Erythrogram, oxidative stress and mineral interaction in naturally infected lambs supplemented with different forms of oral iron

Ricardo Xavier Rocha, Emmanuel Veiga Camargo, Diego Zeni, Paula Rocha Sampaio Nicolodi, Marta Lizandra Rêgo Leal, Marcelo Silva Cecim

Abstract


The present study aimed to assess the oxidative profile, erythrogram and mineral interaction in lambs with anemia due to worm infection supplemented with different forms of oral iron. It was used 27 lambs, 6 to 8 month old, naturally infected by Haemonchus contortus, which showed packed cell volume between 16 and 18%. The animals were divided in three groups: Control Group (GC) n=9, Ferrous Sulphate Group (G2) n=9 and Ferric Sulphate Group (G3) n=9. The animals of G2 received 1 g of ferrous sulphate (Fe+2) orally daily, equivalent to 200 milligram of iron, the animals of G3 received 1 g of ferric sulphate (Fe+3) orally daily, or equivalent to 200 milligram of iron, whereas the GC received no treatment. The samples were taked on day 0, 7, 14, 21 and 28 of the experiment, and during four days two animals of each group were kept in metabolic cages to measure the faecal minerals excretion. There was no difference among the groups about serum iron values and parameters of red blood cells. The serum copper and zinc values were lower in the G2 and G3 on days 21 and 28 of the experiment, whereas the faecal copper, iron and zinc excretion was higher in the same groups. The superoxide dismutase (SOD) levels were lower in the G2 and G3 on day 28 whereas the levels of non-protein thiol groups (NPTH) showed a decrease on days 21 and 28. In relation to reactive species thio-barbituric acid (TBARS), there was an increase on day 28 in the G2 and G3. Based on these results, it was concluded that the oral supplementation with 200 mg of iron, irrespective of its form, ferrous or ferric, does not increase the erythrocyte response in lambs. As well as, it has antagonist action on copper and zinc, reducing its serum concentrations and increasing the faecal excretion of these minerals. Moreover, the decrease of the serum copper and zinc concentrations causes a decrease in activity of superoxide dismutase, causing an oxidative stress situation.


Keywords


Sheep; Supplements; Minerals; Oxidative stress.



DOI: http://dx.doi.org/10.5433/1679-0359.2012v33n2p723

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional