Purification of amyloglucosidase from Aspergillus niger

Ana Paula Manera, Simone Meinhardt, Susana Juliana Kalil

Abstract


In this work, test were carried out to determine the best conditions for the purification of amyloglucosidase from Aspergillus niger NRRL 3122, using a fixed-bed ion exchange chromatography column. The tests were conducted in a purification column containing anion resin DEAE-cellulose. To maximize the purification conditions, were used as variable parameters feeding speed (46 and 8 cm/h) and operating temperature (25 and 10ºC). The best result was observed when a feeding speed of 8 cm/h was employed at a temperature of 10ºC, resulting in a purification factor of 9.9-fold and a recovery of 47.6%.


Keywords


DEAE-cellulose; Enzyme; Temperature.



DOI: http://dx.doi.org/10.5433/1679-0359.2011v32n2p651

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional