyte recovery. Animal Reproduction Science, 67(1-2), 37-43. doi: 10.1016/S0378-4320(01)00113-0

Silva-Santos, K. C., Santos, G. M., Koetz, C., Jr., Morotti, F., Siloto, L. S., Marcantonio, T. N.,... Seneda, M. M. (2014). Antral follicle populations and embryo production - in vitro and in vivo - of Bos indicus-taurus donors from weaning to yearling ages. Reproduction in Domestic Animals, 49(2), 228-232. doi: 10.1111/rda.12255

Sociedade Internacional de Transferência de Embriões (1998). Manual da sociedade internacional de transferência de embriões (3a ed.). Illinois.

DOI: http://dx.doi.org/10.5433/1679-0359.2020v41n5supl1p2171

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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