In vitro development of calla lily: effect of sucrose and gibberellic acid concentrations

Márcia de Nazaré Oliveira Ribeiro, Moacir Pasqual, Fabíola Villa

Abstract


Calla lily (Zantedeschia aethiopica) is appreciated as cut flower and for the composition of gardens. However, many pathogens affect this species. By the traditional method of propagation, some units of new seedlings can only be produced annually. Tissue culture allows fast large-scale clonal propagation and provides healthy uniform plants. During the in vitro process, type and concentration of growth regulator could affect the growth of seedlings. Thus, the aim of this work was to determine sucrose and GA3 concentrations to increase the efficiency of the in vitro multiplication of calla lily. After 60 days, the length of the above ground part and the roots, the number of sprouts, roots and leaves, above ground part and root fresh weight of seedlings were evaluated. The experimental design was entirely randomized with four replications. It was necessary the addition of 60.5 g L-1 sucrose associated to 5 mg L-1 GA3 to obtain hight sprouts number. For higher length of the above ground part the addition of 45.3 g L-1 sucrose and 10 mg L-1 GA3 was enough. Better results in the root length and number of roots were observed only in the sucrose presence, in concentrations in the range of 51.13 – 56.5 g L-1.

 

 

 


Keywords


Tissue culture; Carbohydrate; Growth regulator.



DOI: http://dx.doi.org/10.5433/1679-0359.2009v30n3p575

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional