Development of a method for determination of fatty acid using FT-IR spectroscopy

Development of a method for determination of fatty acid using FT-IR spectroscopy

Authors

  • Dimas Augusto Morozin Zaia Universidade Estadual de Londrina
  • Leonardo de Oliveira Guarnieri Universidade Estadual de Londrina
  • Karina Nicole Sobota Universidade Estadual de Londrina
  • Henrique de Santana Universidade Estadual de Londrina
  • Cássia Thaïs Bussamra Vieira Zaia Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0375.2011v32n1p79

Keywords:

Fatty acids, Palmitic acid, Biological systems, FT-IR Spectroscopy

Abstract

In the present paper a new methodology has been developed for determination of fatty acids in biological systems using FT-IR spectroscopy. For this method is not necessary chromophore reagent or pre sample preparation. Palmitic acid was chosen as standard, because it is found in several biological systems. The FT-IR spectrum of palmitic acid showed two absorption bands in the region of 2852 and 2920 cm-1 attributed to CH stretching. The results for these bands showed that the Beer-Lambert Law was followed in wide range of concentration of palmitic acid (14 to 257 mmol L-1). Potassium ferricyanide (K3[Fe(CN)6]) was used as internal standard. Several interferents were tested and only cholesterol, ferric chloride (higher concentration), mixture of amino acids for the band at 2919 cm-1 (higher concentration) and triglyceride could be interferent if they appear in high concentration. Thus, this new methodology has advantage to be not expensive and simple.

Metrics

Metrics Loading ...

Author Biographies

Dimas Augusto Morozin Zaia, Universidade Estadual de Londrina

Professor Associado-C, Departamento de Química-CCE

Leonardo de Oliveira Guarnieri, Universidade Estadual de Londrina

Bacharelando em Biomedicina, Departamento de Ciências Fisiológicas-CCB

Karina Nicole Sobota, Universidade Estadual de Londrina

Bacharelando em Biomedicina, Departamento de Ciências Fisiológicas-CCB

Henrique de Santana, Universidade Estadual de Londrina

Professor Associado-C, Departamento de Química-CCE

Cássia Thaïs Bussamra Vieira Zaia, Universidade Estadual de Londrina

 

 Professora Associada-C, LaFiNeM-Laboratório de Fisiologia Neuroendócrina e metabolismo, Departamento de Ciências Fisiológicas-CCB, Universidade Estadual de Londrina

Published

2011-07-15

How to Cite

Zaia, D. A. M., Guarnieri, L. de O., Sobota, K. N., de Santana, H., & Zaia, C. T. B. V. (2011). Development of a method for determination of fatty acid using FT-IR spectroscopy. Semina: Ciências Exatas E Tecnológicas, 32(1), 79–86. https://doi.org/10.5433/1679-0375.2011v32n1p79

Issue

Section

Original Article

Most read articles by the same author(s)

Loading...