Ultrastructural analysis of bovine oocytes from ovarian follicles with different diameters

Autores

  • Lívia Aires Lisboa Universidade Estadual de Londrina
  • Evelyn Rabelo Andrade Universidade Estadual de Londrina
  • Cesar Roberto Esper Universidade Estadual Paulista
  • Marcelo Marcondes Seneda Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2011v32n4p1575

Palavras-chave:

Follicle size, OPU, Electron microscopy.

Resumo

In vitro embryo production is an important technique for facilitating the reproduction of animals with high genetic merit. The greatest challenge for the reproducibility of this technique is the quality of the oocyte that is submitted for in vitro maturation. The aim of this work was to evaluate the ultrastructural characteristics of oocytes from follicles of different diameters using transmission electron microscopy. The animals were divided into 2 groups and were given a single i.m. injection of 250 IU FSH (Pluset, Serono, Italy). To synchronize the follicular growth, all follicles > 2 mm were aspirated during the estrous cycle, which was considered day zero (D0). Group 1 (G1; n = 4) received FSH on day 1 (D1), and the 2- to 5-mm follicles were aspirated on day 2 (D2). The animals in group 2 (G2; n = 5) received FSH on day 2 (D2), and their 10- to 15-mm follicles were aspirated on day 5 (D5). After aspiration, the oocytes from both groups were fixed and prepared for ultrastructural analysis. The oocytes analyzed from both groups had similar ultrastructural characteristics. The presence and distribution of organelles in the cytoplasm of the oocytes did not differ between groups, suggesting that, in relation to the ultrastructural characteristics, oocytes from 2 to 5 mm and 10 to 15 mm follicles are similar.

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Biografia do Autor

Lívia Aires Lisboa, Universidade Estadual de Londrina

Doutoranda na área de Reprodução Animal, Universidade Estadual de Londrina, UEL. Londrina, PR.

Evelyn Rabelo Andrade, Universidade Estadual de Londrina

Pós-doutoranda na área de Reprodução Animal, UEL. Londrina, PR.

Cesar Roberto Esper, Universidade Estadual Paulista

Prof. Dr. do Deptº de Medicina Veterinária Preventiva e Reprodução Animal da Universidade Estadual Paulista, UNESP, São Paulo, SP.

Marcelo Marcondes Seneda, Universidade Estadual de Londrina

Prof. Dr. do Deptº de Clínicas Veterinárias, Centro de Ciências Agrárias, UEL, Londrina, PR.

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Publicado

2011-10-14

Como Citar

Lisboa, L. A., Andrade, E. R., Esper, C. R., & Seneda, M. M. (2011). Ultrastructural analysis of bovine oocytes from ovarian follicles with different diameters. Semina: Ciências Agrárias, 32(4), 1575–1582. https://doi.org/10.5433/1679-0359.2011v32n4p1575

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