Oxidative stress profile in goats supplemented or not with selenium and vitamin E and submitted to scrotal insulation

Janaína Azevedo Guimarães, Guadalupe Carvalho Xavier, Pierre Castro Soares, Alexandre Cruz Dantas, Filipe Aureliano Pedrosa Soares, Carla Lopes Mendonça, Maria Madalena Pessoa Guerra, Valdemiro Amaro Silva Júnior

Abstract


To evaluate the effect of supplementation with selenium and vitamin E in the diet of goats induced to scrotal insulation (SI) on the profile of biochemical markers of systemic oxidative metabolism 12 animals were randomly divided into two groups: G1 - no supplementation G2 - supplemented with selenium (0.1 mg / kg body weight of sodium selenite) and vitamin E (0.3 IU / kg body weight). At the end of the adjustment period of 30 days, the scrotal insulation (SI) was performed with the placement of plastic bags in the testes for 18 days. Supplementation with Se + vitamin E began 60 days before induction of SI and maintained for 42 days after the end of SI, corresponding to post-insulation (PSI). Blood samples were obtained by jugular venipuncture to obtain total blood for analysis of reduced glutathione (GSH) and plasma for analysis of thiobarbituric acid reactive substance (TBARS) and determination of ferric reducing ability of plasma (FRAP). No effect of dietary supplementation with Se + vitamin E on the profile of all the variables studied, but there was systemic oxidative action understood by a significant decrease of GSH during the SI, with a gradual increase in the PSI period, as well as lower average of FRAP observed during the SI and the highest average in the period from PSI. It was concluded that analysis of the activity of reduced glutathione (GSH) and concentrations of thiobarbituric acid reactive substances (TBARS) and ferric reducing ability of plasma (FRAP) are recommended laboratory methods to evaluate the systemic response to oxidative damage caused by thermal stress testis in goats.


Keywords


Testis; Goat; Heat stress; Enzymes; Antioxidants.



DOI: http://dx.doi.org/10.5433/1679-0359.2011v32n4Sup1p1955

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional