Milk fatty acid composition of dairy goats fed increasing levels of Flemingia macrophylla hay

Isabel das Neves Oiticica, Carlos Elysio Moreira da Fonseca, Fernando César Ferraz Lopes, Mirton José Frota Morenz, Marco Antônio Sundfeld da Gama, Vinícius Carneiro de Souza, Aline Barros da Silva

Abstract


The effect of dietary inclusion of increasing levels of Flemingia macrophylla hay on the yield, composition and fatty acid profile of milk from Saanen x Boer goats was evaluated. The diets were composed of 40% concentrate and 60% forage. The treatments were defined by the level of inclusion of Flemingia hay in the diet (0, 80, 160, 240 and 320 g kg-1 dry matter) in replacement of the Cynodon dactylon cv. Tifton-85 hay, distributed according to a 5 x 5 Latin Square design. The inclusion of Flemingia in the diet did not change the milk yield or milk fat content but promoted a quadratic effect on the fat yield. A quadratic effect was observed on the milk protein and lactose contents, but this effect was not reflected in the daily production of these components. The concentrations of vaccenic, rumenic, elaidic and trans-10 C18:1 fatty acids in milk fat increased linearly in response to the dietary inclusion of Flemingia, whereas the contents of palmitic, stearic, oleic, linoleic and ?-linolenic fatty acids were not modified. A linear reduction was observed in the lauric and myristic fatty acids concentrations, which was accompanied by a reduction in the atherogenic index of milk fat. A quadratic effect was also observed on the trans-10, cis-12 CLA milk fat content in response to the inclusion of Flemingia in the diet, as well as a linear increase in the apparent transfer of ?-linolenic acid from the diet to milk. The inclusion of up to 320 g kg-1 of Flemingia hay as a replacement for Tifton-85 hay in the Saanen x Boer dairy goat diet does not modify the milk production and improves the nutritional quality of milk fat by increasing the contents of rumenic and vaccenic acids beneficial to human health and reducing the concentrations of hypercholesterolemic fatty acids.

Keywords


Conjugated linoleic acid; Fabaceae; Leguminous; Rumenic acid; Vaccenic acid.

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References


ALMEIDA, M. M.; LUQUETTI, S. C. D.; SABARENSE, C. M.; CORRÊA, J. O. A.; REIS, L. G.; CONCEIÇÃO, E. P. S.; LISBOA, P. C.; MOURA, E. G.; GAMEIRO, J.; GAMA, M. A. S.; LOPES, F. C. F.; GONZÁLEZ GARCIA, R. M. Butter naturally enriched in cis-9 trans-11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats. Lipids in Health and Disease, London, v. 13, n. 200, p. 1-13, 2014.

ALMEIDA, O. C.; PIRES, A. V.; SUSIN, I.; GENTIL, R. S.; MENDES, C. Q.; QUEIROZ, M. A. A.; FERREIRA, E. M.; EASTRIDGE, M. L. Milk fatty acids profile and arterial blood milk fat precursors concentration of dairy goats fed increasing doses of soybean oil. Small Ruminant Research, Amsterdan, v. 114, n. 1, p. 152-160, 2013.

ANDERSSON, M. S.; LASCANO, C. E.; SCHULTZE-KRAFT, R.; PETERS, M. Forage quality and tannin concentration and composition of a collection of the tropical shrub legume Flemingia macrophylla. Journal of Science and Food Agricultural, Oxford, v. 86, n. 7, p. 1023-1031, 2006.

ANDERSSON, M. S.; SCHULTZE-KRAFT, R.; PETERS, M. Flemingia macrophylla (Willd.) Merrill. Rome: FAO Grassland Index, 2002. Available at: . Accessed at: 24 aug. 2017.

BARAHONA, R.; LASCANO, C. E.; COCHRAN, R.; MORRILL, J.; TITGEMEYER, E. C. Intake, digestion, and nitrogen utilization by sheep fed tropical legumes with contrasting tannin concentration and astringency. Journal of Animal Science, Champaign, v. 75, n. 6, p. 1633-1640, 1997.

BERNARD, L.; LEROUX, C.; FAULCONNIER, Y.; DURAND, D.; SHINGFIELD, K. J.; CHILLIARD, Y. Effect of sunflower-seed oil or linseed oil on milk fatty acid secretion and lipogenic gene expression in goats fed hay-based diets. Journal of Dairy Research, Cambridge, v. 76, n. 2, p. 241-248, 2009.

BERNARD, L.; LEROUX, C.; ROUEL, J.; DELAVAUD, C.; SHINGFIELD, K. J.; CHILLIARD, Y. Effect of extruded linseeds alone or in combination with fish oil on intake, milk production, plasma metabolite concentrations and milk fatty acid composition in lactating goats. Animal, Cambridge, v. 9, n. 5, p. 810-821, 2015.

BERNARD, L.; MOURIOT, J.; ROUEL, J.; GLASSER, F.; CAPITAN, P.; PUJOS-GUILLOT, E.; CHARDIGNY, J.-M.; CHILLIARD, Y. Effects of fish oil and starch added to a diet containing sunflower-seed oil on dairy goat performance, milk fatty acid composition and in vivo ?9-desaturation of [13C]vaccenic acid. British Journal of Nutrition, Cambridge, v. 104, n. 3, p. 346-354, 2010.

BUCCIONI, A.; DECANDIA, M.; MINIERI, S.; MOLLE, G.; CABIDDU, A. Lipid metabolism in the rumen: New insights on lipolysis and biohydrogenation with an emphasis on the role of endogenous plant factors. Animal Feed Science and Technology, Amsterdan, v. 174, n. 1-2, p. 1-25, 2012.

CHILLIARD, Y.; FERLAY, A. Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reproduction Nutrition Development, Cambridge, v. 44, n. 5, p. 467-492, 2004.

CÍVICO, A.; SÁNCHEZ, N. N.; GÓMEZ-CORTÉS, P.; de la FUENTE, M. A.; BLANCO, F. P.; JUÁREZ, M.; SCHIAVONE, A.; MARTÍNEZ MARÍN, A. L. Odd- and branched-chain fatty acids in goat milk as indicators of the diet composition. Italian Journal of Animal Science, Abingdon, v. 16, n. 1, p. 68-74, 2017.

FAGUNDES, G. M. Desempenho produtivo e composição do leite de cabras alimentadas com dietas contendo diferentes níveis de Flemingia macrophylla (willd.) Merrill com e sem Polietilenoglicol. 2012. Dissertação (Mestrado em Ciências) - Universidade Federal Rural do Rio de Janeiro, Seropédica.

FAGUNDES, G. M.; MODESTO, E. C.; FONSECA, C. E. M.; LIMA, H. R. P.; MUIR, J. P. Intake, digestibility and milk yield in goats fed Flemingia macrophylla with or without polyethylene glycol. Small Ruminant Research, Amsterdan, v. 116, n. 2-3, p. 88-93, 2014.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS - FAO. Fats and fatty acids in human nutrition. Report of an expert consultation. Roma: FAO, 2010. 166 p.

JACKSON, F. S.; BARRY, T. N.; LASCANO, C.; PALMER, B. The extractable and bound condensed tannin content of leaves from tropical tree, shrub and forage legumes. Journal of the Science of Food and Agriculture, Oxford, v. 71, n. 1, p. 103-110, 1996.

KÄLBER, T.; MEIER, J. S.; KREUZER, M.; LEIBER, F. Flowering catch crops used as forage plants for dairy cows: influence on fatty acids and tocopherols in milk. Journal of Dairy Science, Champaign, v. 94, n. 3, p. 1477-1489, 2011.

KEXIAN, Y.; LASCANO, C. E.; KERRIDGE, P. C.; AVILA, P. The effect of three tropical shrub legumes on intake rate and acceptability by small ruminants. Pasturas Tropicales, Cali, v. 20, n. 3, p. 31-35, 1998.

KHIAOSA-ARD, R.; BRYNER, S. F.; SCHEEDER, M. R. L.; WETTSTEIN, H.-R.; LEIBER, F.; KREUZER, M.; SOLIVA, C. R. Evidence for the inhibition of the terminal step of ruminal ?-linolenic acid biohydrogenation by condensed tannins. Journal of Dairy Science, Champaign, v. 92, n. 1, p. 177-188, 2009.

KOBA, K.; YANAGITA, T. Health benefits of conjugated linoleic acid (CLA). Obesity Research & Clinical Practice, Amsterdan, v. 8, n. 6, p. e525-e532, 2014.

LEE, M. R. F. Forage polyphenol oxidase and ruminant livestock nutrition. Frontiers in Plant Science, Lausanne, v. 5, Article 694, p. 1-9, 2014.

LEE, M. R. F.; TWEED, J. K. S.; SCOLLAN, N. D.; SULLIVAN, M. L. Ruminal micro-organisms do not adapt to increase utilization of poly-phenol oxidase protected red clover protein and glycerol-based lipid. Journal of Science and Food Agricultural, Oxford, v. 88, n. 14, p. 2479-2485, 2008.

LEE, Y.-J.; JENKINS, T. C. Biohydrogenation of linolenic acid to stearic acid by the rumen microbial population yields multiple intermediate conjugated diene isomers. Journal of Nutrition, Cambridge, v. 141, n. 8, p. 1445-1450, 2011.

LOURENÇO, M.; RAMOS-MORALES, E.; WALLACE, R. J. The role of microbes in rumen lipolysis and biohydrogenation and their manipulation. Animal, Cambridge, v. 4, n. 7, p. 1008-1023, 2010.

MARTÍNEZ MARÍN, A. L.; GÓMEZ-CORTÉS, P.; GÓMEZ CASTRO, A. G.; JUÁREZ, M.; PÉREZ ALBA, L. M.; PÉREZ HERNÁNDEZ, M.; de la FUENTE, M. A. Animal performance and milk fatty acid profile of dairy goats fed diets with different unsaturated plant oils. Journal of Dairy Science, Champaign, v. 94, n. 11, p. 5359-5368, 2011.

MARTÍNEZ MARÍN, A. L.; GÓMEZ-CORTÉS, P.; GÓMEZ CASTRO, G.; JUÁREZ, M.; PÉREZ ALBA, L.; PÉREZ HERNÁNDEZ, M.; de la FUENTE, M. A. Effects of feeding increasing dietary levels of high oleic or regular sunflower or linseed oil on fatty acid profile of goat milk. Journal of Dairy Science, Champaign, v. 95, n. 4, p. 1942-1955, 2012.

McKAIN, N.; SHINGFIELD, K. J.; WALLACE, R. J. Metabolism of conjugated linoleic acids and 18:1 fatty acids by ruminal bacteria: products and mechanisms. Microbiology, London, v. 156, n. 2, p. 579-588, 2010.

MOSLEY, E. E.; NUDDA, A.; CORATO, A.; ROSSI, E.; JENKINS, T.; McGUIRE, M. A. Differential biohydrogenation and isomerization of [U-13C]Oleic and [1-13C]Oleic acids by mixed ruminal microbes. Lipids, Oxford, v. 41, n. 5, p. 513-517, 2006.

MOURTHÉ, M. H. F.; REIS, R. B.; GAMA, M. A. S.; BARROS, P. A. V.; ANTONIASSI, R.; BIZZO, H. R.; LOPES, F. C. F. Milk fatty acid profile of Holstein x Gyr cows grazing on marandugrass supplemented with increasing levels of roasted soybeans. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 67, n. 8, p. 1150-1158, 2015.

MUI, N. T.; LEDIN, I.; UDÉN, P.; VAN BINH, D. Effect of replacing a rice bran-soya bean concentrate with Jackfruit (Artocarpus heterophyllus) or Flemingia (Flemingia macrophylla) foliage on the performance of growing goats. Livestock Production Science, Amsterdan, v. 72, n. 3, p. 253-262, 2001.

OITICICA, I. N.; FONSECA, C. E. M.; SOUZA, V. C.; SILVA, A. B.; LOPES, F. C. F.; MORENZ, M. J. F. Flemingia macrophylla in goat feeding. Revista Brasileira de Zootecnia, Viçosa, MG, v. 44, n. 9, p. 335-341, 2015.

PATRA, A. K.; SAXENA, J. Dietary phytochemicals as rumen modifiers: a review of the effects on microbial populations. Antonie van Leeuwenhoek, Oxford, v. 96, n. 4, p. 363-375, 2009.

RIBEIRO, C. G. S.; LOPES, F. C. F.; GAMA, M. A. S.; MORENZ, M. J. F.; RODRIGUEZ, N. M. Productive performance and fatty acid composition of milk from dairy cows fed increasing levels of sunflower oil in elephant-grass based diets. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 66, n. 5, p. 1513-1521, 2014.

ROY, N. C.; KNIGHT, T. W.; REYNOLDS, G. W.; DEIGHTON, M. H.; DEATH, A. F.; SINCLAIR, B. R.; PETERS, J. S.; McNABB, W. C. The effect of condensed-tannins in fresh Sulla (Hedysarum coronarium) on the net flux of fatty acids across the mammary gland and their secretion in the milk of lactating ewes. Proceedings of the New Zealand Society of Animal Production, Palmerston North, v. 62, p. 231-235, 2002.

SHINGFIELD, K. J.; BERNARD, L.; LEROUX, C.; CHILLIARD, Y. Role of trans fatty acids in the nutritional regulation of mammary lipogenesis in ruminants. Animal, Cambridge, v. 4, n. 7, p. 1140-1166, 2010.

SHINGFIELD, K. J.; ROUEL, J.; CHILLIARD, Y. Effect of calcium salts of a mixture of conjugated linoleic acids containing trans-10, cis-12 in the diet on milk fat synthesis in goats. British of Journal Nutrition, Cambridge, v. 101, n. 7, p. 1006-1019, 2009.

SILVA, B. C. M.; RODRIGUEZ, N. M.; MORENZ, M. J. F.; GAMA, M. A. S.; MARTINS, C. E.; PACIULLO, D. S. C.; GOMIDE, C. A. M.; ANJOS, A. J.; MADEIRO, A. S.; LOPES, F. C. F. Milk fatty acid profile of Holstein x Gyr cows on 'Marandu' grass pasture under different grazing strategies. Pesquisa Agropecuária Brasileira, Brasília, v. 52, n. 8, p. 652-662, 2017.

SKLAN, D.; ASHKENAZI, R.; BRAUN, A.; DEVORIN, A.; TABOR, K. Fatty acids, calcium soaps of fatty acids, and cottonseeds fed to high yielding cows. Journal of Dairy Science, Champaign, v. 75, n. 9, p. 2463-2472, 1992.

TIEMANN, T. T.; LASCANO, C. E.; WETTSTEIN, H.-R.; MAYER, A. C.; KREUZER, M.; HESS, H. D. Effect of the tropical tannin-rich shrub legumes Calliandra calothyrsus and Flemingia macrophylla on methane emission and nitrogen and energy balance in growing lambs. Animal, Cambridge, v. 2, n. 5, p. 790-799, 2008.

TORAL, P. G.; CHILLIARD, Y.; ROUEL, J.; LESKINEN, H.; SHINGFIELD, K. J.; BERNARD, L. Comparison of the nutritional regulation of milk fat secretion and composition in cows and goats. Journal of Dairy Science, Champaign, v. 98, n. 10, p. 7277-7297, 2015.

TURPEINEN, A. M.; MUTANEN, M.; ARO, A.; SALMINEN, I.; BASU, S.; PALMQUIST, D. L.; GRIINARI, J. M. Bioconversion of vaccenic acid to conjugated linoleic acid in humans. The American Journal of Clinical Nutrition, Rockville, v. 76, n. 3, p. 504-510, 2002.

VASTA, V.; MAKKAR, H. P. S.; MELE, M.; PRIOLO, A. Ruminal biohydrogenation as affected by tannins in vitro. British of Journal Nutrition, Cambridge, v. 102, n. 1, p. 82-92, 2009a.

VASTA, V.; MELE, M.; SERRA, A.; SCERRA, M.; LUCIANO, G.; LANZA, M.; PRIOLO, A. Metabolic fate of fatty acids involved in ruminal biohydrogenation in sheep fed concentrate or herbage with or without tannins. Journal of Animal Science, Champaign, v. 87, n. 8, p. 2674-2684, 2009b.

VASTA, V.; YANEZ-RUIZ, D. R.; MELE, M.; SERRA, A.; LUCIANO, G.; LANZA, M.; BIONDI, L.; PRIOLO, A. Bacterial and protozoal communities and fatty acid profile in the rumen of sheep fed a diet containing added tannins. Applied and Environmental Microbiology, Washington, v. 76, n. 8, p. 2549-2555, 2010.

VLAEMINCK, B.; FIEVEZ, V.; DEMEYER, D.; DEWHURST, R. J. Effect of forage:concentrate ratio on fatty acid composition of rumen bacteria isolated from ruminal and duodenal digesta. Journal of Dairy Science, Champaign, v. 89, n. 7, p. 2668-2678, 2006.

YANG, B.; CHEN, H.; STANTON, C.; ROSS, R. P.; ZHANG, H.; CHEN, Y. Q.; CHEN, W. Review of the roles of conjugated linoleic acid in health and disease. Journal of Functional Foods, Amsterdan, v. 15, p. 314-325, may 2015.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n1p293

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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