Diet and breed alter community structures of rumen protozoa in cattle subjected to different feeding systems

Cândida Camila dos Reis, Emilyn Midori Maeda, Franciane Cedrola, Elias Nunes Martins, Fabiana Matielo De Paula, Isabel Martinele

Abstract


The objective were to identify and quantify the rumen protozoa from two genetic groups of beef cattle (Nellore and crossbred European beef cattle) subjected to three feeding systems (pasture, pasture supplement, and confined). Samples of rumen contents were obtained from the center of the ruminal mass after slaughter. Quantification and identification of rumen protozoa were performed with a Sedgewick-Rafter counting chamber by optic microscopy. Data were analyzed using general linear analysis methodology with a Poisson distribution (1 %). In addition, the data underwent of Analisys Principal Component. A effect on ruminal protozoa was observed for the diets and breeds analyzed. Was identified 14 genera, among which Entodinium was predominant in all animals analyzed. Ciliates belonging to Entodiniomorphida, Eodinium, Epidinium, Eremoplastron, Eudiplodinium, Metadinium, and Ostracodinium showed highest density in Nellore animals when compared with the crossbreed, Nellore × European. Larger numbers of protozoa from the family Isotrichidae (Dasytricha and Isotricha) were observed in animals maintained by pasture or pasture supplement feeding. According to the kind of feeding, those fed exclusively with pasture had a greater diversity of ciliates in comparison to those restricted to, or supplemented with pasture. In one animal, the occurrence of ciliates belonging to the genus Buetschlia, was observed (prevalence of 1.66 %), which is one of only a few records of this genus in ruminants.

Keywords


Breed; Cattle; Isotrichidae; Ophryoscolecidae; Pasture.

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References


ALIPOUR, D. Ruminal ciliated protozoa in baloochi and sindhi camel breeds. Journal of Veterinary Research, Napoli, v. 67, n. 3, p. 257-263, 2012.

ARTMANN, A. T.; TOMA, S. H.; PINHEIRO, N. J.; ROMERO, J.; CARVALHO, A. M.; MONTEIRO, T. Breeding and cattle ½ blood taurino x ½ blood zebu in Brazil. Revista Científica de Medicina Veterinária, Garça, ano XII, n. 22, p. 1-10, 2014.

BONHOMME-FLORENTIN, A. Contribution à l’lètude de La physiologie dês ciliés entodiniomorphes endocommensaux des ruminats et des équidés. Annales des Sciences Naturelles, London, v. 16, n. 12, p. 155-283, 1974.

CEDROLA, F.; MARTINELE, I.; DIAS, R. J. P.; FREGULIA, P.; D’AGOSTO, M. Rumen ciliates in Brazilian sheep (Ovis aries) and redescription of Entodinium contractum (Entodiniomorphida, Ophryoscolecidae). Zootaxa, New Zealand, v. 4088, n. 2, p. 292-300, 2016.

CERSOSIMO, L. M.; BAINBRIDGE, M. L.; WRIGHT, A. D. G.; KRAFT, J. Breed and lactation stage alter the rumen protozoal fatty acid profiles and community structures in primiparous dairy cattle. Journal of Agricultural and Food Chemistry, Washington, v. 64, n. 9, p. 2021-2029, 2016.

D’AGOSTO, M.; CARNEIRO, M. E. Evaluation of lugol solution used for counting rúmen ciliates. Revista Brasileira de Zoologia, Curitiba, v. 16, n. 3, p. 725-729, 1999.

DEHORITY, B. A. Evaluation of subsampling and fixation procedures used for counting rumen protozoa. Applied and Environmental Microbiology, Washington, v. 48, n. 1, p. 182-185, 1984.

DEHORITY, B. A. Protozoa of the digestive tract of herbivorous mammals. Insect Science and its Applications, Cambridge, v. 7, n. 3, p. 279-296, 1986.

DEHORITY, B. A. Rumen microbiology. Nottinghan: Nottinghan University Press, 2003. 380 p.

GEBIOMET - GRUPO DE ESTUDOS EM BIOMETEOROLOGIA. Dois Vizinhos: Universidade Tecnológica Federal do Paraná, 2014. Disponível em: www.gebiomet.com.br. Acesso em: 20 jan. 2015.

HUWS, S. A.; KIM, E. J.; KINGSTON-SMITH, A. H.; LEE, M. R. F.; MUETZEL, S. M.; COOKSON, A. R.; NEWBOLD, C. J.; WALLACE, R. J.; SCOLLAN, N. D. Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts. FEMS Microbiology Ecology, Bethesda, v. 69, n. 3, p. 461-471, 2009.

MANELLA, M. Q.; LOURENÇO, A. J. População de protozoários ciliados no rúmen de bovinos nelore em pastos de Brachiaria brizantha Marandu recebendo suplemento protéico ou com livre acesso a banco de proteína de Leucaena leucocephala nas diferentes estações do ano. Boletim de Indústria Animal, Nova Odessa, v. 61, n. 1, p. 1-11, 2004.

MARTINELE, I.; SANTOS, G. R. A.; MATOS, D. S.; BATISTA, A. M. V.; D’AGOSTO, M. Diet botanical composition and rumen protozoa of sheep in brazilian semi-arid. Archivos de Zootecnia, Cordoba, v. 59, n. 226, p. 169-175, 2010.

MARTINELE, I.; SANTOS, G. R. A.; MATOS, D. S.; BATISTA, A. M. V.; D’AGOSTO, M. Protozoários ciliados no rúmen de ovinos mestiços mantidos em pastagem natural de caatinga. Revista Brasileira de Saúde e Produção Animal, Salvador, v. 9, n. 2, p. 280-292, 2008.

NEWBOLD, C. J.; de la FUENTE, G.; BELANCHE, A.; RAMOS-MORALES, E.; MCEWAN, N. R. The role of ciliate protozoa in the rumen. Frontiers in Microbiology, Lausanne, v. 1313, n. 6, p. 1-7, 2015.

OGIMOTO, K.; IMAI, S. Atlas of rumen microbiology. Tokyo: Japan Scientificv Societies, 1981. 268 p.

O'KELLY, J. C.; SPIERS, W. G. Possible contribution of protozoa to diferences in rumen metabolism between cattle breeds. Australian Journal of Agricultural Research, Mailing, v. 43, n. 8, p. 1795-1808, 1992.

SILVA, D. J.; QUEIROZ, A. C. Análises de alimentos: métodos químicos e biológicos. 3. ed. Viçosa, MG: Editora UFV, 2004, 235 p.

SISTEMA PARA ANÁLISES ESTATÍSTICAS - SAEG-. Versão 9.1: Fundação Arthur Bernardes – UFV - Viçosa, MG, 2007.

STATISTICAL ANALYSIS SYSTEM - SAS. System for Microsoft Windows: release 8.2. New York: Cary, 2001.

VOIGT, J.; JENTSCH, W.; KUHLA, S.; MATTHES, H. D.; DERNO, M. Rumen fermentation and retention time of the digesta in growing cattle of the breeds Black-White Dairy Cattle, Galloway, and Highland. Arch Tierz, Dummerstorf, v. 43, n. 6, p. 606-620, 2000.

WILLIAMS, A. G.; COLEMAN, G. S. The rumen protozoa. New York: Springer-Verlag, 1992, 423 p.

WRIGHT, A. D. Rumen protozoa. In: PUNIYA, A. K.; SINGH, R.; KAMRA, D. N. Rumen microbiology: from evolution to revolution. New York: Springer-Verlag, 2015, p. 113-120.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n2p909

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional