Efeitos da adição de ácido docosahexaenoico e ?-tocoferol na qualidade do espermatozoide equino armazenados a 5°C
DOI:
https://doi.org/10.5433/1679-0359.2020v41n1p167Palavras-chave:
Antioxidante, DHA, Garanhão, Vitamina E.Resumo
A composição da membrana plasmática tem impacto na transição de fase do estado cristal líquido para o estado gel das células espermáticas submetidas à refrigeração. A incorporação de ácidos graxos poliinsaturados aumenta sua fluidez e pode contribuir para a motilidade espermática. O objetivo deste estudo foi comparar o efeito da adição de ácido docosahexaenóico (DHA) e ?-tocoferol (?-Toh) ao diluidor de refrigeração, isoladamente ou combinado, aos parâmetros do sêmen equino, submetidos à refrigeração por até 72 horas. Foram utilizados dois ejaculados de dez garanhões coletados com vagina artificial e avaliados quanto à motilidade, integridade da membrana plasmática, fragmentação da cromatina, atividade mitocondrial e peroxidação lipídica, de acordo com os seguintes tratamentos: C; DHA; a-Toh; DHA / a-Toh; EtOH 100: e EtOH 140 (correspondentes ao controle; 10 ng mL-1 de DHA; 2 mM de ?-Toh;: 10 ng mL-1 de DHA + 2 mM de ?-Toh; 100 µL de etanol e 140 µL de etanol, respectivamente). O tratamento com DHA mostrou maior motilidade (68,2 ± 12,3; p < 0,05) quando comparado aos grupos controle (62,1 ± 16,2), DHA/?-Toh (61,3 ± 12,7) e EtOH (58,1 ± 8,6). Na análise de peroxidação lipídica, o grupo controle mostrou 2.506,2 ± 796,4 ng de MDA 108 espermatozoides-1, sendo significativamente maior (p < 0,05) do que os grupos tratados com DHA (2.036,0 ± 687,0), ?-Toh (1.890,8 ± 749,5) e DHA / a-Toh (1.821,1 ± 627,2). Em conclusão, ?-Toh foi eficaz na diminuição da peroxidação lipídica de espermatozoide equino submetido ao resfriamento, e o DHA melhorou a motilidade espermática e, apesar de ser um ácido graxo poliinsaturado com alta suscetibilidade à peroxidação, reduziu a peroxidação lipídicaMétricas
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Referências
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Aurich, C. (2005). Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Animal Reproduction Science, 89(1-4), 65-75. doi: 10.1016/j.anireprosci.2005.06.025
Aurich, C. (2008). Recent advances in cooled-semen technology. Animal Reproduction Science, 107(3-4), 268-275. doi: 10.1016/j.anireprosci.2008.04.015
Ball, B. A., Medina, V., Gravance, C. G., & Baumber, J. (2001). Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C. Theriogenology, 56(4), 577-589. doi: 10.1016/S0093-691X(01)00590-8
Ball, B. A., & Vo, A. (2002). Detection of lipid peroxidation in equine spermatozoa based upon the lipophilic fluorescent dye C-11-BODIPY581/591. Journal of Andrology, 23(2), 259-269. doi: 10.1002/j.1939-4640.2002.tb02623.x
Ball, B. A. (2008). Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse. Animal Reproduction Science, 107(3-4), 257-267. doi: 10.1016/j.anireprosci.2008.04.014
Baumber, J., Ball, B. A., & Linfar, J. J. (2005). Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. American Journal of Veterinary Research, 66(5), 772-779. doi: 10.2460/ajvr.2005.66.772
Beletti, M. E., Costa, L. F., & Viana, M. P. (2004). Computational approach to characterization of bovine sperm chromatin alterations. Biotechnic and Histochemistry, 79(1), 17-23. doi: 10.1080/10520290410001700774
Brinsko, S. P., Varner, D. D., Love, C. C., Blanchard, T. L., Day, B. C., & Wilson, M. E. (2005). Effect of feeding DHA enriched nutraceutical on the quality of fresh, cooled and frozen stallion semen. Teriogenology, 63(5), 1519-1527. doi: 10.1016/j.theriogenology.2004.07.010
Castellini, C., Lattaioli, P., Moroni, M., & Minelli, A. (2000). Effect of seminal plasma on the characteristics and fertility of rabbit spermatozoa. Animal Reproduction Science, 63(3-4), 275-282. doi: 10.1016/S0378-4320(00)00181-0
Cerolini, S., Maldjian, A., Surai, P., & Noble, R. (2000). Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Animal Reproduction Science, 58(1-2), 99-111. doi: 10.1016/S0378-4320(99)00035-4
Connor, W. E., Lin, D. S., Wolf, D. P., & Alexander, M. (1998). Uneven distribution of desmosterol and docosahexaenoic acid in the heads and tails of monkey sperm. Journal of Lipid Research, 39(7), 1404-1411.
Dodaran, H. V., Zhandi, M., Sharafi, M., Nejati-Amiri, E., Nejati-Javaremi, A., Mohammadi-Sangcheshmeh, A., Shakeri, M. (2015). Effect of ethanol induced mild stress on post thawed bull sperm quality. Cryobiology, 71(1), 12-17. doi: 10.1016/j.cryobiol.2015.06.008
Evenson, D. P. & Jost, L. (2000). Sperm chromatin structure assay is useful for fertility assessment. Methods in Cell Science, 22(2-3), 169-189.
Franco, J. S. V., Chaveiro, A., Góis, A., & Silva, F. M. (2013). Effects of alfa tocopherol and ascorbic acid on equine semen quality after cryopreservation. Journal of Equine Veterinary Science, 33(10), 787-793. doi: 10.1016/j.jevs.2012.12.012
Gawrisch, K., Eldho, N. V., & Holte, L. L. (2003). The structure of DHA in phospholipid membranes. Lipids, 38(4), 445-452. doi: 10.1007/s11745-003-1082-0
Gibb, Z., Lambourne, S. R., & Aitken, R. J. (2014). The paradoxical relationship between stallion fertility and oxidative stress. Biology of Reproduction, 91(3), 1-10. doi: 10.1095/biolreprod.114.118539
Giraud, M. N., Motta, C., Boucher, D., & Grizard, G. (2000). Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Human Reproduction, 15(10), 2160-2164. doi: 10.1093/humrep/15.10.2160
Green, P., Glozman, S., & Yavin, E. (2001a). Ethyl docosahexaenoate-associated decrease in fetal brain lipid peroxide production is mediated by activation of prostanoid and nitric oxide pathways. Biochimica et Biophysica Acta, 1531(1-2), 156-164. doi: 10.1016/S1388-1981(01)00101-9
Green, P., Glozman, S., Weiner, L., & Yavin, E. (2001b). Enhanced free radical scavenging and decreased lipid peroxidation in the rat fetal brain after treatment with ethyl docosahexaenoate. Biochimica et Biophysica Acta, 1532(3), 203-212. doi: 10.1016/S1388-1981(01)00132-9
Harrison, R. A. P., & Vickers, S. E. (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility, 88(1), 343-352. doi: 10.1530/jrf.0.0880343
Howard, A. C., McNeil, A. K. & McNeil, P. L. (2011). Promotion of plasma membrane repair by vitamin E. Nature Communications, 20, 1-8. doi: 10.1038/ncomms1594
Hrudka, F. (1987). Cytochemical and ultracytochemical demonstration of cytochrome c oxidase in spermatozoa and dynamics of its changes accompanying ageing or induced by stress. International Journal of Andrology, 10, 809-828. doi: 10.1111/j.1365-2605.1987.tb00385.x
Kaeoket, K., Sang-Urai, P., Thamniyom, A., Chanapiwat, P., & Techakumphu, M. (2010). Effect of docosahexaenoic acid on quality of cryopreserved boar semen in different breeds. Reproduction in Domestic Animals, 45(3), 458-463. doi: 10.1111/j.1439-0531.2008.01239.x
Kaka, A., Haron, W., Yusoff, R., Yimer, N., Khumran, A. M., Sarsaifi, K., Ebrahimi, M. (2015). Effect of docosahexanoic acid on quality of frozen-thawed bull semen in BioXcell extender. Reproduction, Fertility and Development, 29(3), 490-495. doi: 10.1071/RD15089
Keshtgar, S., Fanaei, H., Bahmanpour, S., Azad, F., Ghannadi, A., & Kazeroni, M. (2012). In vitro effects of a-tocopherol on teratozoospermic semen samples. Andrologia, 44(1), 721-727. doi: 10.1111/j.1439-0272.2011.01256.x
Linfor, J. J., & Meyers, S. A. (2002). Detection of DNA damage in response to cooling injury in equine spermatozoa using single cell gel electrophoresis. Journal of Andrology, 23(1), 107-113. doi: 10.1002/j.1939-4640.2002.tb02603.x
Losano, J. D. A., Angrimani, D. S. R., Rui, B. R., Bicudo, L. C., Dalmazzo, A., Silva, B. C. S., Nichi, M. (2018). The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote, 26(3), 199-206. doi: 10.1017/S0967199418000096
Love, C. C. (2005). The sperm chromatin structure assay: a review of clinical applications. Animal Reproduction Science, 89(1-4), 39-45. doi: 10.1016/j.anireprosci.2005.06.019
Nasiri, A. H., Towhidi, A., Zeinoaldini, S. (2012). Combined effect of DHA and alfa tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition. Andrologia, 44(1), 550-555. doi: 10.1111/j.1439-0272.2011.01225.x
Naves, C. S., Beletti, M. E., Duarte, M. B., Vieira, R. C., Diniz, E. G., & Jacomini, J. O. (2004). Avaliação da cromatina espermática em equinos com azul de toluidina e acridine orange. Bioscience Journal, 20(3), 117-124.
Neill, A. R., & Masters, C. J. (1972). Metabolism of fatty acids by bovine spermatozoa. Biochemical Journal, 127(2), 375-385. doi: 10.1042/bj1270375
Nichi, M., Goovaerts, I. G. F., Cortada, C. N. M., Barnabe, V. H., De Clercq, J. B. P., & Bols, P. E. J. (2007). Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymis stored at 4 and 34°C. Theriogenology, 67(2), 334-340. doi: 10.1016/j.theriogenology.2006.08.002
Nunes, D. B., Zorzatto, J. R., Costa e Silva, E. V., & Zúccari, C. E. S. N. (2008). Efficiency of short-term storage of equine semen in a simple design cooling system. Animal Reproduction Science, 104(2-4), 434-439. doi: 10.1016/j.anireprosci.2007.06.022
Sampaio, I. B. M. (2007). Estatística aplicada à experimentação animal (3a ed.) Belo Horizonte: Fundação de Ensino e Pesquisa em Medicina Veterinária e Zootecnia.
Sanocka, D., & Kurpisz, M. (2004). Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology, 2(12), 1-7. doi: 10.1186/1477-7827-2-12
Silva, D. M., Holden, S. A., Lyons, A., Souza, J. C., & Fair, S. (2017). In vitro addition of docosahexaenoic acid improves the quality of cooled but not frozen thawed stallion semen. Reproduction, Fertility and Development, 29(10), 2021-2027. doi: 10.1071/RD16473
Sindelar, P. J., Guan, Z., Dallner, G., & Ernster, L. (1999). The protective role of plasmalogens in iron induced lipid peroxidation. Free Radical Biology and Medicine, 26(3-4), 318-324. doi: 10.1016/s0891-5849(98)00221-4
Stillwell, W., & Wassall, S. R. (2003). Docosahexaenoic acid: membrane properties of a unique fatty acid. Chemistry and Physics of Lipids, 126(1), 1-27. doi: 10.1016/S0009-3084(03)00101-4
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2020-01-10
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Sampaio, B. F. B., Nogueira, B. G., Souza, M. I. L., Costa-e-Silva, E. V. da, & Neto Zúccari, C. E. S. (2020). Efeitos da adição de ácido docosahexaenoico e ?-tocoferol na qualidade do espermatozoide equino armazenados a 5°C. Semina: Ciências Agrárias, 41(1), 167–180. https://doi.org/10.5433/1679-0359.2020v41n1p167
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