Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil

Maerle Oliveira Maia, André Luís Santos de Freitas, Jamila Guimarães Santos, Thábata dos Anjos Pacheco, Dirceu Guilherme de Souza Ramos, Glaucenyra Cecília Pinheiro da Silva, Valéria Dutra, Daniel Moura Aguiar, Richard Campos Pacheco

Abstract


In Brazil, the most important tickborne pathogens affecting dogs include Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis. Babesia spp. and Hepatozoon spp., transmitted by ixodid ticks, have been reported to naturally infect dogs and are widespread. The authors aimed to investigate the incidence of B. vogeli and Hepatozoon spp. infection using molecular methods to identify factors associated with the infection in dogs from urban areas of Cuiabá municipality, Midwestern Brazil. Polymerase chain reaction (PCR) assay revealed a prevalence of 9.36% (Confidence Interval-CI 95%; 2.72%; 6.79%) and 9.61% (CI 95%; 7.0%; 13.0%) among dogs for B. vogeli and Hepatozoon, respectively. DNA sequences obtained from 10 Hepatozoon PCR positive samples were sequenced and were identical to one another and, moreover, were 100% (541/541 base of pairs-bp) homologous to the corresponding 18S rDNA sequences of H. canis. Twenty-five dogs (6.02%) generated amplicons using PCR protocols for both organisms, indicating co-infection by these two protozoans. To the best of our knowledge, our study was the first molecular survey to consider the entire population of dogs from the study area. Moreover, young dogs (0-12 months of age), as well as animals living in walled houses?without access to the street?were more susceptible to infection with B. vogeli and H. canis, respectively.

Keywords


Canine babesiosis; Canine hepatozoonosis; PCR; Tick-borne diseases.

Full Text:

PDF

References


ALMEIDA, A. P.; MARCILI, A.; LEITE, R. C.; NIERI-BASTOS, F. A.; DOMINGUES, L. N.; MARTINS, J. R.; LABRUNA, M. B. Coxiella symbiont in the tick Ornithodoros rostratus (Acari: Argasidae). Ticks and Tick-borne Diseases, Amsterdam, v. 3, n. 4, p. 203-206, 2012. DOI: 0.1016/j.ttbdis.2012.02.003

BIRKENHEUER, A. J.; LEVY, M. G.; BREITSCHWERDT, E. B. Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. Journal of Clinical Microbiology, Washington, v. 41, n. 9, p. 4172-4177, 2003. DOI: 10.1128/JCM.41.9.4172-4177.2003

DANTAS-TORRES, F.; FIGUEREDO, L. A. Canine babesiosis: a Brazilian perspective. Veterinary Parasitology, Amsterdam, v. 141, n. 3-4, p. 197-203, 2006. DOI: 10.1016/j.vetpar.2006.07.030

DEMONER, L. C.; MAGRO, N. M.; SILVA, M. R. L. da; PAULA ANTUNES, J. M. A. de; CALABUIG, C. I. P.; O'DWYER, L. H. Hepatozoon spp. infections in wild rodents in an area of endemic canine hepatozoonosis in southeastern Brazil. Ticks and Tick-borne Diseases, Amsterdam, v. 7, n. 5, p. 859-864, 2016. DOI: 10.1016/j.ttbdis.2016.04.002

DEMONER, L. C.; RUBINI, A. S.; PADUAN, K. S.; METZGER, B.; PAULA ANTUNES, J. M. de; MARTINS, T. F.; MATHIAS, M. I.; O'DWYER, L. H. Investigation of tick vectors of Hepatozoon canis in Brazil. Ticks and Tick-borne Diseases, Amsterdam, v. 4, n. 6, p. 542-546, 2013. DOI: 10.1016/j.ttbdis.2013.07.006

DUARTE, S. C.; LINHARES, G. F. C.; ROMANOWSKY, T. N.; SILVEIRA, O. J. N. O.; BORGES, L. M. F. Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay. Veterinary Parasitology, Amsterdam, v. 152, n. 1-2, p. 16-20, 2008. DOI: 10.1016/j.vetpar.2007.12.013

GOMES, L. A.; MORAES, L. A.; AGUIAR, D. C. F.; DIAS, H. L. T.; RIBEIRO, A. S. S.; ROCHA, H. P. C.; NUNES, M. R. T.; GONÇALVES, E.C. Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil. Ticks and Tick-borne Diseases, Amsterdam, v. 7, n. 6, p. 1061-1066, 2016. DOI: 10.1016/j.ttbdis.2016.09.008

IRWIN, P. J.; HUTCHINSON, G. W. Clinical and pathological findings of Babesia infection in dogs. Australian Veterinary Journal, Victoria, v. 68, n. 6, p. 204-209, 1991. DOI: 10.1111/j.1751-0813.1991.tb03194.x

JOHNSON, E. M.; ALLEN, K. E.; PANCIERA, R. J.; EWING, S. A.; LITTLE, S. E. Experimental transmission of Hepatozoon americanum to New Zealand white rabbits (Oryctolagus cuniculus) and infectivity of cystozoites for a dog. Veterinary Parasitology, Amsterdam, v. 164, n. 2-4, p. 162-166, 2009. DOI: 10.1016/j.vetpar.2009.05.028

LABRUNA, M. B.; HOMEM, V. S. F.; HEINEMANN, M. B.; FERREIRA NETO, J. S. Ticks (Acari: Ixodidae) associated with rural dogs in Uruará, eastern Amazon, Brazil. Journal of Medical Entomology, Annapolis, v. 37, n. 5, p. 774-776, 2000. DOI: 10.1603/0022-2585-37.5.774

MIRANDA, R. L.; O’DWYER, L. H.; CASTRO, J. R. de; METZGER, B.; RUBINI, A. S.; MUNDIM, A. V.; EYAL, O.; TALMI-FRANK, D.; CURY, M. C.; BANETH, G. Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil. Research in Veterinary Science, London, v. 97, n. 2, p. 326-329, 2014. DOI: 10.1016/j.rvsc.2014.06.015

MORAES, P. H. G.; RUFINO, C. P.; BARAÚNA, A. R. F.; REIS, T.; AGNOL, L. T. D.; MENESES, A. M. C.; AGUIAR, D. C. F.; NUNES, M. R. T.; GONÇALVES, E. C. Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil. Genetics and Molecular Research, Ribeirão Preto, v. 14, n. 4, 16364-16371, 2015. DOI: Vertical transmission of Hepatozoon canis in dog

MURATA, T.; INOUE, M.; TATEYAMA, S.; TAURA, Y.; NAKAMA, S. Vertical transmission of Hepatozoon canis in dogs. Journal of Veterinary Medical Science, Tokyo, v. 55, n. 5, p. 867-868, 1993. DOI: 10.1292/jvms.55.867

O’DWYER, L. H. Brazilian canine hepatozoonosis. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 3, p. 181-193, 2011. DOI: 10.1590/S1984-29612011000300002

ROTONDANO, T. E. F.; ALMEIDA, H. K. A.; KRAWCZAK, F. S.; SANTANA, V. L.; VIDAL, I. F.; LABRUNA, M. B.; AZEVEDO, S. S.; ALMEIDA, A. M. P.; MELO, M. A. Survey of Ehrlichia canis, Babesia spp. and Hepatozoon spp. in dogs from a semiarid region of Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 24, n. 1, p. 52-8.31, 2015. DOI: 10.1590/S1984-29612015011

RUBINI, A. S.; PADUAN, K. S.; MARTINS, T. F.; LABRUNA, M. B.; O’DWYER, L. H. Acquisition and transmission of Hepatozoon canis (Apicomplexa: Hepatozoidae) by the tick Amblyomma ovale (Acari: Ixodidae). Veterinary Parasitology, Amsterdam, v. 164, n. 2-4, p. 324-327, 2009. DOI: 10.1016/j.vetpar.2009.05.009

SAMBROOK, J.; RUSSELL, D. W. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory Press, 2001. 2100 p.

SINGLA, L.; SUMBRIA, D.; MANDHOTRA, A.; BAL, M. S.; KAUR, P. Critical analysis of vector-borne infections in dogs: Babesia vogeli, Babesia gibsoni, Ehrlichia canis and Hepatozoon canis in Punjab, India. Acta Parasitologica, Warsaw, v. 61, n. 4, p. 697-706, 2016. DOI: 10.1515/ap-2016-0098

SPOLIDORIO, M. G.; TORRES, M. M.; CAMPOS, W. N.; MELO, A. L.; IGARASHI, M.; AMUDE, A. M.; LABRUNA, M. B.; AGUIAR, D. M. Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 3, p. 253-55, 2011. DOI: 10.1590/S1984-29612011000300015

SOUSA, K. C. de; FERNANDES, M. P.; HERRERA, H. M.; BENEVENUTE, J. L.; SANTOS, F. M.; ROCHA, F. L.; BARRETO, W. T.; MACEDO, G. C.; CAMPOS, J. B.; MARTINS, T. F.; de ANDRADE PINTO, P. C.; BATTESTI, D. B.; PIRANDA, E. M.; CANÇADO, P. H.; MACHADO, R. Z.; ANDRÉ, M. R. Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route. Veterinary Parasitology, Amsterdam, v. 15, n. 237, p. 37-46, 2017. DOI: 10.1016/j.vetpar.2017.02.023

VINCENT-JOHNSON, N. Canine and feline Hepatozoonosis. In: SYKES, J. Canine and feline infectious diseases. Saint Louis: Elsevier, 2014. cap. 77, p. 747-759.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n3p1357

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional