Staphylococcal enterotoxin for standard production and remotion of food interferents in RPLA assay

Claudia Moreno Rosa, Tereza Cristina Rocha Moreira Oliveira, Elisa Yoko Hirooka

Abstract


The frequency of staphylococcal intoxication requires constant improvements to provide more sensitive and practical assays for enterotoxins detection in foods. Considering that such an advantage is always related to an unexpected events, this view point approaches about recent available resources to reduce the problems associated with new techniques. Emphasis was appointed to the culture formulations for enterotoxin purification, as the remotion of food components which interfire with the most simplied method of enterotoxin detection, the latex agglutination assay- RPLA.

 


Keywords


Staphylococci; Enterotoxin; Culture medium; Affinity chromatography; Extraction.



DOI: http://dx.doi.org/10.5433/1679-0359.1999v20n1p71

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional