Effect of the use of golden mussel flour contaminated with lead as a source of calcium on the performance of broilers

Lucas Wachholz, Ricardo Vianna Nunes, Affonso Celso Gonçalves Junior, Cinthia Eyng, Leandro Dalcin Castilha, Edinan Hagdon Cirilo, Jomara Broch, Cleison de Souza

Abstract


The objective of this study was to evaluate the use of lead contaminated golden mussel flour (GMF) as a source of calcium for broiler chickens from 14 to 42 days of age and to verify the probable effects on performance, tissue contamination, blood parameters, Pb digestibility, and bone quality. The experiment was conducted in a completely randomized design (DIC), with four levels of Pb contamination (71.33, 147.55, 223.78, and 300 mg kg-1 Pb) in GMF, with five replicates and three birds per experimental unit. Sixty-four-day-old Cobb male broilers housed in metabolism cages were used. GMF was added to rations instead of calcitic limestone. The zootechnical variables were evaluated: feed intake, weight gain, and feed conversion, as well as the digestibility of nutrients. In addition, the serum biochemistry of the birds transaminases, total bilirubin, calcium, and phosphate was studied and the concentration of lead in the tissues and the bone quality of the birds was determined. The tibia was collected for evaluation of bone quality. The levels of Pb contamination in the golden mussel did not change the performance of the birds (P ? 0.05) or the Pb levels in the muscle Pectoralis major, skin, liver, feather, bones, and serum, or the serum levels of ALT, GGT, and total bilirubin, however, there was an effect of Pb levels (P < 0.01) on AST concentrations. Pb levels altered the values (P < 0.01) of the apparent digestible Pb and the coefficient of apparent digestibility of Pb, with an increasing linear effect. There was no effect (P ? 0.05) on bone stiffness, however flexibility and bone strength were altered (P < 0.05). Serum Ca and P concentrations did not show changes (P ? 0.05) due to the use of Pb levels in GMF. In this study, it can be observed that the use of GMF with Pb up to 300 mg kg-1 as a source of Ca in broiler diets does not alter performance, however the use of GMF contaminated with Pb above 73.33 mg kg-1 promotes contamination of the tissues of the animals with Pb, making it impossible to consume the birds. It was concluded that the increase in Pb concentrations in GMF increases the digestibility and metabolism of this metal and causes an increase in the production of AST by the liver. Pb levels in GMF above 214.11 mg kg-1 impair bone strength and flexibility.

Keywords


Food; Poultry; Pollutants; Digestibility; Alternative ingredient; Transaminases.

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References


AGENCY FOR TOXIC SUBSTANCES AND DISEASE REGISTRY - ATSDR. Toxicological profile for lead. Atlanta, GA: Department of Health and Human Services, 2007. 582 p.

ALMEIDA, M. E. F.; TAGLIASSACHI, P. M.; TAVARES, R. O.; LUJAN, D. L. B. Reversão da osteoporose: uma realidade possível. Journal of Health & Biological Sciences, Fortaleza, v. 3, n. 4, p. 245-248, 2015. DOI: 10.12662/2317-3076jhbs.v3i4.191.p245-248.2015

ANTONIO, L. L. C. P. S.; CAPILÉ, H. E.; DUARTE, R. Mexilhão dourado (Limnoperna fortunei) como bioindicador aquático. Pesquisa & Educação a Distância, São Gonçalo, v. 4, n. 8, 2017, p. 1-14.

ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS - AOAC. Official methods of analysis of the Association Analytical Chemists. 18. ed. Gaithersburg, Maryland: AOAC, 2005.

BAYERLE, D. F.; NUNES, R. V.; GONÇALVES JUNIOR, A. C.; WACHHOLZ, L.; SCHERER, C.; SILVA, I. M.; BRUXEL, T. M. O.; VARGAS JUNIOR, J. G. Golden mussel (Limnoperna fortunei) in feed for broiler chicks using tannin as a sequestrant of toxic metals. Semina: Ciências Agrárias, Londrina, v. 38, n. 2, p.843-854, 2017. DOI: 10.5433/1679-0359.2017v38n2p843

BRASIL. Decreto nº 55.871 de 26 de março de 1965. Modifica o Decreto nº 50.040, de 24 de janeiro de 1961, referente a normas reguladoras do emprego de aditivos para alimentos, alterado pelo Decreto nº 691, de 13 de março de 1962. Diário Oficial [da] União, Brasília, 9 abr. 1965. Seção 1, pt. 1, p. 3611.

BORSA, A.; KOHAYAGAWA, A.; BORETTI, L. P.; SAITO, M. E.; KUIBIDA, K. Níveis séricos de enzimas de função hepática em frangos de corte de criação industrial clinicamente saudáveis. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 58, n. 4, p.675-677, 2006. DOI: 10.1590/S0102-09352006000400035

CANZI, C.; FIALHO, N.; BUENO, G. W. Monitoramento e ocorrência do mexilhão dourado (Limnoperna fortunei) na hidrelétrica da Itaipu binacional, Paraná (BR). Revista Ibero-Americana de Ciências Ambientais, Aracaju, v. 5, n. 2, p. 117-122, 2014. DOI: 10.6008/SPC2179-6858.2014.002.0011

CHIU, T. Y.; TENG, H. C.; HUANG, P. C.; KAO, F. J.; YANG, D. M. Dominant role of Orai1 with STIM1 on the cytosolic entry and cytotoxicity of lead ions. Toxicologycal Sciences, Reston, v. 110, n. 2, p.353-362, 2009. DOI: 10.1093/toxsci/kfp099

COUTINHO, F. M. B; GOMES, A. S.; TEIXEIRA, V. G.; Resinas poliméricas para separação e pré-concentração de chumbo. Química Nova, São Paulo, v. 27, n. 2, p. 754-762, 2004. DOI: 10.1590/S0100-40422004000500015

CURREY, J. D. The many adaptation of bone. Journal of Biomechanics, Rotterdam, v. 36, n. 10, p. 1487-1495, 2003.

EUROPEAN COMMISSION REGULATION. Directive n 2005/87/Ce of Commission of December. Official Journal of European Union, l318/19-l318/24, 2005.

GONÇALVES, F. G.; ZANINI, S. R.: GUERRA, A. F. Q. G.; GONÇALVES, E. P.; COLNAGO, G. L.; FEITOSA, M. L. Utilização de pimenta rosa como um aditivo natural na dieta sobre o desempenho de frangos de corte. Revista Brasileira de Saúde e Produção Animal, Salvador, v. 15, n. 1, p. 28-38, 2014. DOI: 10.1590/S1519-99402014000100007

OMOBOWALE, T. O.; OYAGBEMI, A. A.; AKINRINDE, A. S.; SABA, A. B.; DARAMOLA, O. T.; OGUNPOLU, B. S.; OLOPADE, J. O. Failure of recovery fron lead induced hepatoxicity and disruption of erythrocute antioxidant defence system in winstar rats. Environmental Toxicology and Pharmacology, v. 37, n. 3, p. 1202-1211, 2014. DOI: 10.1016/j.etap.2014.03.002

OLIVEIRA, A. F. G.; BRUNO, L. D. G.; MARTINS, E. N.; DE MORAES GARCIA, E. R.; MONTEIRO, A. C.; PAULA LEITE, M. C. de; POZZA, P. C.; SANGALI, C. P. Effect of stocking density and genetic group on mineral composition and development of long bones of broilers. Semina: Ciências Agrárias, v. 35, n. 2, p. 1023-1034, 2014. DOI: 10.5433/1679-0359.2014v35n2p1023

SAKOMURA, N. K.; ROSTAGNO, H. S. Métodos de pesquisa em nutrição de monogástricos. Jaboticabal: FUNEP, 2016. 262 p.

SAKOMURA, N. K.; VILAR DA SILVA, J. H.; PERAZZOCOSTA, F. G.; FERNANDES, J. B. K.; HAUSCHILD, E. U. Nutrição de não-ruminantes. Jaboticabal: FUNEP, 2014. 678 p.

SAS INSTITUTE INC - SAS. SAS University edition: installation guide for windows. Cary, NC: SAS Institute, 2018. 24 p.

SILVA, P. R. L.; FREITAS NETO O. C.; LAURENTIZ A. C.; JUNQUEIRA O. M.; FAGLIARI J. J. Blood serum components and serum protein test of Hybro-PG broilers of different ages. Revista Brasileira de Ciência Avícola, Campinas, v. 9, n. 4, p. 229-232, 2007. DOI: 10.1590/S1516-635X2007000400004

SIMKISS, K. Amorphous minerals in biology. Bulletin Institute Oceanographic, San Diego, v. 14, n. especial, p. 49-54, 1993.

SOUZA, C. S.; VIEITES, F. M. Vitamin D3 and its metabolities for broilers. Archivos de Zootecnia, Córdoba, v. 63, n. 241, p. 11-24, 2014. DOI: 10.5713/ajas.2012.12455

SOUZA, V. F.; NOBREGA, M. A. S.; PONTES, M. S. Musgos como bioindicadores de metais pesados no ambiente. Acta Biomedica Brasiliensia, Itaperuna, v. 8, n. 2, p. 13-22, 2017. DOI: 10.18571/acbm.136

THRALL, M. A. Hematologia e bioquímica clínica veterinária. São Paulo: Editora Roca, 2015. 688p.

WACHHOLZ, L.; NUNES, R.V.; BROCH, J.; SOUZA, C. Possibilidade do uso de mexilhão dourado contaminado com metais tóxicos em dietas para frangos de corte. Revista Colombiana de Ciência Animal, Sincelejo, v. 9, n .2, p. 227-235, 2017. DOI: 10.24188/recia.v9.n2.2017.562

WANG, H.; WANG, Z.; JIAO, P.; ZHOU, X.; YANG, D.; YOUNG, Z. W.; WANG, L. Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead. Toxicology, v. 333, n. 3, p. 137-146, 2015. DOI: 10.1016/j.tox.2015.04.015




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n6p2783

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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