Initial shoot development and root architecture of citrus rootstocks

Maria Aparecida da Cruz, Deived Uilian de Carvalho, Denis Santiago da Costa, Ronan Carlos Colombo, Camilla de Andrade Pacheco, Zuleide Hissano Tazima, Carmen Silvia Vieira Janeiro Neves

Abstract


A well-developed root system is a very important characteristic of rootstocks. Initial plant characterization is a potential technique to highlight cultivars with desirable root architecture for the diversification of rootstocks in the national citriculture. Thus, the aim of this study was to evaluate the initial shoot development and root architecture of citrus cultivars used as rootstocks. Seeds from five rootstocks, ‘Cleopatra’ mandarin, ‘Rangpur’ lime, ‘Rough’ lemon, ‘C13’ and ‘Troyer’ citranges, were sown in black polyethylene bags. A completely randomized block design with five replications and one plant per plot was applied. At 30 and 45 days after emergence, shoots and roots were collected for analysis. Shoot analysis measured the total number of leaves, leaf area, stem diameter, plant height, and fresh and dry matter weights. Root analysis evaluated fresh and dry matter weights and root architecture by image analysis, which recorded root traits including primary, secondary, and tertiary root numbers; length and total volume; and the percentage of secondary roots present in the upper third of the primary root. Shoot and root data of fresh and dry weights were submitted to variance analysis and compared using Tukey’s test (5%). The remaining root data were standardized for variance 1 and studied through principal component analysis. The ‘C13’ citrange showed good shoot development, with greater leaf area, plant height, stem diameter, and shoot fresh and dry matter weights during both evaluation periods, followed by the ‘Troyer’ citrange. The ‘Cleopatra’ mandarin had the smallest shoot and root development during both evaluation periods, presenting lower primary root, secondary root, and root volume than other cultivars evaluated at the same time. The ‘Troyer’ citrange had fewer roots in the upper third of the primary root and a higher root insertion angle.

Keywords


Initial plant characterization; Root system; SmartRoot.

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References


ALMEIDA, C. O.; PASSOS, O. S. Citricultura brasileira: em busca de novos rumos desafios e oportunidades na região Nordeste. Cruz das Almas: Embrapa Mandioca e Fruticultura, 2011. 160 p.

ANDRADE, R. A.; MARTINS, A. B. G.; LEMOS, E. G. M.; LUZ, F. J. F.; SILVA, M. T. H. Detecção de polimorfismo em porta-enxertos para citros. Revista Brasileira de Fruticultura, Jaboticabal, v. 29, n. 2, p. 345-349, 2007. DOI: 10.1590/S0100-29452007000200030.

AULER, P. A. M.; FIORI-TUTIDA, A. C. G.; TAZIMA, Z. H. Comportamento da laranjeira ‘Valência’ sobre seis porta-enxertos no noroeste do paraná. Revista Brasileira de Fruticultura, Jaboticabal, v. 30, n. 1, p. 229-234, 2008. DOI: 10.1590/S0100-29452008000100042.

AULER, P. A. M.; LEITE JUNIOR, R. P.; TAZIMA, Z. H.; ANDRADE, P. F. S. A citricultura no Paraná. Citricultura Atual, Cordeirópolis, v. 1, n. 99, p. 17-20, 2014.

BACK, M. M.; ALTMANN, T.; SOUZA, P. V. D. Influence of arbuscular mycorrhizal fungi on the vegetative development of citrus rootstocks. Pesquisa Agropecuária Tropical, Goiânia, v. 46, n. 4, p. 407-412, 2016. DOI: 10.1590/1983-40632016v4642180.

BENJAMIN, G.; TIETEL, Z.; PORAT, R. Effects of rootstock/scion combinations on the flavor of citrus fruit. Journal of Agriculture and Food Chemistry, Washington, v. 61, n. 47, p. 11286-11294, 2013. DOI: 10.1021/jf402892p.

BOVÉ, J. M.; AYRES, A. J. Etiology of three recent diseases of citrus in São Paulo State: sudden death variegated chlorosis and Huanglongbing. IUBMB Life, Oxford, v. 59, n. 4, p. 346-354, 2007. DOI: 10.1080/15216540701299326.

BRASIL. Ministério da Agricultura e da Reforma Agrária. Regras para análise de sementes. Brasília, DF: SNDA/DNDV/CLAV, 2009. 398 p.

DALAL, R. P. S.; THAKUR, A. Fibrous root distribution in pineapple orange trees under semi-arid irrigated ecosystem. Advances in Horticultural Science, Florence, v. 25, n. 1, p. 32-36, 2011. DOI: 10.13128/ahs-12782.

FOOD AGRICULTURAL ORGANIZATION - FAO. Production - crops. Roma: FAO, 2015. Disponível em: http://faostat3.fao.org/compare/E. Acesso em: 02 dez. 2016.

GONÇALVES, S. L.; LYNCH, J. P. Raízes de plantas anuais: tolerância a estresses ambientais, eficiência na absorção de nutrientes e métodos para seleção de genótipos. Londrina: EMBRAPA, 2014. 67 p.

HAUCK, A. L.; NOVAIS, J.; GRIFT, T. E.; BOHN, M. O. Characterization of mature maize (Zea mays L.) root system architecture and complexity in a diverse set of Ex-PVP inbreds and hybrids. Springerplus, Heidelberg, v. 4, p. 424-445, 2015. DOI: 10.1186/s40064-015-1187-0.

LE BOT, J.; SERRA, V.; FABRE, J.; DRAYE, X.; ADAMOWICZ, S.; PAGÈS, L. DART?: a software to analyse root system architecture and development from captured images. Plant and Soil, Netherlands, v. 326, n. 2, p. 261-273, 2010. DOI: 10.1007/s11104-009-0005-2.

LOBET, G.; LOIC, P.; DRAYE, X. A novel image-analysis toolbox enabling quantitative analysis of root system architecture. Plant Physiology, Rockville, v. 157, n. 1, p. 29-39, 2011. DOI: 10.1104/pp.111.179895.

MEDINA, C. L.; RENA, A. B.; SIQUEIRA, D. L.; MACHADO, E. C. Fisiologia dos citros. In: MATTOS JUNIOR, D. M.; NEGRI, J. D.; PIO, R. M.; POMPEU JUNIOR, J. (Ed.). Citros. Campinas: Instituto Agronômico e Fapesp, 2005. p. 39-57.

MOURÃO FILHO, F. A. A.; ESPINOZA-NÚÑEZ, E.; STUCHI, E. S.; ORTEGA, E. M. M. Plant growth, yield, and fruit quality of ‘Fallglo’ and ‘Sunburst’ mandarins on four rootstocks. Scientia Horticulturae, Amsterdam, v. 114, n. 1, p. 45-49, 2007. DOI: 10.1016/j.scienta.2007.05.007.

NEVES, C. S. V. J.; STENZEL, N. M. C.; CARVALHO, S. L. C.; FURLANETO, T. L. R.; OKUMOTO, S. H. Sistema radicular de quatro porta enxertos sob copa de tangerina ‘Poncã’. Ciência e Agrotecnologia, Lavras, v. 32, n. 2, p. 487-492, 2008. DOI: 10.1590/S1413-70542008000200022.

NEVES, C. S. V. J.; TAVARES FILHO, J.; BRITO, O. R.; YAMASHITA, F.; TORMEM, V.; FONSECA, I. C. B. Huerto de cítricos plantado con sistema de cero labranza y sistema convencional. Semina: Ciências Agrárias, Londrina, v. 31, n. 1, p. 1263-1274, 2010. DOI: 10.5433/1679-0359.2010v31n4Sup1p1263.

PILAU, F. G.; ANGELOCCI, L. R. Leaf area and solar radiation interception by orange tree top. Bragantia, Campinas, v. 74, n. 4, p. 476-482, 2015. DOI: 10.1590/1678-4499.0130.

POMPEU JUNIOR, J.; BLUMER, S. Morte súbita dos citros: suscetibilidade de seleções de limão-cravo e uso de interenxertos. Revista Brasileira de Fruticultura, Jaboticabal, v. 30, n. 4, p. 1159-1161, 2008. DOI: 10.1590/S0100-29452008000400052.

POMPEU JUNIOR, J.; BLUMER, S. Híbridos de trifoliata como porta-enxertos para laranjeira Pêra. Pesquisa Agropecuária Tropical, Goiânia, v. 44, n. 1, p. 9-14, 2014. DOI: 10.1590/S1983-40632014000100007.

POMPEU JUNIOR, J. Porta-enxertos. In: MATTOS JUNIOR, D. M.; NEGRI, J. D.; PIO, R. M.; POMPEU JUNIOR, J. (Ed.). Citros. Campinas: Instituto Agronômico e Fapesp, 2005. p. 63-94.

RYAN, P. R.; DELHAIZE, E.; WATT, M.; RICHARDSON, A. E. Plant roots: understanding structure and function in an ocean of complexity. Annals of Botany, Oxford, v. 118, n. 4, p. 555-559, 2016. DOI: 10.1093/aob/mcw192.

SÁNCHEZ-BLANCO, M.; ÁLVAREZ, S.; ORTUÑO, M. F.; RUIZ-SÁNCHEZ, M. C. Root system response to drought and salinity: root distribution and water transport. In: SÁNCHEZ-BLANCO, M.; ÁLVAREZ, S.; ORTUÑO, M. F.; RUIZ-SÁNCHEZ, M. C. Root Engineering: basic and applied concepts. New York: Springer-Verlag, 2014. p 325-352. DOI: 10.1007/978-3-642-54276-3_15.

SARMIENTO, A.I.P; GIULIANI J.C.; SOUZA, P.V.D. Morfologa de frutos e sementes de porta-enxertos de citros cultivados em ambiente protegido. Revista U.D.C.A Actualidad & Divulgación Científica, Cartagena, v. 19, n. 1, p. 17-24, 2016. DOI: 10.31910/rudca.v19.n1.2016.106.

STATISTICAL ANALYSIS SYSTEM - SAS. SAS® University Edition. Cary: SAS Institute Inc., 2014.

STENZEL, N. M. C.; NEVES, C. S. V. J.; GOMES, J. C.; MEDINA, C. C. Performance of ponkan mandarin on seven rootstocks. Hortscience, Alexandria, v. 38, n. 2, p. 176-178, 2003. DOI: 10.21273/HORTSCI.38.2.176.

TAZIMA, Z. H.; NEVES, C. S. V. J.; YADA, I. F. U.; LEITE JÚNIOR, R. P. Performance of ‘Okitsu’ satsuma mandarin trees on different rootstocks in Northwestern Paraná State. Semina: Ciências Agrárias, Londrina, v. 35, n. 5, p. 2297-2308, 2014. DOI: 10.5433/1679-0359.2014v35n5p2297.

ZHU, J.; INGRAM, P. A; BENFEY, P. N.; ELICH, T. From lab to field, new approaches to phenotyping root system architecture. Current Opinion in Plant Biology, London, v. 14, n. 3, p. 310-317, 2011. DOI: 10.1016/j.pbi.2011.03.020.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n4p1393

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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