Comparison of two methods for the quantification of gastrointestinal nematode infective larvae from pasture

Marina Lais Sabião de Toledo Piza, Fabiana Alves de Almeida, Cristiano Magalhães Pariz, Ciniro Costa, Alessandro Francisco Talamini do Amarante

Abstract


The economic losses caused by gastrointestinal nematodes are one of the biggest obstacles in the small ruminants production. Understanding the population dynamics of the infective larvae (L3) in the pasture is the key point to develop control programs, and reliable results depend on the used methodology to quantify L3 numbers. The use of the sampling directly from the pasture appears as a viable option, since it is not required the use of animals with an esophageal fistula or tracer animals, decreasing the costs involved in the study. Therefore, the present project, which had as objective evaluate the efficiency of two collection methods for quantification of L3 in the pasture, utilized 64 lambs (n = 16) allocated to four integrated crop-livestock systems (treatments) with 12 paddocks each. Pasture samples were collected every nine days. The W method consists in traversing the area in the form of a W and again an inverted W, forage samples being collected every 10 steps, and the Square method, in tossing a 0.16 m2 square to four random points within the area, the forage within the square being collected after each toss. After the forage samples had been processed, the L3 were recovered and identified. Cohen’s Kappa coefficient (k) was determined. The W-transect and Random-plot methods did not differ (p ? 0.05) with respect to the number of L3 recovered from the pasture, and a positive correlation was found between them, suggesting agreement with one another, being that when the number of L3 recovered by the W-transect method increases, the same occurs in the Random-plot technique. The Random-plot method, which is already used to collect samples of forage for chemical analyses, can also be employed to estimate the pasture contamination by L3. The W-transect and Random-plot methods showed to be important in the epidemiological study of gastrointestinal nematodes in sheep. Therefore, the use of both on the same occasions and with different purposes, with one complementing the information that is not provided by the other, may be more effective in the investigation of environmental contamination by L3 of gastrointestinal nematodes.

Keywords


Epidemiology; Lambs; Methods of sampling; Nematoda.

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ALMEIDA, F. A.; PIZA, M. L. S. T.; BASSETO, C. C.; STARLING, R. Z. C.; ALBUQUERQUE, A. C. A.; PROTES, V. M.; PARIZ, C. M.; CASTILHOS, A. M.; COSTA, C.; AMARANTE, A. F. T. Infection with gastrointestinal nematodes in lambs in different integrated crop-livestock systems (ICL). Small Ruminant Research, Amsterdam, v. 166, n. 1, p. 66-72, 2018.

ALVARES, C. A.; STAPE, J. L.; SENTELHAS, P. C.; MORAES, G. de; LEONARDO, J.; SPAROVEK, G. Köppen's climate classification map for Brazil. Meteorologische Zeitschrift, Stuttgart, v. 22, n. 6, p. 711-728, 2013.

AMARANTE, A. F. T.; BARBOSA, M. A. Comparison between pasture sampling and tracer lambs to evaluate contamination of sheep pasture by nematode infective larvae. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 7, n. 2, p. 95-99, 1998.

AMARANTE, A. F. T.; ROCHA, R. A.; BRICARELLO, P. A. Relationship of intestinal histology with the resistance to Trichostrongylus colubriformis infection in three breeds of sheep. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 27, n. 1, p. 43-48, 2007.

BERNARDES, G. M. C.; CARVALHO, S.; PIRES, C. C.; MOTTA, J. H.; TEIXEIRA, W. S.; BORGES, L. I.; FLEIG, M.; PILECCO, V. M.; FARINHA, E. T.; VENTURINI, R. S. Consumo, desempenho e análise econômica da alimentação de cordeiros terminados em confinamento com o uso de dietas de alto grão. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 67, n. 6, p. 1684-1692, 2015.

BURKE, J. M.; MILLER, J. E.; TERRILL, T. H.; SMYTH, E.; ACHARYA, M. Examination of commercially available copper oxide wire particles in combination with albendazole for control of gastrointestinal nematodes in lambs. Veterinary Parasitology, Amsterdam, v. 215, n. 1, p. 1-4, 2016.

CARNEIRO, R. D.; AMARANTE, A. F. T. D. Seasonal effect of three pasture plants species on the free-living stages of Haemonchus contortus. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 60, n. 4, p. 864-872, 2008.

COHEN, J. A coefficient of agreement for nominal scales. Educational and Psychological Measurement, Thousand Oaks, v. 20, n. 1, p. 37-46, 1960.

ESCOBEDO, J. F.; GOMES, E. N.; OLIVEIRA, A. P.; SOARES, J. Ratios of UV, PAR and NIR components to global solar radiation measured at Botucatu site in Brazil. Renewable Energy, Limassol, v. 36, n. 1, p. 169-178, 2011.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS - FAO. World reference base for soil resources 2006: a framework for international classification, correlation and communication. Rome: World Soil Resources Reports No. 103, 2006. Available at: http://www.fao.org/3/a-a0510e.pdf>. Accessed at: 1 nov. 2017.

GETTINBY, G.; MCKELLAR, Q. A.; BAIRDEN, K.; THEODORIDIS, Y.; WHITELAW, A. Comparison of two techniques used for the recovery of nematode infective larvae from pasture. Research in Veterinary Science, Oxford, v. 39, n. 1, p. 99-102, 1985.

HECKLER, R. P.; BORGES, F. A. Climate variations and the environmental population of gastrointestinal nematodes of ruminants. Nematoda, Campo dos Goytacases, v. 3, n. 1, p. 1-11, 2016.

MARTIN, R. R.; BEVERIDGE, I.; PULLMAN, A. L.; BROWN, T. H. A modified technique for the estimation of the number of infective nematode larvae present on pasture, and its application in the field under South Australian conditions. Veterinary Parasitology, Amsterdam, v. 37, n. 2, p. 133-143, 1990.

MCMENIMAN, N. P. Methods of estimating intake of grazing animals. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., Juiz de Fora, 1997. Anais... Juiz de Fora: Sociedade Brasileira de Zootecnia, 1997. p. 131-168.

MORAES, E. H. B. K.; PAULINO, M. F.; ZERVOUDAKIS, J. T.; VALADARES FILHO, S. C.; MORAES, K. A. K. Avaliação qualitativa da pastagem diferida de Brachiaria decumbens Stapf.; sob pastejo, no período da seca, por intermédio de três métodos de amostragem. Revista Brasileira de Zootecnia, Viçosa, MG, v. 34, n. 1, p. 30-35, 2005.

NIEZEN, J. H.; MILLER, C. M.; ROBERTSON, H. A.; WILSON, S. R.; MACKAY, A. D. Effect of topographical aspect and farm system on the population dynamics of Trichostrongylus larvae on a hill pasture. Veterinary Parasitology, Amsterdam, v. 78, n. 1, p. 37-48, 1998.

PARIZ, C. M.; COSTA, C.; CRUSCIOL, C. A. C.; CASTILHOS, A. M.; MEIRELLES, P. R. L.; ROÇA, R. O.; PINHEIRO, R. S. B.; KUWAHARA, F. A.; MARTELLO, J. M.; CAVASANO, F. A.; YASUOKA, J. I, SARTO, J. R. W.; MELO, V. F. P.; FRANZLUEBBERS, A. J. Lamb production responses to grass grazing in a companion crop system with corn silage and oversowing of yellow oat in a tropical region. Agricultural Systems, Amsterdam, v. 151, n. 1, p. 1-11, 2017a.

PARIZ, C. M.; COSTA, C.; CRUSCIOL, C. A. C.; MEIRELLES, P. R. L.; CASTILHOS, A. M.; ANDREOTTI, M.; COSTA, N. R.; MARTELLO, J. M.; SOUZA, D. M.; PROTES, V. M.; LONGHINI, V. Z.; FRANZLUEBBERS, A. J. Production, nutrient cycling and soil compaction to grazing of grass companion cropping with corn and soybean. Nutrient Cycling in Agroecosystems, New York, v. 108, n. 1, p. 35-54, 2017b.

PARIZ, C. M.; COSTA, C.; CRUSCIOL, C. A. C.; MEIRELLES, P. R. L.; CASTILHOS, A. M.; ANDREOTTI, M.; COSTA, N. R.; MARTELLO, J. M.; SOUZA, D. M.; SARTO, J. R. W.; FRANZLUEBBERS, A. J. Production and soil responses to intercropping of forage grasses with corn and soybean silage. Agronomy Journal, Madison, v. 108, n. 6, p. 2541-2553, 2016.

SANTOS, M. C.; SILVA, B. F.; AMARANTE, A. F. T. Environmental factors influencing the transmission of Haemonchus contortus. Veterinary Parasitology, Amsterdam, v. 188, n. 3, p. 277-284, 2012.

TAYLOR, E. L. Technique for the estimation of pasture infestation by strongyloid larvae. Parasitology, Cambridge, v. 31, n. 4, p. 473-478, 1939.

UENO, H.; GONÇALVES, P. C. Manual para diagnóstico das helmintoses de ruminantes. 4th ed. Tóquio: Japan International Cooperation Agency, 1998. 145 p.

VAN DIJK, J.; LOUW, M. D. E.; KALIS, L. P. A.; MORGAN, E. R. Ultraviolet light increases mortality of nematode larvae and can explain patterns of larval availability at pasture. International Journal for Parasitology, Victoria, v. 39, n. 10, p. 1151-1156, 2009.

VAN ZYL, E. A.; BOTHA, F. S.; ELOFF, K. J. N.; MSUNTSHA, P. P.; OOSTHUIZEN, P. A.; STEVENS, C. The use of Lespedeza cuneata for natural control of gastrointestinal nematodes in Merino sheep. Onderstepoort Journal of Veterinary Research, Durbanville, v. 84, n. 1, p. 1-7, 2017.

VERSCHAVE, S. H.; LEVECKE, B.; DUCHATEAU, L.; VERCRUYSSE, J.; CHARLIER, J. Measuring larval nematode contamination on cattle pastures: comparing two herbage sampling methods. Veterinary Parasitology, Amsterdam, v. 210, n. 3, p. 159-166, 2015.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n2p712

Semina: Ciênc. Agrár.
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DOI: 10.5433 / 1679-0359
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