Comparison of three Larval Immersion Tests in Syringe to evaluate acaricidal activity of chemical solutions

Juliana Antunes Farias, Claudia Cristina Gulias Gomes, Alessandro Pelegrine Minho, Robert Domingues, Bernardo Macke Franck, Rossana Leitzke Granada, Antonio Pereira de Souza

Abstract


The aim of this study was to compare Larval Immersion Test in syringes, originally proposed by Souza et al. (2008), with two modified bioassays proposed by Silva et al. (2011) and Sindhu et al. (2012). Rhipicephalus (Boophilus) microplus larvae were exposed to nine dilutions of commercial acaricide. After 24 hours, the number of dead larvae was counted. The LC50 for the three methods were 0.186%, 0.125% and 0.153%, respectively. Mortality of larvae exposed to chemical dilutions on Silva et al. (2011) were significantly higher than other methods at lower concentrations, probably in consequence of longer exposure time of larvae to solutions. However, modifications proposed by Sindhu et al. (2012) make technic execution more practical and safer.

Keywords


Syringe test; In vitro; Rhipicephalus (Boophilus) microplus.

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DOI: http://dx.doi.org/10.5433/1679-0359.2016v37n5p3205

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433 / 1679-0359
E-mail:  semina.agrarias@uel.br
Este obra está licenciado com uma Licença  Creative Commons Atribuição-NãoComercial 4.0 Internacional