Medulloblastoma in a calf of the flemish breed

Thierry Grima de Cristo, Maicon Gaissler Lorena Pinto, Thais Coelho Valente, Ana Karina Couto Hack, Joandes Henrique Fonteque, Natalia Coelho Couto de Azevedo Fernandes, Cinthya dos Santos Cirqueira Borges, Renata Assis Casagrande


Medulloblastomas are neuroectodermal tumors of embryonic origin developing in cerebellum and spinal cord and have an unusual occurrence. When occurs in cattle, it is observed in neonatal cases, leading to multiple neurological clinical signs. Flemish cattle are considered at risk of extinction and the rare specimens of Brazil are in Lages, Santa Catarina. The case of a two-month-old calf with difficulty to remain in a standing position, imbalance, opisthotonus, strabismus, and broad-based gait is described in this study. The animal was euthanized and the necropsy showed an irregular, whitish pink, and friable mass in the cerebellar vermis region, measuring 5×6×3.8 cm associated with the dilation of the lateral ventricles, which extended through the interventricular foramen, midbrain aqueduct, and fourth ventricle. Histologically, it presented elongated triangular neuronal cells arranged in a dense sheet that sometimes encircled small areas of neuropil to form Homer-Wright pseudorosettes. These tumor characteristics were compatible with a medulloblastoma. The immunohistochemical (IHC) evaluation of the tumor demonstrated a positive staining for vimentin in neoplastic cells and glial fibrillary acidic protein in neoplastic stromal cells, non-reactive for synaptophysin, and negative for S100 protein and pan-cytokeratin. The histological and topographical characteristics were paramount for determining the medulloblastoma diagnosis and the IHC panel is similar to that observed in other studies. Tumor growth is limited by skull bony structures, allowing determining that the clinical signs expressed by the animal were directly related to the compression of important functional structures due to tumor expansion. Medulloblastoma is an unusual tumor in all animal species, not previously reported in Flemish cattle, and necropsy followed by histopathological examination is essential for diagnosis.


Neoplasm; Neuropathology; Central nervous system; Ruminant.

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AGERHOLM, J. S.; HEWICKER-TRAUTWEIN, M.; PEPERKAMP, K.; WINDSOR, P. A. Virus-induced congenital malformations in cattle. Acta Veterinaria Scandinavica, London, v. 57, n. 1, p. 1-14, 2015. DOI: 10.1186/s13028-015-0145-8

BIANCHI, E.; BOMBARDI, C.; BASSI, P.; BOLCATO, M.; GENTILE, A.; MILITERNO, G. Bilateral trochlear nerve palsy as a consequence of cerebellar medulloblastoma: Clinical and pathological findings in a calf. Journal of Veterinary Internal Medicine, Philadelphia, v. 29, n. 4, p. 1117-1121, 2015. DOI: 10.1111/jvim.13568

FANKHAUSER, R.; FATZER, R.; HERMANN, M. Medulloblastome bei verschiedengeschlechtigen Zwillingskälbern. Schweizer Archiv fur Tierheilkunde, Zürich, v. 124, n. 7, p. 363-367, 1982. DOI: 10.5169/seals-588251

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS - FAO. World watch list for domestic animal diversity. 3th ed. Rome: FAO, 2000. 726 p.

GOETTEN, A. L. F. Flamenga, uma raça em extinção: caracterização do desenvolvimento folicular de bovinos da raça flamenga no sul do Brasil. 2013. Dissertação (Mestrado em Ciência Animal) - Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Lages.

HIGGINS, R. J.; BOLLEN, A. W.; DICKINSON, P. J. Nervous system. In: MEUTEN, D. J. (Ed.). Tumors in domestic animals. 5th ed. Iowa: John Wiley & Sons, 2017. p. 834-891.

HILBE, M.; PETERHANS, E.; HAESSIG, M.; NUSSBAUMER, M.; EGLI, C.; SCHELP, C.; ZLINSZKY, K.; EHRENSPERGER, F. Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves. Journal of Veterinary Diagnostic Investigation, Columbia, v. 19, n. 1, p. 28-34, 2007. DOI: 10.1177/104063870701900105

MEURER, R. T.; MARTINS, D. T.; HILBIG, A.; RIBEIRO, M. C.; ROEHE, A. V.; BARBOSA-COUTINHO, L. M.; FERNANDES, M. C. Immunohistochemical expression of markers KI-67, NeuN, synaptophysin, p53 and HER2 in medulloblastoma and its correlation with clinicopathological parameters. Arquivos de Neuro-Psiquiatria, São Paulo, v. 66, n. 2 B, p. 385-390, 2008. DOI: 10.1590/S0004-282X2008000300020

SALIKI, J. T.; DUBOVI, E. J. Laboratory diagnosis of bovine viral diarrhea virus infections. Veterinary Clinics of North America - Food Animal Practice, Philadelphia, v. 20, n. 1, p. 69-83, 2004. DOI: 10.1016/j.cvfa.2003.11.005

SILVA, R. J.; AFONSO, J. A. B.; MENDONÇA, C. L.; DANTAS, A. C.; GUIMARÃES, J. A.; DANTAS, A. F.; TORRES, M. B. A. M.; KOMMERS, G. D. Medulloblastoma in a calf: case report. Brazilian Journal of Veterinary Pathology, São Paulo, v. 8, n. 1, p. 29-32, 2015.

SMITH, M. O.; GEORGE, L. W. Diseases of the nervous system. In: SMITH, B. P. (Ed.). Large animal internal medicine. 4th ed. Saint Louis: Mosby, 2009. p. 972-1111.

STEINBERG, H.; PEEK, S. F.; NELSON, K. M. Neuroblastoma with neuronal differentiation in the spinal cord in an Aberdeen Angus heifer calf. Veterinary Pathology, Thousand Oaks, v. 43, n. 2, p. 193-7, 2006. DOI: 10.1354/vp.43-2-193

WEBER, M. N.; SILVEIRA, S.; MACHADO, G.; MÓSENA, A. C.; BUDASZEWSKI, R. F.; DUPONT, P. M.; COBELLINI, L. G.; CANAL, C. W. High frequency of bovine viral diarrhea virus type 2 in Southern Brazil. Virus Research, Amsterdam, v. 191, p. 117-24, 2014. DOI: 10.1016/j.virusres.2014.07.035


Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional