A sneak peek into chromosome and genome size evolution among sisyrynchium taxa (Iridaceae: Iridoieae) SISYRINCHIUM TAXA (IRIDACEAE: IRIDOIDEAE)

Autores

  • Paula Burchardt Universidade Federal do Rio Grande do Sul
  • Tatiana Teixeira Souza Chies Universidade Federal do Rio Grande do Sul
  • Lauís Brisolara Corrêa Universidade Federal do Rio Grande do Sul
  • Olivier Chauveau Universidade Federal do Rio Grande do Sul
  • Camila Dellanhese Inácio Universidade Federal do Rio Grande do Sul
  • Lilian Eggers
  • Sonja Siljak Yakovlev Université Paris-Saclay
  • José Marcello Salabert de Campos Universidade Federal de Juiz de Fora
  • Eliane Kaltchuk Santos Universidade Federal do Rio Grande do Sul

DOI:

https://doi.org/10.5433/1679-0367.2017v38n1suplp65

Palavras-chave:

Ancestral chromosome number, DNA content, Meiotic behavior, Pollen viability, Polyploidy

Resumo

Iridaceae is one of the largest Asparagales families with over 2030 species. Besides a wide morphological variation, this family presents great karyological variation including many polyploid taxa. Sisyrinchium is restricted to the New World and it holds a large amount of cytological data. Despite its importance in providing critical evidence to understand processes involved in genome evolution, cytological data is lacking for most of South American species of Sisyrinchium. Therefore, the present work aims to identify and characterize chromosome evolutionary trends among Sisyrinchium taxa of sect. Viperella. Chromosome numbers and genome sizes (GS) were determined for 16 and 17 taxa, respectively, from sect. Viperella; all reported here for the first time, except for S. alatum. Meiotic behavior, tetrad normality, pollen viability and morphology were also analyzed and revealed regular meiosis, high meiotic indexes and pollen viability (> 87%), except for Sisyrinchium sp. nov. aff. alatum (22.70%). The GS and chromosome number obtained were plotted onto a phylogenetic frame to identify transitions between character states and to locate where these transitions occurred throughout this group’s evolution. Based on our results, we suggest that the ancestral chromosome number for sect. Viperella is 2n = 18 and two independent polyploidization events occurred. Lower DNA content (2.17 ? 2C ? 2.97 pg) was the ancestral condition from which six major events took place towards GS increase. Despite the stability in base chromosome number (x = 9), evolutionary changes in karyotypes of this section involve primarily variation in DNA amount regardless of ploidy level, and secondly polyploidy and variation in chromosome size. Combining GS and chromosome number with molecular phylogeny, our data provides the first scenario for karyotype and genome evolution in Sisyrinchium, sect. Viperella, allowing us to suggest that mechanisms other than polyploidy were responsible for GS increase among these taxa.

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Biografia do Autor

Paula Burchardt, Universidade Federal do Rio Grande do Sul

Programa de Pós-Graduação em Genética e Biologia Molecular, UFRGS, Porto Alegre, RS, Brazil

Tatiana Teixeira Souza Chies, Universidade Federal do Rio Grande do Sul

Programa de Pós-Graduação em Genética e Biologia Molecular e Departamento de Botânica, Instituto de Biociências, UFRGS, Porto Alegre, RS, Brazil

Lauís Brisolara Corrêa, Universidade Federal do Rio Grande do Sul

Programa de Pós-Graduação em Genética e Biologia Molecular, UFRGS, Porto Alegre, RS, Brazil

Olivier Chauveau, Universidade Federal do Rio Grande do Sul

Programa de Pós-Graduação em Botânica, Instituto de Biociências, UFRGS, Brazil

Camila Dellanhese Inácio, Universidade Federal do Rio Grande do Sul

Programa de Pós-Graduação em Botânica, Instituto de Biociências, UFRGS, Brazil

Lilian Eggers

Departamento de Botânica, Instituto de Biociências e Programa de Pós-Graduação em Botânica, Instituto de Biociências, UFRGS, Brazil

Sonja Siljak Yakovlev, Université Paris-Saclay

Ecologie Systématique Evolution, Univ. Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, Orsay Cedex, France.

José Marcello Salabert de Campos, Universidade Federal de Juiz de Fora

Departamento de Biologia, Instituto de Ciências Biológicas, UFJF, Juiz de Fora, MG, Brazil

Eliane Kaltchuk Santos, Universidade Federal do Rio Grande do Sul

Programa de Pós-Graduação em Genética e Biologia Molecular e Departamento de Genética, Instituto de Biociências, UFRGS, Brazil.

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Publicado

2018-02-16

Como Citar

1.
Burchardt P, Souza Chies TT, Brisolara Corrêa L, Chauveau O, Inácio CD, Eggers L, Siljak Yakovlev S, Campos JMS de, Kaltchuk Santos E. A sneak peek into chromosome and genome size evolution among sisyrynchium taxa (Iridaceae: Iridoieae) SISYRINCHIUM TAXA (IRIDACEAE: IRIDOIDEAE). Semin. Cienc. Biol. Saude [Internet]. 16º de fevereiro de 2018 [citado 28º de março de 2024];38(1supl):65. Disponível em: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/29481