Effect of additional PGF2? dose on pregnancy rate in Nellore females subjected to FTAI

Jorge Luís Ferreira, Matheus Henrique Dias Rodrigues, Shammara Noleto Santos, Rodolfo Olinto Rotoli Garcia de Oliveira, Vanessa de Sousa Rodrigues, Dourival Alex Araújo Beserra, José Américo Soares Garcia

Abstract


This study examined the effect of administering an additional dose of prostaglandin F2 alpha (PGF2?), in a fixed-time artificial insemination protocol (FTAI), on the fertility of female Nellore cattle. Two experiments were carried out: the first (Experiment I) took place in the state of Tocantins and the second (Experiment II) in the state of Pará, Brazil. In Experiment I (E1), 80 cows were used in three treatments (T1, T2 and T3) in which all received the same FTAI protocol. In T1 (n = 29), the cows received 12.5 mg of Dinoprost on day 9; in T2 (n = 28), they received the additional dose on day 10; and in T3 (n = 23; control group), the animals did not receive the additional PGF2? dose. Experiment II consisted of 147 bovine females distributed into two treatment groups, namely, T1 - 72 animals receiving the same protocol as T1 of E1; and T2 - 75 animals receiving the same protocol as T3 of E1. Statistical analysis was performed using SAS software, applying the PROC NPARWAY procedure for E1, and means were compared by the Wilcoxon test at the 5% significance level. In Experiment II, the data were subjected to analysis of variance by PROC GLIMMIX and means were compared by the T test at the 5% significance level. The following pregnancy rates were obtained in Experiment I: T1 - 62.06% (18/29); T2 - 57.14% (16/28); and T3 - 52.17% (12/23), with no significant difference observed between treatments. In Experiment II, pregnancy rate in T1 was 66.67% (48/72), whereas in T2 it was 41.33% (31/75), with a significant difference detected (P < 0.05). An additional dose of PGF2? provides an increase in pregnancy rate in Nellore females.

Keywords


Bovine; FTAI; PGF2alfa; Pregnancy rate.

Full Text:

PDF

References


Baruselli, P. S., SÁ Filho, M. F., Ferreira, R. M., Sales, J. N., Gimenes, L. U., Vieira, L. M., Mendanha, M. F., & Bó, G. A. (2012). Manipulation of follicle development to ensure optimal oocyte quality and conception rates in cattle. Reproduction in Domestic Animals, 47(4), 134–141. DOI: 10.1111/j.1439-0531.2012.02067.x

Baruselli, P. S. (2019). Avaliação de mercado de IATF no Brasil. Boletim Eletrônico do Departamento de Reprodução Animal/FMVZ/USP, 1. ed. Disponivel em: http://vra.fmvz.usp.br/boletim-eletronico-vra/. Acesso em: 01 Out. 2019.

Cardoso, D. (2007). Inseminação Artificial em Tempo Fixo: Uma biotecnologia a serviço do empresário rural. Pesquisa & Tecnologia, 4(1) Jan-Jun.

Carvalho, J. B. P., Carvalho, N. A. T., Reis, E. L., Nichi, M., Soares, J. G., & Baruselli, P. S. (2017). Efeito da prostaglandina f2? no início do protocolo de sincronização da ovulação em novilhas leiteiras Bos indicus, Bos taurus E Bos indicus x Bos taurus. Boletim de indústria animal, 74(2) 122-134. Disponivel em http://www.iz.sp.gov.br/pdfsbia/1500314591.pdf. DOI: 10.17523/bia.v74n2p122

Castro, N. A., Moreira, E. M., Andrade, J., Neves, P. M. A., Lemos, I. C., Souza, V. L., Nunes, V. R. R., Silva, R. R., Silva, G. M., Schneider, A., & Pfeifer, L. F. M. (2018). Perfil de liberação de LH circulante após tratamento com prostaglandina F2alfa em vacas ovariectomizadas. Anais da XXXII Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões (p.37). Florianópolis, SC, Brasil.

Fike, K. E., Day, M. L., Inskeep, E. K., Kinder, J. E., Lewis, P. E., Short, R. E., & Hafs, H. D. (1997). Estrus and Luteal Function in Suckled Beef Cows That Were Anestrous When Treated with an Intravaginal Device Containing Progesterone With or Without a Subsequent Injection of Estradiol Benzoate. Journal of Animal Science, 75(8), 2009-2015. DOI: 10.2527/1997.7582009x

Gimenes, L. U., Sá Filho, M. F., Carvalho, N. A. T., Torres-Junior, J. R., Souza, A. H., Madureira, E. H., Trinca, L. A., Sartorell, E. S., Barros, C. M., Carvalho, J. B., Mapletoft, R. J., & Baruselli, P. S. (2008). Follicle deviation and ovulatory capacity in Bos indicus heifers. Theriogenology¸ 69(7), 852-858. DOI: 10.1016/j/theriogenology.2008.01.001

Kasimanickam, R., Day, M. L., Rudolph, J. S., Hall, J. B., & Whittier, W. D. (2009). Two doses of prostaglandin improve pregnancy rates to timed-AI in a 5-day progesterone-based synchronization protocol in beef cows. Theriogenology, 71(5), 762-767. DOI: 10.1016/j.theriogenology.2008.09.049

Leonardi, C. E., Pfeifer, L. F., Rubin, M. I., Singh, J., Mapletoft, R. J. A., Pessoa, G. A., Bainy, A. M., & Silva, C. A. (2012). Prostaglandin F2? promotes ovulation in prepubertal heifers. Theriogenology, 78(7), 1578-1582. DOI: 10.1016/j.theriogenology.2012.06.030

Martinez, M. F., Adams, G. P., Bergfelt, D. R. Kastelic, J. P., & Mapletoft, R J. (1999). Effect of LH or GnRH on the dominant follicle of the first follicular wave in beef heifers. Animal Reproduction Science, 57(1-2), 23-33. DOI: 10.1016/s0378-4320(99)00057-3

Martinez, M. F., Kastelic, J. P., Adams, G. P., Cook, B., Olson, W. O., & Mapletoft, R. J. (2002). The use of progestins in regimens for fixed-time artificial insemination in beef cattle. Theriogenoly, 57(3), 1049-1059. DOI: 10.1016/S0093-691X(01)00682-3

Meneghetti, M., & Vasconcelos, J. L. M. (2008). Mês de parição, condição corporal e resposta a protocolos de inseminação artificial em tempo fixo em vacas de corte primíparas. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 60(4), 786-793.

Pereira, M. H., Wiltbank, M. C., Barbosa, L. F., Costa Júnior, W. M., Carvalho, M. A., & Vasconcelos, J. L. (2015). Effect of adding a gonadotropinreleasing- hormone treatment at the beginning and second prostaglandin F2? treatment at the end of an estradiol-based protocol for timed artificial insemination in lactating dairy cows during cool or hot seasons of the year. Journal of Dairy Science, 98(2), 947-959. DOI: 10.3168/jds.2014-8523

Peterson, C., Alkar, A., Smith, S., Kerr, S., Hall, J. B., Moore, D., & Kasimanickam, R., (2011). Effects of one versus two doses of prostaglandin F2alpha on AI pregnancy rates in a 5-day, progesterone-based, CO-Synch protocol in crossbred beef heifers. Theriogenology, 75(8), 1536-1542. DOI: 10.1016/j.theriogenology.2010.12.017

Pfeifer, L. F. M.; Siqueira, L. G.; Mapletoft, R. J,; Kastelic, J. P.; Adams, G. P.; Colazo, M. G.; Singh, J. (2009). Effects os exogenous progesterone and cloprostenol on ovarian follicular development and first ovulation in prepubertal heifers. Theriongenology, 72(8), 1054-1064. DOI: 10.1016/j.theriogenology.2009.06.022

Pfeifer, L. F. M., Leonardi, C. E. P., Castro, N. A., Viana, J. H. M., Siqueira, L. G. B., Castilho, E. M., Singh, J., Krusser, R. H., & Rubin, M. I. B. (2014). The use of PGF2a as ovulatory stimulus for timed artificial insemination in cattle. Theriogenology, 81(5), 689-695. DOI: 10.1016/j.theriogenology.2013.11.016

Randel, R. D., Lammoglia, M. A., Lewis, A. W., Neuendorff, D. A., & Guthrie, M. J. (1996). Exogenous PGF(2) alpha enhanced GnRH-induced LH release in postpartum cows. Theriogenology, 45(3), 643-654. DOI: 10.1016/0093-691X(95)00410-a

Ribeiro, E. S., Bisinotto, R. S., Favoreto, M. G., Martins, L. T., Cerri, R. L., Silvestre, F. T., Greco, L. F., Thatcher, W. W., & Santos, J. E. (2012). Fertility in dairy cows following presynchronization and administering twice the luteolytic dose of prostaglandin F2? as one or two injections in the 5-day timed artificial insemination protocol. Theriogenology, 78(2), 273-284. DOI: 10.1016/j.theriogenology.2012.01.012

Rodrigues, W. B.; Jara, J. P; Borges, J. C.; Oliveira, L. O. F.; Abreu, U. P. H.; Silva, K. C.; Anache, N. A.; Oliveira, A. B.; Cardoso Junior, C. T.; Nogueira, E. (2007). Ação da prostaglandina F2? como indutor de ovulação em vacas de corte submetidas a protocolos de IATF. Anais do XXII Colégio Brasileiro de Reprodução Animal (p.374). Santos, SP, Brasil.

Sartori, R., Fricke, P. M., Ferreira, J. C., Ginther, O. J., & Wiltbank, M. C. (2001). Follicular deviation and acquisition of ovulatory capacity in bovine follicles. Biology of Reproduction, 65(5), 1403-1409. DOI: 10.1095/biolreprod65.5.1403

Wiltbank, M. C.; Baez, G. M.; Cochrane, F., Barletta, R. V., Trayford, C. R., & Joseph, R. T. (2015). Effect of a second treatment prostaglandin F2 during the Ovsynch protocol on luteolysis and prengnancy in dairy cows. Journal of Dairy Science, 98(12), 8644-8654. DOI: 10.3168/jds.2015-9353

Young, I. M., Anderson, D. B., & Plenderleith, R. W. J. (1984). Increased conception rate in dairy cows after early postpartum administration of PGF2?. The Veterinary Record, 115(17), 429-431. DOI: 10.1136/vr.115.17.429




DOI: http://dx.doi.org/10.5433/1679-0359.2020v41n6p2669

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional.