Supplementation of cows with linseed: effects on antral follicle count, oocyte quality, and nutritional and plasma metabolite parameters

Christopher Junior Tavares Cardoso, Ana Caroline Bini de Lima, Wilian Aparecido Leite da Silva, Mirela Brochado Souza-Cáceres, Daniela Moraes Pereira, Marcus Vinicius Morais de Oliveira, Ériklis Nogueira, Fabiana de Andrade Melo-Sterza

Abstract


Linseed is distinguished by its composition of fatty acids, which are rich in unsaturated acids, and by the slow release of oil in the rumen, consequently providing a greater amount of lipids of interest for intestinal absorption. The aim of this study was to evaluate the effect of linseed supplementation on the antral follicle count (AFC) and oocyte quality of Girolando cows, as well as their digestive parameters and plasma metabolite. Twelve Girolando cows were used in the study and were randomly distributed in two experimental groups: control (CTL, n = 6) and linseed (LINS, n = 6). During the 126 days of supplementation, seven transvaginal ultrasound-guided ovum pick-up (OPU) sessions were performed at intervals of 21 days. AFC was performed in each OPU session. The cows fed with linseed showed no significant difference in the dry matter coefficient of digestibility (63.11 and 62.88), crude protein (62.7 and 55.26), neutral detergent fiber (NDF; 75.36 and 77.15), or acid detergent fiber (ADF; 72.45 and 74.77) for LINS and CTL, respectively. On the contrary, a higher level of ethereal extract was observed for LINS (69.31 vs. 40.7). There was no effect (p > 0.05) of lipid supplementation on plasma metabolite between groups and OPU sessions. There was no difference (p > 0.05) in the mean number of antral follicles (CTL: 31.14 ± 1.97; LINS: 25.52 ± 2.68), cumulus oocyte complexes recovered (CTL: 6.45 ± 1.66; LINS: 5.28 ± 1.18), or oocyte quality (CTL: 60.48% ± 8.46; LINS: 64.54% ± 7.77). The supplementation of 800g of linseed in the diet of Girolando cows did not alter the apparent digestibility of nutrients, AFC, or quality of oocytes obtained by OPU.

Keywords


Polyunsaturated fatty acid; Reproduction; Digestibility.

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References


ACCORSI, M. F.; LEAO, B. C.; ROCHA-FRIGONI, N. A.; PERRI, S. H.; MINGOTI, G. Z. Reduction in cytoplasmic lipid content in bovine embryos cultured in vitro with linoleic acid in semi-defined medium is correlated with increases in cryotolerance. Zygote, Cambridge, v. 24, n. 4, p. 485-494, 2016. DOI: 10.1017/S 0967199415000428

BILBY, T. R.; BLOCK, J.; AMARAL, B. C.; SA FILHO, O.; SILVESTRE, F. T.; HANSEN, P. J.; STAPLES, C. R.; THATCHER, W. W. Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in summer. Journal of Dairy Science, Lancaster, v. 89, n. 10, p. 3891-3903, 2006. DOI: 10.3168/jds.S0022-0302(06)72432-8

BOLAND, M. P.; LONERGAN, P.; O'CALLAGHAN, D. Effect of nutrition on endocrine parameters, ovarian physiology, and oocyte and embryo development. Theriogenology, Stoneham, v. 55, n. 6, p. 1323-1340, 2001. DOI: 10.1016/S0093-691X(01)00485-X

CAMPBELL, A. G. Grazed pasture parameters. I. Pasture dry-matter production and availability in a stocking rate and grazing management experiment with dairy cows. Journal of Agricultural Science, Cambridge, v. 67, n. 2, p. 199-210, 1966. DOI: 10.1017/S0021859600068283

CARDOSO, C. J. T.; OLIVEIRA JUNIOR, J. S.; KISCHEL, H.; SILVA, W. A. L.; ARRUDA, E. D. S.; SOUZA-CÁCERES, M. B.; OLIVEIRA, F. A. M.; NOGUEIRA, É.; NOGUEIRA, G. P.; MELO-STERZA, F. A. Anti-Müllerian hormone (AMH) as a predictor of antral follicle population in heifers. Animal Reproduction, Belo Horizonte, v. 15, n. 1, p. 12-16, 2018a. DOI: 10.21451/1984-3143-2017-AR887

CARDOSO, C. J. T.; LIMA, A. C. B.; NOGUEIRA, É.; OLIVEIRA, M. V. M.; MELO-STERZA, F. A. Performance and digestive characteristics in dairy cattle maintained under grazing regime and supplemented with linseed. Semina: Ciências Agrárias, Londrina, v. 39, n. 4, p. 1805-1818, 2018b. DOI: 10.5433/1679-0359.2018v39n4p1805

CAVALIERI, F. B.; SANTOS, G. T.; MATSUSHITA, M.; PETIT, H. V.; RIGOLON, L. P.; SILVA, D.; HORST, J. A.; CAPOVILLA, L. C.; RAMOS, F. S. Milk production and milk composition of dairy cows fed Lac100® or whole flaxseed. Canadian Journal of Animal Science, Ottawa, v. 85, n. 3, p. 413-416, 2005. DOI: 10.4141/A04-088

CERRI, R. L.; JUCHEM, S. O.; CHEBEL, R. C.; RUTIGLIANO, H. M.; BRUNO, R. G.; GALVAO, K. N.; SANTOS, J. E. Effect of fat source differing in fatty acid profile on metabolic parameters, fertilization, and embryo quality in high-producing dairy cows. Journal of Dairy Science, Lancaster, v. 92, n. 4, p. 1520-1531, 2009. DOI: 10.3168/jds.2008-1614

CHILDS, S.; CARTER, F.; LYNCH, C. O.; SREENAN, J. M.; LONERGAN, P.; HENNESSY, A. A.; KENNY, D. A. Embryo yield and quality following dietary supplementation of beef heifers with n-3 polyunsaturated fatty acids (PUFA). Theriogenology, Stoneham, v. 70, n. 6, p. 992-1003, 2008. DOI: 10.1016/j.theriogenology.2008.06.008

CLIPES, R. C.; COELHO da SILVA, J. F.; DETMANN, E.; VASQUEZ, H. M.; SCOLFOROI, L.; LOMBARDI, C. T. Avaliação de métodos de amostragem em pastagens de capim elefante (Pennisetum purpureum, Schum) e capim-mombaça (Panicum maximum, Jacq) sob pastejo rotacionado. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 57, n. 1, p. 120-127, 2005. DOI: 10.1590/S0102-09352005000100016

COCHRAN, R. C.; ADAMS, D. C.; WALLACE, J. D.; GALYEAN, M. L. Predicting digestibility of different diets with internal markers: evaluation of four potential markers. Journal of Animal Science, Lancaster, v. 63, n. 5, p. 1476-1483, 1986. DOI: 10.2527/jas1986.6351476x

ECKERSALL, P. D. Proteins, proteomics, and thedysproteinemias. In: KANEKO, J. J.; HARVEY, J. W.; BRUSS, M. L. (Ed.). Clinical biochemistry of domestic animals. 6th ed. San Diego: Academic Press, 2008. p. 117-155.

FOULADI-NASHTA, A. A.; WONNACOTT, K. E.; GUTIERREZ, C. G.; GONG, J. G.; SINCLAIR, K. D.; GARNSWORTHY, P. C.; WEBB, R. Oocyte quality in lactating dairy cows fed on high levels of n-3 and n-6 fatty acids. Reproduction, Cambridge, v. 138, n. 5, p. 771-781, 2009. DOI: 10.1530/REP-08-0391

GAGLIOSTRO, G.; CHILLIARD, Y. Duodenal rapeseed oil infusion in early and midlactation cows. 4. In vivo and in vitro adipose tissue lipolytic responses. Journal of Dairy Science,Lancaster, v. 74, n. 6, p. 1830-1843, 1991. DOI: 10.3168/jds.S0022-0302(91)78348-3

GUARDIEIRO, M. M.; BASTOS, M. R.; MOURÃO, G. B.; CARRIJO, L. H. D.; MELO, E. O.; RUMPF, R.; SARTORI, R. Função ovariana de novilhas Nelore alimentadas com dieta suplementada com gordura protegida ruminal. Pesquisa Agropecuária Brasileira, Brasília, v. 45, n. 4, p. 408-414, 2011. DOI: 10.1590/S0100-204X2010000400009

HAWKINS, D. E.; NISWENDER, K. D.; OSS, G. M.; MOELLER, C. L.; ODDE, K. G.; SAWYER, H. R.; NISWENDER, G. D. An increase in serum lipids increases luteal lipid content and alters the disappearance rate of progesterone in cows. Journal of Animal Science, Lancaster, v. 73, n. 2, p. 541-545, 1995. DOI: 10.2527/1995.732541x

HOUGHTON, P.; LEMENAGER, R.; HENDRIX, K.; MOSS, G.; STEWART, T. Effects of body composition, pre-and postpartum energy intake and stage of production of energy utilization by beef cows. Journal of Animal Science, Lancaster, v. 68, n. 5, p. 1447-1456, 1990. DOI: 10.2527/1990.6851447x

JENKINS, T. C.; JENNY, B. F. Effect of hydrogenated fat on feed intake, nutrient digestion, and lactation performance of dairy cows. Journal of Dairy Science, Lancaster, v. 72, n. 9, p. 2316-2324, 1989. DOI: 10.3168/jds.S0022-0302(89)79363-2

KIM, J. Y.; KINOSHITA, M.; OHNISHI, M.; AND FUKUI, Y. Lipid and fatty acid analysis of fresh and frozen thawed immature and in vitro-matured bovine oocytes. Reproduction, v. 122, n. 1, p. 131-138, 2001. DOI:10.1530/REP.0.1220131

LEÃO, B. C.; ROCHA-FRIGONI, N. A.; CABRAL, E. C.; FRANCO, M. F.; FERREIRA, C. R.; EBERLIN, M. N.; FILGUEIRAS, P. R.; MINGOTI, G. Z. Membrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos. Zygote, Cambridge, v. 23, n. 5, p. 732-741, 2015. DOI: 10.1017/S0967199414000380

LOPES, C. N.; SCARPA, A. B.; CAPPELLOZZA, B. I.; COOKE, R. F.; VASCONCELOS, J. L. Effects of rumen-protected polyunsaturated fatty acid supplementation on reproductive performance of Bos indicus beef cows. Journal of Animal Science, Lancaster, v. 87, n. 12, p. 3935-3943, 2009. DOI: 10.2527/jas.2009-2201

MAREI, W. F.; WATHES, D. C.; FOULADI-NASHTA, A. A. The effect of linolenic acid on bovine oocyte maturation and development. Biology of Reproduction, New York, v. 81, n. 6, p. 1064-1072, 2009. DOI: 10.1095/biolreprod.109.076851

MATTOS, R.; STAPLES, C. R.; THATCHER, W. W. Effects of dietary fatty acids on reproduction in ruminants. Reviews of Reproduction, Madrid, v. 5, n. 1, p. 38-45, 2000.

MIHM, M.; CROWE, M. A.; KNIGHT, P. G.; AUSTIN, E. J. Follicle wave growth in cattle. Reproduction in Domestic Animals, Malden, v. 37, n. 4, p. 191-200, 2002. DOI: 10.1046/j.1439-0531.2002.00371.x

MONNIAUX, D.; HUET, C.; BESNARD, N.; CLEMENT, F.; BOSC, M.; PISSELET, C.; MONGET, P.; MARIANA, J. C. Follicular growth and ovarian dynamics in mammals. Journal of Reproduction and Fertility Supplementary, Cambridge, v. 51, p. 3-23, 1997.

NATIONAL RESEARCH COUNCIL - NRC. Nutrient requirements of dairy cattle. 7th ed. Washington: Academic Press, 2001. 381 p.

NOGUEIRA, É.; SILVA, A. S. D.; AMARAL, T. B.; ÍTAVO, L. C. V.; DIAS, A. M.; MINGOTI, G. Z. Follicular dynamics and production of oocytes in young Nellore heifers with energetic supplementation. Revista Brasileira de Zootecnia, Viçosa, MG, v. 41, n. 9, p. 2012-2017, 2012. DOI: 10.1590/S1516-35982012000900007

NUNES, A. A.; FAVARO, S. P.; GALVANI, F.; MIRANDA, C. H. B. Good practices of harvest and processing provide high quality Macauba pulp oil. European Journal of Lipid Science and Technology, Weinheim, v. 117, n. 12, p. 2036-2043, 2015. DOI: 10.1002/ejlt.201400577

PALMQUIST, D. L.; JENKINS, T. C. Fat in lactation rations: review. Journal of Dairy Science, Lancaster, v. 63, n. 1, p. 1-14, 1980. DOI: 10.3168/jds.S0022-0302(80)82881-5

PETIT, H. V. Digestion, milk production, milk composition, and blood composition of dairy cows fed whole flaxseed. Journal of Dairy Science, Lancaster, v. 85, n. 6, p. 1482-1490, 2002. DOI: 10.3168/jds.S0022-0302(02)74217-3

PETIT, H. V.; DEWHURST, R. J.; SCOLLAN, N. D.; PROULX, J. G.; KHALID, M.; HARESIGN, W.; TWAGIRAMUNGU, H.; MANN, G. E. Milk production and composition, ovarian function, and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science, Lancaster, v. 85, n. 4, p. 889-899, 2002. DOI: 10.3168/jds.S0022-0302(02)74147-7

PONTER, A. A.; GUYADER-JOLY, C.; NUTTINCK, F.; GRIMARD, B.; HUMBLOT, P. Oocyte and embryo production and quality after OPU-IVF in dairy heifers given diets varying in their n-6/n-3 fatty acid ratio. Theriogenology, Stoneham, v. 78, n. 3, p. 632-645, 2012. DOI: 0.1016/j.theriogenology.2012.03.009

RYAN, D. P.; BAO, B.; GRIFFITH, M. K.; WILLIAMS, G. L. Metabolic and luteal sequelae to heightened dietary fat intake in undernourished, anestrous beef cows induced to ovulate. Journal of Animal Science, Lancaster, v. 73, n. 7, p. 2086-2093, 1995. DOI: 10.2527/1995.7372086x

SANTOS, J. E.; CERRI, R. L.; SARTORI, R. Nutritional management of the donor cow. Theriogenology, Stoneham, v. 69, n. 1, p. 88-97, 2008. DOI: 10.1016/j.theriogenology.2007.09.010

SARTORI, R.; GIMENES, L. U.; MONTEIRO JUNIOR, P. L.; MELO, L. F.; BARUSELLI, P. S.; BASTOS, M. R. Metabolic and endocrine differences between Bos taurus and Bos indicus females that impact the interaction of nutrition with reproduction. Theriogenology, Stoneham, v. 86, n. 1, p. 32-40, 2016. DOI: 10.1016/j.theriogenology.2016.04.016

SILVA, D.; QUEIROZ, A. C. Análise de alimentos: métodos químicos e biológicos. Viçosa, MG: UFV, Impr. Univ., 1981. 166 p.

STAPLES, C. R.; BURKE, J. M.; THATCHER, W. W. Influence of supplemental fats on reproductive tissues and performance of lactating cows. Journal of Dairy Science, Lancaster, v. 81, n. 3, p. 856-871, 1998. DOI: 10.3168/jds.S0022-0302(98)75644-9

THANGAVELU, G.; COLAZO, M. G.; AMBROSE, D. J.; OBA, M.; OKINE, E. K.; DYCK, M. K. Diets enriched in unsaturated fatty acids enhance early embryonic development in lactating Holstein cows. Theriogenology, Stoneham, v. 68, n. 7, p. 949-957, 2007. DOI: 10.1016/j.theriogenology.2007.07.002

THOMAS, M. G.; WILLIAMS, G. L. Metabolic hormone secretion and FSH-induced superovulatory responses of beef heifers fed dietary fat supplements containing predominantly saturated or polyunsaturated fatty acids. Theriogenology, Stoneham, v. 45, n. 2, p. 451-458, 1996. DOI: 10.1016/0093-691X(95)00381-H

VAN SOEST, P. J.; ROBERTSON, J. B.; LEWIS, B. A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, Lancaster, v. 74, n. 10, p. 3583-3597, 1991. DOI: 10.3168/jds.S0022-0302(91)78551-2

WEBB, R.; GARNSWORTHY, P. C.; GONG, J. G.; ARMSTRONG, D. G. Control of follicular growth: local interactions and nutritional influences. Journal of Animal Science, Lancaster, v. 82, p. E63-E74, 2004. Supplement 13. DOI: 10.2527/2004.8213_supplE63x

WU, Z.; PALMQUIST, D. L. Synthesis and biohydrogenation of fatty acids by ruminal microorganisms in vitro. Journal of Dairy Science, Lancaster, v. 74, n. 9, p. 3035-3046, 1991. DOI: 10.3168/jds.S0022-0302(91 )78489-0

ZERON, Y.; SKLAN, D.; ARAV, A. Effect of polyunsaturated fatty acid supplementation on biophysical parameters and chilling sensitivity of ewe oocytes. Molecular Reproduction and Development, New York, v. 61, n. 2, p. 271-278, 2002. DOI: 10.1002/mrd.1156




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n6p2637

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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