Seroprevalence of Cutaneous Leishmaniasis (LC) in dogs and identification of vectors (Diptera:Psychodidae) in Bela Vista do Paraíso, Parana state

Homero Ribeiro dos Reis, Fabiana Maria Ruiz Lopes-Mori, Célia Rosimarie dos Reis, Roberta Lemos Freire, Elizabete Regina Marangoni Marana, Andreas Lazaro Chryssafidis, André Vieira Tedim, Bruno Bergamo Ruffolo, Felipe Monteiro Bugni, Edilene Alcântara de Castro, Vanete Thomaz-Soccol, Luciene Biazzono Nabut, Italmar Teodorico Navarro

Abstract


The aim of this study was to determine the seroprevalence of Cutaneous Leishmaniasis (CL) in dogs of Bela Vista do Paraiso, Parana state, compare the IFA and ELISA techniques and identify the vectors possibly involved in the cycle of the parasite. Were collected blood samples from 489 dogs that were subjected to detection of anti-Leishmania sp. by IFA and by ELISA. Were considered positive samples ³40 titers in IFA and for ELISA ³ 0.174 optical density. Among the samples analyzed, 222 (45.4%) were positive by IFA and 189 (38.7%) by ELISA. Comparing the tests were found 176 positive samples (36.0%) and 254 negative (51.9%) for both techniques. The sensitivity of ELISA was 79.3% and specificity was 95.1%. The global coefficient of the test was 87.0% with kappa coefficient of 0.75. Analysis of variables for dogs with positive serology by IFA showed significant differences regarding the absence of forests and lack of contact with other animal species. Positive sera by ELISA in dogs variables that showed significant differences were the type of riparian vegetation existing in the environment, the lack of garbage, open sewers and released directly into rivers or streams and garbage thrown on wasteland, burned or buried. The result obtained with the capture of sandflies was the predominance of Lutzomyia whitmani with 79.9% of the species collected. The result obtained with the capture of sandflies was the predominance of Lutzomyia whitmani with 79.9% of the species collected. The results showed that the LTA is widespread in the canine population of Bela Vista do Paraiso, and both the IFI and ELISA can be used for diagnosis. So the dog appears as a link between wild and peridomestic cycle of CL may become an amplifier of disease in this ecosystem.


Keywords


Leishmania sp.; Dogs; Sorology; IFA; ELISA; Lutzomyia.



DOI: http://dx.doi.org/10.5433/1679-0359.2011v32n3p1083

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional