Pulp fruit added to culture medium for in vitro orchid development

Autores

  • Gilberto Rostirolla Batista de Souza Universidade Estadual Paulista Júlio de Mesquita Filho
  • Alessandro Borini Lone Universidade Estadual de Londrina
  • Ricardo Tadeu de Faria Universidade Estadual de Londrina
  • Karen Sinéia de Oliveira Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2013v34n3p1141

Palavras-chave:

Alternative culture medium, Cattleya bicolor Lindl., Conservation, Subculture, Coconut.

Resumo

As an additive in in vitro culture media, fruits have a great potential for facilitating economical orchid production because of lower technology requirements and the ease of obtaining raw materials to formulate culture media. We studied the in vitro growth of Cattleya bicolor Lindl. grown in a simplified culture medium supplemented with different kinds of fruit pulp. The experimental design was completely randomised, with eight seedlings per replication and ten replications per treatment, for a total of 80 seedlings per treatment. The culture medium was made using 150 g L -1 of pulp (without peel or seed) from the following fruits: ripe Santa Cruz tomatoes (Solanum lycopersicum L.), dwarf bananas (Musa cavendishii L.) of intermediate ripeness, light green chayote (Sechium edule (Jacq.) Sw), ripe papaya (Carica papaya L.) or green coconut (Cocos nucifera L.).The treatment control was MS 50 %. The treatments and the control were kept in a growth chamber for seven months before evaluating seedling survival percentage, shoot height, number of leaves, rooting percentage, root number, root length and dry masses of shoot and roots. The highest percentages of seedling survival were obtained using MS 50 %, banana and coconut medium. The seedling survival and rooting percentages illustrate that it is possible to emphasise the culture medium MS 50% and the culture medium supplemented with coconut on the most traditional culture medium with banana or tomato pulp. For the in vitro development of Cattleya bicolor Lindl., a simplified culture medium supplemented with coconut pulp is the most suitable for use as an alternative to MS 50%. A simplified culture medium supplemented with papaya pulp is not recommended for the in vitro development of Cattleya bicolor Lindl.

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Biografia do Autor

Gilberto Rostirolla Batista de Souza, Universidade Estadual Paulista Júlio de Mesquita Filho

Centro de Ciências Agrárias, Departamento de Agronomia, Fitotecnia - Floricultura.

Alessandro Borini Lone, Universidade Estadual de Londrina

Discente de Doutorado em Agronomia, Universidade Estadual de Londrina, UEL, Londrina, PR.

Ricardo Tadeu de Faria, Universidade Estadual de Londrina

Eng° Agrº, Prof. Dr. Associado do Deptº de Agronomia,UEL, Londrina, PR.

Karen Sinéia de Oliveira, Universidade Estadual de Londrina

Discente de graduação em Agronomia, UEL, Londrina, PR.

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Publicado

2013-06-24

Como Citar

Souza, G. R. B. de, Lone, A. B., Faria, R. T. de, & Oliveira, K. S. de. (2013). Pulp fruit added to culture medium for in vitro orchid development. Semina: Ciências Agrárias, 34(3), 1141–1146. https://doi.org/10.5433/1679-0359.2013v34n3p1141

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