Seroconversion attributable to vaccination of heifers with the strain B-19 of Brucella abortus

Alessandro José Ferreira dos Santos, Katyane de Sousa Almeida, Francisco Baptista, Marco Augusto Giannoccaro da Silva, Bruna Alexandrino, Jardel Martins Ferreira

Abstract


Bovine brucellosis is transmitted by Brucella abortus and causes considerable economic losses to agribusiness. To determine the seroconversion rate attributable to vaccination of heifers with B. abortus strain B-19, 330 blood serum samples from 110 heifers aged three to eight months from five properties in the municipality of Araguaína, Tocantins, Brazil, were analyzed. On day zero (D-0), blood samples were collected, and 55 heifers from the intervention group were vaccinated. The remaining blood samples were collected on days seven (D-7), 14 (D-14), and 21 (D-21). The serum samples were analyzed using the buffered acidified plate antigen (BAPA) test. The rate of seroconversion attributable to vaccination was calculated by subtracting from the seroconversion incidence rate in the intervention group the incidence rate of the control group. On D-0, 100% (110/110) of the heifers were negative in the BAPA test (95% CI [96.70 - 100%], p < 0.05). On D-7, the seroconversion rate was 94.55% (52/55) (95% CI [84.88 - 98.84%], p < 0.05) in the intervention group and 0% (0/55) (95% CI [0.00 - 6.49%], p < 0.05) in the control group. On D-14, the rate was 98.18% (54/55) (95% CI [90.28 - 99.95%], p < 0.05) in the intervention group and 0% in the control group. Therefore, seroconversion attributable to vaccination varied from 78.39% (84.88 - 6.49%) to 92.35% (98.86 - 6.49%) on D-7 and from 83.79% (90.28 - 6.49%) to 93.46% (99.95 - 6.49%) on D-14, respectively. On D-21, the seroconversion rate in the 55 heifers from the control group vaccinated on D-14 was 100% (55/55) (95% CI [93.51 - 100%], p < 0.05) but was not significantly different (p > 0.05) from that of heifers from the intervention group vaccinated on D-7. The results of this study allow making recommendations to the Official Veterinary Service on establishing a vaccination audit, with random collection of blood samples starting on day seven after vaccination in heifers considered vaccinated with B. abortus strain B-19 using the BAPA test as a diagnostic test and considering a minimum seroconversion rate of 75% to evaluate vaccination against brucellosis.

Keywords


Brucellosis; Diagnosis; Monitoring; PNCEBT; Vaccination.

Full Text:

PDF

References


ALVES, A. J. S.; VILLAR, K. S. Brucelose bovina e sua situação sanitária no Brasil. Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV/SP, São Paulo, v. 9, n. 2, p. 12-17, 2011.

BAPTISTA, F.; LEITE, R. C.; HADDAD, J. P. A.; ALMEIDA, K. S.; NARDI, C. P. P. Prevalence and risk factors for brucellosis in Tocantins and brazilian national programs to fight this disease. Revista Patologia Tropical, Goiânia, v. 41, n. 3, p. 285-294, 2012. DOI: 10.5216/rpt.v41i3.20755

BRASIL. Instrução Normativa nº 10, de 03 de março de 2017. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Poder Executivo. Diário Oficial [da] União, Brasília, 2017. Edição 116, Seção 1, p. 4-8.

BRASIL. Manual de legislação: programas nacionais de saúde animal do Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Brasília: Secretaria de Defesa Agropecuária. Departamento de Saúde Animal, 2009. 440 p.

BRASIL. Manual técnico: Programa nacional de controle e erradicação da brucelose e tuberculose. Ministério da Agricultura, Pecuária e Abastecimento. Brasília: Secretaria de Defesa Agropecuária. Departamento de Saúde Animal, 2006. 188 p.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS - FAO. Guidelines for coordinated human and animal brucellosis surveillance. Rome: Animal Production and Health Division, FAO Agriculture Department, 2003. n. 156, 46 p.

FERREIRA NETO, J. S.; SILVEIRA, G. B.; ROSA, B. M.; GONÇALVES, V. S. P.; GRISI-FILHO, J. H. H.; AMAKU, M.; DIAS, R. A.; FERREIRA, F.; HEINEMANN, M. B.; TELLES, E. O.; LAGE, A. P. Analysis of 15 years of the national program for the control and eradication of animal brucellosis and tuberculosis, Brazil. Semina: Ciências Agrárias, Londrina, v. 37, n. 5, p. 3385-3402, 2016. Supplement 2. DOI: 10.5433/1679-0359.2016v37n5Supl2p3385

PAULIN, L. M.; FERREIRA NETO, J. S. O combate à brucelose bovina: situação brasileira. Jaboticabal: Fundação de Estudos e Pesquisas em Agronomia, Medicina Veterinária e Zootecnia, 2003. 154 p.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n3p1145

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional