Addition of different probiotics to commercial feeds for piglets during the nursery phase

Newton Tavares Escocard de Oliveira, Paulo Levi de Oliveira Carvalho, Jansller Luiz Genova, Eliseu Carlos Cristofori, Fábio Henrique Rodrigues Silveira, Osiris Antunes de Caxias Junior, Ana Lúcia Almeida Santana

Abstract


In this study, we aimed to evaluate the effect of the addition of different probiotics to commercial diets for piglets on diet digestibility, total bacterial count (TBC) of faeces, Clostridium count and growth performance. In the digestibility assay, 24 entire male pigs with an average initial body weight of 18.8 ± 0.87 kg were housed individually in metabolism cages and distributed in a completely randomised experimental design; this consisted of three treatments (probiotic-free commercial feed; commercial feed with the addition of 0.2% probiotic containing a blend of microorganisms; commercial feed with the addition of 0.1% yeast-based probiotic Saccharomyces cerevisiae boulardii) and eight replicates. The digestibility of the feeds was determined. At the end of the experiment, 25 grams of faeces were collected for microbiological analysis. In the subsequent experiment, 150 crossbred piglets (entire males and females), weaned at 21 days of age and having an average initial body weight of 6.81 ± 0.71 kg were distributed in a randomised complete block design, with six treatments repeated twice in the first and thrice in the second block, totaling five replicates and five pigs per experimental unit. The treatments, arranged in a 3 x 2 factorial arrangement, consisted of a combination of the three experimental diets used in the digestibility test and two genders. The growth performance in the nursery was evaluated during the following phases: pre-starter I (21 to 29 days), pre-starter I and II (21 to 40 days) and the total period (21 to 63 days). There was an effect (p < 0.05) of the diets on the digestible protein and the logarithm of the TBC. There was no effect (p > 0.05) of the interaction or the diets on the growth performance of the pigs in any of the phases. Both in the entire experimental period and in the pre-starter I and II phases, male pigs presented better feed conversion (p < 0.05) than females. There was an improvement in the apparent digestibility of the crude protein of the feed when the pigs were fed commercial feed containing probiotics. Pigs fed commercial feed containing probiotics based on yeast Saccharomyces cerevisiae boulardii presented a reduced TBC in their faeces. Commercial feed containing probiotics did not influence the growth performance parameters of nursery phase piglets.

Keywords


Additives; Growth performance; Lactobacillus; Microbiology; Saccharomyces; Yeast.

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References


AFONSO, E. R.; PARISSA, L. J.; MARINO, C. T.; MARTINS, S. M. K.; SILVA, C. C.; GAMEIRO, A. H.; MORETTI, A. S. A. Associação de probióticos adicionados à dieta de leitões no aleitamento e na creche: índices zootécnicos e economicidade. Revista Brasileira de Saúde Produção Animal, Salvador, v. 14, n. 1, p. 161-176, 2013.

AHMED, S. T.; HOON, J.; MUN, H. S.; YANG, C. J. Evaluation of Lactobacillus and Bacillus-based probiotics as alternatives to antibiotics in enteric microbial challenged weaned piglets. African Journal of Microbiology Research, Nigeria, v. 8, n. 1, p. 96-104, 2014.

BREVES, G.; WALTER, C.; BURMESTER, M.; SCHRODER, B. In vitro studies on the effects of Saccharomyces boulardii and Bacillus cereus var. toyoi on nutrient transport in pig jejunum. Journal of Animal Physiology and Animal Nutrition, Berlin, v. 84, n. 1-2, p. 9-20, 2000. DOI: 10.1046/j.1439-0396.2000.00277.x

BRITO, M. B.; PLAZA-DÍAZ, J.; FONTANA, L.; MUÑOZ-QUEZADA, S. In vitro cell and tissue models for studying host-microbe interactions: a review. British Journal of Nutrition, London, v. 109, n. 2, p. 27-34, 2013. DOI: 10.1017/S0007114512004023

BRUMANO, G.; GATTÁS, G. Fatores que influenciam na exigência de lisina para suínos. Revista Eletrônica Nutritime, Viçosa, MG, v. 6, n. 3, p. 918-940, 2009.

GIANG, H. H.; VIET, T. Q.; OGLE, B.; LINDBERG, J. E. Growth performance, digestibility, gut environment and health status in weaned piglets fed a diet supplemented with a complex of acid bacteria alone or in combination with Bacillus subtilis and Saccharomyces boulardii. Livestock Science, Amsterdam, v. 143, n. 2, p. 132-141, 2012. DOI: 10.1016/j.livsci.2010.01.010

GUIMARÃES, G. G.; MURATA, L. S.; MCMANUS, C.; SANTANA, A. P.; RECKZIEGEL, G. C.; AMÂNCIO, A. S.; JARDIM FILHO, R. M.; SOBRINHO, A. J. F. Desempenho de suínos de dois cruzamentos de linhagens comerciais criados em cama sobreposta. Archivos de Zootecnia, Córdoba, v. 60, n. 229, p. 11-18, 2011. DOI: 10.4321/S0004-05922011000100002

JUNQUEIRA, O. M.; BARBOSA, L. C. G. S.; PEREIRA, A.; ARAÚJO, L. F.; GARCIA NETO, M.; PINTO, M. F. Uso de aditivos em rações para suínos nas fases de creche, crescimento e terminação. Revista Brasileira de Zootecnia, Viçosa, MG, v. 38, n. 12, p. 2394-2400, 2009. DOI: 10.1590/S1516-35982009001200015

KOTZAMPASSI, K.; GIAMARELLOS, E. J. Probiotics for infectious diseases: more drugs, less dietary supplementation. International Journal of Antimicrobial Agents, Holanda, v. 40, n. 4, p. 288-96, 2012. DOI: 10.1016/j.ijantimicag.2012.06.006

LOJANICA, M.; MANOJLOVIC, M.; JEREMIC, D. The effects of probiotic Enterococcus faecium DSM 7134 in the weaned pigs nutrition. Biotechnology in Animal Husbandry, Zemun, v. 26, n. 1-2, p. 57-64, 2010.

MATTERSON, L. D.; POTTER, L. M.; STUTZ, M. W.; SINGSEN, E. P. Research report 7: the metabolizable energy of feed ingredients for chickens. Hartford: Res. Rep. Conn. Agric. Exp. Stn., 1965. 11p.

MENG, Q. W.; YAN, L.; AO, X.; ZHOU, T. X.; WANG, J. P.; LEE, J. H.; KIM, I. H. Influence of probiotics in different energy and nutrient density diets on growth performance, nutrient digestibility, meat quality, and blood characteristics in growing-finishing pigs. Journal of Animal Science, Champaign, v. 88, n. 10, p. 3320-3326, 2010. DOI: 10.2527/jas.2009-2308

PAPATSIROS, V. G.; TASSIS, P. D.; TZIKA, E. D.; PAPAIOANNOU, D. S.; PETRIDOU, E.; ALEXOPOULOS, C.; KYRIAKIS, S. C. Effect of benzoic acid and combination of benzoic acid with a probiotic containing Bacillus Cereus var. toyoi in weaned pig nutrition. Polish Journal of Veterinary Sciences, Olsztyn-Kortowo, v. 14, n. 1, p. 117-125, 2011. DOI: 10.2478/v10181-011-0017-8

PEKAS, J. C. Versatible swine laboratory apparatus for physiologic and metabolic studies. Journal of Animal Science, Champaign, v. 27, n. 5, p. 1303-1309, 1968. DOI: 10.2527/jas1968.2751303x

POWER, S. E.; OTOOLE, P. W.; STANTON, C.; ROSS, R. P.; FITZGERALD, G. F. Intestinal microbiota, diet and health. British Journal of Nutrition, London, v. 111, n. 3, p. 387-402, 2014. DOI: 10.1017/S0007114513002560

RAO, J. N.; WANG, J. Y. Regulation of gastrointestinal mucosal growth. In: GRANGER, D. N.; GRANGER, J. P. (Ed.). Colloquium series on integrated systems physiology: from molecule to function. 2th ed. Califórnia: Morgan & Claypool Life Sciences, 2010. p. 1-114.

ROBLES-HUAYNATE, R. A.; THOMAZ, M. C.; SANTANA, A. E.; MASSON, G. C. I. H.; AMORIN, A. B.; SILVA, S. Z.; RUIZ, U. S.; WATANABE, P. H.; BUDIÑO, E. L. Probiótico em dietas de suínos sobre os parâmetros sanguíneos e digestibilidade de rações. Semina: Ciências Agrárias, Londrina, v. 35, n. 3, p. 1627-1636, 2014. DOI: 10.5433/1679-0359.2014v35n3p1627

ROSTAGNO, H. S.; ALBINO, L. F. T.; DONZELE, J. L.; GOMES, P. C.; OLIVEIRA, R. F.; LOPES, D. C.; FERREIRA, A. S.; BARRETO, S. L. T.; EUCLIDES, R. F. Tabelas brasileiras para aves e suínos: composição de alimentos e exigências nutricionais. 3. ed. Viçosa, MG: UFV, 2011. 252 p.

SAKOMURA, N. K.; ROSTAGNO, H. S. Métodos de pesquisa em nutrição de monogástricos. 2. ed. Jaboticabal: FUNEP, 2016. 283 p.

SHIM, Y. H.; SHINDE, P. L.; CHOI, J. Y.; KIM, J. S.; SEO, D. K.; PAK, J. I.; CHAE, J.; KWON, I. K. Evaluation of multi-microbial probiotics produced by submerged liquid and solid substrate fermentation methods in broilers. Asian-Aust. Journal of Animal Science, Champaign, v. 23, n. 4, p. 521-529, 2010. DOI: 10.5713/ajas.2010.90446

SILVA, N.; JUNQUEIRA, V. C. A.; SILVEIRA, N. F. A. Manual de métodos de análise microbiológica de alimentos. São Paulo: Varala, 1997. 295 p.

SILVA, D. J.; QUEIROZ, A. C. Análise de alimentos: métodos químicos e biológicos. 3th ed. Viçosa, MG: Universidade Federal de Viçosa, 2002. 235 p.

WANG, Y.; CHO, J. H.; CHEN, Y. J.; YOO, J. S.; HUANG, Y.; KIM, H. J.; KIM, I. H. The effect of probiotic BioPlus 2B® on growth performance, dry matter and nitrogen digestibility and slurry noxious gas emission in growing pigs. Livestook Science, Amsterdam, v. 120, n. 1, p. 35-42, 2009. DOI: 10.1016/j.livsci.2008.04.018

YANG, F.; HOU, C.; ZENG, X.; QIAO, S. The use of lactic acid bacteria as a probiotic in swine diets. Pathogens, Basel, v. 4, n. 1, p. 34-45, 2015. DOI: 10.3390/pathogens4010034




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n3p1225

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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