Development of a qPCR to diagnose the genus Eimeria in bovines

Sérgio Tosi Cardim, Stefani Lino Cardim, Mércia de Seixas, Alessandra Taroda, Priscilla Gomes Carneiro, Dauton Luiz Zulpo, Luiz Daniel de Barros, Guilherme Luis Bortolloti, João Luis Garcia

Abstract


Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry).


Keywords


qPCR; Eimeria; coccidiosis; 18S rRNA; cattle

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References


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DOI: http://dx.doi.org/10.5433/1679-0359.2020v41n6p2695

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional