Helicobacter spp. infection in dogs is not associated with changes in epithelial proliferation or E-cadherin expression in gastric mucosa

Autores

  • Luciana Sayuri Takemura Universidade Estadual de Londrina
  • Romulo Lot Faculdade de Medicina Veterinária e Zootecnia de Garça
  • Pedro Luiz Camargo Universidade Estadual de Londrina
  • Elisangela Silva Universidade Estadual de Londrina
  • Amauri Alcindo Alfieri Universidade Estadual de Londrina
  • Ana Paula Frederico Rodrigues Loureiro Bracarense Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2012v33n6Supl2p3211

Palavras-chave:

Dog, Gastric lesion, Helicobacter spp., Epithelial proliferation, Cell adhesion.

Resumo

Helicobacter pylori infection causes gastritis and can induce gastric adenocarcinoma and MALT lymphoma in humans. The aim of this study was to determine in dogs whether there is an association between Helicobacter spp. infection in gastric mucosa, histological lesions, including epithelial cell proliferation and cell adhesion. Gastric biopsies of 12 dogs with gastric disturbances and 25 healthy dogs were evaluated. Warthin-Starry staining (WS) and PCR assay were performed to confirm the presence of helicobacteria. The Helicobacter species were determined by PCR assay with speciesspecific primers for H. heilmannii, H. bizzozeronii or H. salomonis, H. felis and H. pylori. Mucosal lesions were evaluated by haematoxylin and eosin (HE) and epithelial proliferation was determined by AgNOR and PCNA methods. Cell adhesion was evaluated by the expression of E-cadherin by epithelial cells. Helicobacter spp. was confirmed in 75.7% (28/37) and 73.0% (27/37) of the samples by WS and PCR, respectively. H. bizzozeronii was the species most frequently detected (37%); co-infection was observed in six (22%) dogs. Histological changes in the lamina propria included mild chronic gastritis, fibrosis, glandular degeneration, and presence of lymphoid aggregates. There was a significant association between H. heilmannii infection and the presence of lymphoid follicles (p < 0.046). In this study no significant change in epithelial proliferation or E-cadherin expression were detected in the gastric mucosa of dogs infected with Helicobacter spp.

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Biografia do Autor

Luciana Sayuri Takemura, Universidade Estadual de Londrina

Prof. Adjunto, Universidade Norte do Paraná, UNOPAR, Londrina, PR.

Romulo Lot, Faculdade de Medicina Veterinária e Zootecnia de Garça

Prof. Assistente, Faculdade de Medicina Veterinária e Zootecnia de Garça, Garça, SP.

Pedro Luiz Camargo, Universidade Estadual de Londrina

Prof. Dr. Associado, Deptº de Clínicas Veterinárias. UEL, Universidade Estadual de Londrina, UEL, Londrina, PR.

Elisangela Silva, Universidade Estadual de Londrina

Discente de Mestrado em Ciência Animal, UEL, Londrina, PR.

Amauri Alcindo Alfieri, Universidade Estadual de Londrina

Prof. Dr. Associado, Deptº Medicina Veterinária Preventiva, UEL, Londrina, PR.

Ana Paula Frederico Rodrigues Loureiro Bracarense, Universidade Estadual de Londrina

Profª. Drª Associado, Deptº Medicina Veterinária Preventiva, UEL, Londrina, PR.

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Publicado

2013-02-28

Como Citar

Takemura, L. S., Lot, R., Camargo, P. L., Silva, E., Alfieri, A. A., & Bracarense, A. P. F. R. L. (2013). Helicobacter spp. infection in dogs is not associated with changes in epithelial proliferation or E-cadherin expression in gastric mucosa. Semina: Ciências Agrárias, 33(6Supl2), 3211–3222. https://doi.org/10.5433/1679-0359.2012v33n6Supl2p3211

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