Characterization of satDNAs in Mahanarva quadripunctata (Cercopidae) through high-throughput and chromosomal analysis

Autores

  • Allison Anjos Universidade Estadual de São Paulo
  • Diogo Milani Universidade Estadual de São Paulo
  • Andressa Palladini Universidade Federal de Santa Maria
  • Diogo Cavalcanti Cabral de Mello Universidade Estadual de São Paulo

DOI:

https://doi.org/10.5433/1679-0367.2017v38n1suplp123

Palavras-chave:

FISH, Next-generation Sequencing, Spittlebugs, Satellite DNA

Resumo

SSpittlebugs (Auchenorrhyncha) are a group of insects best known to causes losses in agricultural and pasture grasses by feeding plant fluids. Despite the great karyotypic variability described for organisms with holocentric chromosomes, as observed in Auchenorrhyncha, genomic organization of repetitive DNA sequences, such as Satellite DNAs remains poorly studied. Due to its abundance in the eukaryotic genomes, the obtaining of markers based in satDNAs could improve the understanding of karyotype evolution. Here using Illumina next-generation sequencing and graph-based clustering we performed the characterization of satDNA families prospected from the genome of Mahanarva quadripunctata (2n=19, X0?). Moreover, we tested the occurrence of the two most abundant satDNA in M. quadripunctata in other species of Auchenorrhyncha. A total of 30 satDNA families were found through Illumina NGS and RepeatExplorer analyses, representing 4.53% of the M. quadripunctata genome. The eight successfully isolated satDNAs were labeled and used as probes for fluorescent in situ hybridization, resulting in several distribution patterns as follows: (I) two satDNAs spread to the whole chromosome complement, forming band-like pattern; (II) two satDNAs with clusters in one autosomal bivalent; (III) three satDNAs interspersed across the whole genome without observation of conspicuous bands; (IV) one satDNA with no signals detected by FISH. Dot blot using genomic DNA from six Mahanarva and other eight Auchenorrhyncha species revealed positive hybridization only against the genomic DNA of Mahanarva species for the two most abundant satDNAs, indicating common origin, at least, in Mahanarva genus in accordance to “Library hypothesis”. The data suggest complex organization for distinct families of satDNAs, at chromosomal level, suggesting divergent patterns of clusterization and evolution

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Biografia do Autor

Allison Anjos, Universidade Estadual de São Paulo

Grupo de Estudos em Citogenômica e Evolução Animal - Universidade Estadual de São Paulo, Rio Claro/SP

Diogo Milani, Universidade Estadual de São Paulo

Grupo de Estudos em Citogenômica e Evolução Animal - Universidade Estadual de São Paulo, Rio Claro/SP

Andressa Palladini, Universidade Federal de Santa Maria

Departamento de Ecologia e Evolução, Universidade Federal de Santa Maria, Santa Maria, RS

Diogo Cavalcanti Cabral de Mello, Universidade Estadual de São Paulo

Grupo de Estudos em Citogenômica e Evolução Animal - Universidade Estadual de São Paulo, Rio Claro/SP

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Publicado

2018-02-16

Como Citar

1.
Anjos A, Milani D, Palladini A, Cabral de Mello DC. Characterization of satDNAs in Mahanarva quadripunctata (Cercopidae) through high-throughput and chromosomal analysis. Semin. Cienc. Biol. Saude [Internet]. 16º de fevereiro de 2018 [citado 29º de março de 2024];38(1supl):123. Disponível em: https://ojs.uel.br/revistas/uel/index.php/seminabio/article/view/29308