Characterization of satDNAs in Mahanarva quadripunctata (Cercopidae) through high-throughput and chromosomal analysis

Allison Anjos, Diogo Milani, Andressa Palladini, Diogo Cavalcanti Cabral de Mello

Resumo


SSpittlebugs (Auchenorrhyncha) are a group of insects best known to causes losses in agricultural and pasture grasses by feeding plant fluids. Despite the great karyotypic variability described for organisms with holocentric chromosomes, as observed in Auchenorrhyncha, genomic organization of repetitive DNA sequences, such as Satellite DNAs remains poorly studied. Due to its abundance in the eukaryotic genomes, the obtaining of markers based in satDNAs could improve the understanding of karyotype evolution. Here using Illumina next-generation sequencing and graph-based clustering we performed the characterization of satDNA families prospected from the genome of Mahanarva quadripunctata (2n=19, X0?). Moreover, we tested the occurrence of the two most abundant satDNA in M. quadripunctata in other species of Auchenorrhyncha. A total of 30 satDNA families were found through Illumina NGS and RepeatExplorer analyses, representing 4.53% of the M. quadripunctata genome. The eight successfully isolated satDNAs were labeled and used as probes for fluorescent in situ hybridization, resulting in several distribution patterns as follows: (I) two satDNAs spread to the whole chromosome complement, forming band-like pattern; (II) two satDNAs with clusters in one autosomal bivalent; (III) three satDNAs interspersed across the whole genome without observation of conspicuous bands; (IV) one satDNA with no signals detected by FISH. Dot blot using genomic DNA from six Mahanarva and other eight Auchenorrhyncha species revealed positive hybridization only against the genomic DNA of Mahanarva species for the two most abundant satDNAs, indicating common origin, at least, in Mahanarva genus in accordance to “Library hypothesis”. The data suggest complex organization for distinct families of satDNAs, at chromosomal level, suggesting divergent patterns of clusterization and evolution

Palavras-chave


FISH; Next-generation Sequencing; Spittlebugs; Satellite DNA

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DOI: http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp123

Direitos autorais 2018 Semina: Ciências Biológicas e da Saúde

Licença Creative Commons
Esta obra está licenciada sob uma licença Creative Commons Atribuição - NãoComercial 4.0 Internacional.

Semina: Ciênc. Biol. Saúde

email: seminabio@uel.br

Londrina - PR
ISSN Print: 1676-5435

EISSN: 1679-0367