Proliferative activity of oocytes in multi-oocyte follicles of bovine ovary

Reginaldo Luis Oliveira, Katia Cristina Silva-Santos, Suellen Miguez Gonzalez, Camila Bizarro-Silva, Fernanda Zandonadi Machado, Ana Paula Frederico Rodrigues Loureiro Bracarense, Marcelo Marcondes Seneda

Abstract


We characterized the proliferative activity of multi-oocyte follicles with anti-nuclear antigen of proliferating cells (PCNA). Ovaries (n = 12) from heifers were processed for histology. From 789 multi-oocytes follicles observed, only 11 were considered appropriated for immunostaining, since they presented all nuclei of the oocytes clearly visible. All multi-oocyte follicles were positive for PCNA, but some oocytes showed no proliferative activity. We conclude that oocytes in multi-oocyte follicles seem to be in different stages of the cell cycle.

Keywords


PCNA; Cattle; Folliculogenesis; Multiple oocytes.

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DOI: http://dx.doi.org/10.5433/1679-0359.2017v38n6p3591

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433 / 1679-0359
E-mail:  semina.agrarias@uel.br
Este obra está licenciado com uma Licença  Creative Commons Atribuição-NãoComercial 4.0 Internacional