Effect of catalase, superoxide dismutase and reduced glutathione in LDL extender on ovine cryopreserved sperm viability

Autores

  • Paola Pereira das Neves Snoeck Universidade Estadual de Santa Cruz
  • Luís Cláudio Oliveira Moura Universidade Estadual de Santa Cruz
  • Carlos Augusto Alanis Clemente Universidade Federal de Minas Gerais
  • Ana Maria Loaiza-Echeverri Universidade Federal de Minas Gerais
  • Mariana Machado Neves Universidade Federal de Viçosa
  • Ivan Bezerra Allaman Universidade Estadual de Santa Cruz
  • Marc Henry Universidade Federal de Minas Gerais

DOI:

https://doi.org/10.5433/1679-0359.2015v36n4p2593

Palavras-chave:

Cryopreservation, Semen, Enzymatic antioxidants, Santa Inês.

Resumo

 

The aim of this study was to evaluate the motility, kinetics and membrane integrity of ovine sperm cryopreserved in extenders containing 8% LDL with enzymatic antioxidants at different concentrations. Four Santa Inês rams were used to form four pools of semen (each pool containing ejaculates from four ram, totaling four ejaculates per animal). Each seminal pool was divided into eight aliquots for the following treatments: 1) Tris-glucose-glycerol (TGG) + (16%) egg yolk (control 1); 2) TGG + 8% (w/v) LDL (control 2); 3) TGG + 8% LDL + catalase 100 U/mL; 4) TGG + 8% LDL + catalase 200 U/mL; 5) TGG + 8% LDL + superoxide dismutase 100 U/mL; 6) TGG + 8% LDL + superoxide dismutase 200 U/mL; 7) TGG + 8% LDL + reduced glutathione 5 mM; and 8) TGG + 8% LDL + reduced glutathione 10 mM. The samples were packed into 0.25 mL straws, cooled (-0.25 °C/ min), maintained at 5 °C for 2 h and then frozen (-25 °C/ min) using a TK4000®. Immediately after thawing (38 °C/ 30 s), sperm motility and movement characteristics were assessed by computer sperm analysis (CASA). The structural integrity of the plasma and acrosomal membranes was analyzed using fluorescent dyes. The functional integrity of membranes was assessed using a hypoosmotic swelling test. As assessed by ANOVA, significant differences (P<0.05) among treatments were only observed for VCL, VSL and VAP. For the VCL variable, the 2, 3, 4, 5, 6 and 7 extenders were similar and higher than 1 and 8 extenders, the latter being similar to each other. For the VSL variable, the 3, 4, 5, 6 and 7 extenders were similar and higher than 1, 2 and 8 extenders, the latter being similar to each other. For the VAP variable, the 3, 4 and 6 extenders were similar and higher than 1, 2, 5, 7 and 8 extenders, the latter being similar to each other. In conclusion, enzymatic antioxidants as catalase e superoxide dismutase improve the protective activity of extenders containing LDL on frozen ovine sperm.

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Biografia do Autor

Paola Pereira das Neves Snoeck, Universidade Estadual de Santa Cruz

Prof., Universidade Estadual de Santa Cruz, UESC, Ilhéus, BA. Brasil.

Luís Cláudio Oliveira Moura, Universidade Estadual de Santa Cruz

Discente, UESC, Ilhéus, BA, Brasil.

Carlos Augusto Alanis Clemente, Universidade Federal de Minas Gerais

Prof., Universidade Federal da Paraíba, UFPB, Bananeiras, PB, Brasil.

Ana Maria Loaiza-Echeverri, Universidade Federal de Minas Gerais

Pesquisadora, Universidade Federal de Minas Gerais, UFMG, Belo Horizonte, MG, Brasil.

Mariana Machado Neves, Universidade Federal de Viçosa

Profª, UFV, Viçosa, MG, Brasil.

Ivan Bezerra Allaman, Universidade Estadual de Santa Cruz

Pesquisador, UESC, Ilhéus, BA, Brasil.

Marc Henry, Universidade Federal de Minas Gerais

Prof., UFMG, Belo Horizonte, MG, Brasil.

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Publicado

2015-08-13

Como Citar

Snoeck, P. P. das N., Moura, L. C. O., Clemente, C. A. A., Loaiza-Echeverri, A. M., Neves, M. M., Allaman, I. B., & Henry, M. (2015). Effect of catalase, superoxide dismutase and reduced glutathione in LDL extender on ovine cryopreserved sperm viability. Semina: Ciências Agrárias, 36(4), 2593–2602. https://doi.org/10.5433/1679-0359.2015v36n4p2593

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