Hemoglobin polymorphism in Hampshire Down sheep herd

Fabíola Cristine de Almeida Rego, Flavio Antonio Barca Junior, Luiz Fernando Coelho Cunha Filho, Werner Okano, Luis César da Silva, Agostinho Ludovico, Silvia Manduca Trapp, Elsa Santana, Neide Mariko Tanaka

Abstract


The present study aimed to determine the types of hemoglobin in Hampshire Down crossbreed sheep, and verify that this locus is in Hardy-Weinberg equilibrium. There have been collected 46 blood samples of healthy Hampshire Down crossbreed sheep. These samples were used to separate the hemoglobin per electrophoresis. The electrophoresis of the hemoglobin revealed a slow band characterized as hemoglobin A (HbAA), a fast band characterized as hemoglobin B (HbBB) and two bands in the heterozygous hemoglobin A and B (HbAB). The HbAB type was the most frequently one, followed by hemoglobin A (HbAA) and B (HbBB). The genotypic frequency of individuals BB, AB and AA were 36,95; 54,35 and 8,70% respectively. The allelic frequency of A and B were respectively 35,87% and 64,13%.. The qui square test (?2 = 0.859 and p = 0.6509) confirmed that the tested locus is in Hardy-Weinberg equilibrium.


Keywords


EElectrophoresis; Hemoglobin polymorphism; Sheep.



DOI: http://dx.doi.org/10.5433/1679-0359.2011v32n1p261

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional