Imunohistochemistry: detection of microcystin in tilápia exposed to Microcystis aeruginosa (Cyanobacteria) extract

Márcia Kamogae, Elisabete Hiromi Hashimoto, Ana Paula Millet, Aniê Ieda Francabandiera, Camilla Guiotti de Pádua, Ana Paula Frederico Rodrigues Loureiro Bracarense, Maria do Carmo Bittencourt-Oliveira, Ilce Mara de Syllos Colus, Eiko Nakagawa Itano, Ossamu Kawamura, Tomoaki Tsutsumi, Satoshi Nagata, Ken-ichi Harada, Yoshio Ueno, Elisa Yoko Hirooka

Abstract


The deterioration of the water quality due to aquaculture is associated with eutrophication, with bloom of cyanobacteria. Microcystis aeruginosa is distinguished as main producer of microcystins (MCs), group of hepatotoxins with tumor promoter potential. In the present work immunohistochemical method for detection of MC in tilápia (Oreochromis niloticus), fish submitted to intraperitoneal injection (i.p.) or immersion in extract of M. aeruginosa BCCBUSP 262 was developed, using monoclonal antibody anti - MC (M8H5) and polymer peroxidase system. The tilápias (N=42) had been submitted to the seven treatments, three groups inoculated i.p. with 2.0x105, 4.0x105 and 1.0x106 cells. Kg-1 of M. aeruginosa BCCBUSP 262 and four groups exposed to the immersion in different extract concentrations of cyanobacterium. Analyzing liver and muscular tissue for immunohistochemical assay, muscular tissue was not stained. All the animals inoculated i.p. presented positive marking for MC in the liver, but in immersion test, only the ones exposed in the highest dose (1,0x105 cels.mL-1) presented positive marking. Although MC was not detected in muscular tissue, as well as in the liver of animals immersed in extract of M. aeruginosa BCCBUSP 262 in concentrations less than 1.0x105 cels.mL-1, the results would constitute in the base for the methodological development aiming the application of the immunohistochemistry in the rapid diagnosis in quality control of fish.


Keywords


Microcystin; Immunohistochemistry; Fish.



DOI: http://dx.doi.org/10.5433/1679-0359.2007v28n3p427

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional