Interaction between sugarcane families and plant growth-promoting bacteria in two crop cycles

Valéria Rosa Lopes, João Carlos Bespalhok Filho, Guilherme Grodzki Oliveira Figueiredo, Ricardo Augusto de Oliveira, Edelclaiton Daros

Abstract


The response of sugarcane (Saccharum spp.) families to the inoculation with plant growth-promoting bacteria (PGPB) may show positive or negative interaction values depending on the plant genotype, allowing the selection of responsive genotypes to the association. Based on these precepts, this study aimed to evaluate the response of 27 sugarcane families treated with two PGPB-based inoculants in two sugarcane cycles: plant-cane and first ratoon. Four months after germination, 27 families from crosses between clones of sugarcane were: (T0) not inoculated; (T1) inoculated with Triazo, which consists of a mixture of the strains Abv5, Abv6, and Abv7 of Azospirillum brasilense; and (T2) inoculated with an inoculant called Mix, which contains five strains of PGPB (Gluconacetobacter diazotrophicus Pal5, Azospirillum amazonense CBAmC, Burkholderia tropica Ppe8, Herbaspirillum rubrisubalbicans HCc103, and Herbaspirillum seropedicae HRC54). The productivity traits evaluated were the number of stalks, average height, stalk diameter, and Brix. The correlation values between the two cycles were 0.87 for the number of stalks, 0.70 for average height, 0.48 for stalk diameter, and 0.63 for Brix. Differences were observed between treatments for average height, stalk diameter, and Brix. Differences were also observed between families for all the studied traits. Brix was the only parameter that presented a significant interaction between family and inoculant. Different responses were observed between cycles for some families, showing that other factors can also influence the association.

Keywords


PGPB; Sugarcane breeding; Plant-bacteria interaction; Nitrogen-fixing bacteria.

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References


ALCANTARA, R. M. C. M.; ROCHA, M. M.; XAVIER, G. R.; RUMJANEK, N. G. Estado atual da arte quanto à seleção e o melhoramento de genótipos para a otimização da FBN. Teresina. Empresa Brasileira de Pesquisa Agropecuária, EMBRAPA Meio-Norte, 2009. 31 p. (Documento, 196).

ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. The success of BNF in soybean in Brazil. Plant and Soil, The Hague, v. 252, n. 1, p. 1-9, 2003.

ARZANESH, M. H.; ALIKHANI, H. A.; KHAVAZI, K.; RAHIMIAN, H. A.; MIRANSARI, M. In vitro growth of wheat (Triticum aestivum L.) seedlings, inoculated with Azospirillum sp. under drought stress. International Journal of Botany. Cairo, v. 5, n. 3, p. 244-249, 2009.

BALDANI, I. J.; BALDANI, L. V. History on the biological nitrogen fixation research in graminaceous plants: special emphasis on the Brazilian experience. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 77, n. 3, p. 549-579, 2005.

BASHAN, Y.; BASHAN, L. E. How the plant growth-promoting bacterium Azospirillum promotes plant growth: a critical assessment. Advances in Agronomy, London, v. 108, n. 1, p. 77-136, 2011.

BASHAN, Y.; DUBROVSKY, J. G. Azospirillum spp. participation in dry matter partitioning in grasses at the whole plant level. Biology and Fertility of Soils, Firenze, v. 23, n. 4, p. 435-440, 1996.

BASHAN, Y.; HARRISON, K.; WHITMOYER, R. Enhanced growth of wheat and soybean plants inoculated with Azospirillum brasilense is not necessarily due to general enhancement of mineral uptake. Applied and Environmental Microbiology, Washington, v. 56, n. 3, p. 769-775, 1990.

CABALLERO-MELLADO, J.; MARTINEZ-ROMERO, E. Limited genetic diversity in the endophytic sugarcane bacterium Acetobacter diazotrophicus. Applied and Environmental Microbiology, Washington, v. 60, n. 5, p. 1532-1537, 1994.

COELHO, C. H. M.; MEDEIROS, A. F. A.; POLIDORO, J. C.; XAVIER, R. P.; RESENDE, A.; QUESADA, D. M.; ALVES, B. J. R.; BODDEY, R.; URQUIAGA, S. Identification of genotypes of sugar cane with respect to their potential contribution from biological nitrogen fixation. Agronomia, Garça, v. 37, n. 2, p. 37-40, 2003.

DONG, Z.; HEYDRICH, M.; BERNARD, K; MCCULLY, M. E. Further evidence that the N 2 fixing endophytic bacterium from the intercellular spaces of sugarcane stems is Acetobacter diazotrophicus. Applied and Environmental Microbiology, v. 61, n. 5, p. 1843-1846, 1995.

EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA. Sistema Brasileiro de classificação de solos. Brasília: Embrapa Informação Tecnológica, 1999. 412 p.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS - FAO. World reference base for soil resources. Rome: Food and Agriculture Organization, 2006. 145 p.

HARI, K.; SRINIVASAN, T. R. Response of sugarcane varieties to application of nitrogen fixing bacteria under different nitrogen levels. Sugar Tech, Lucknow, v. 7, n. 2-3, p. 28-31, 2005.

HUNGRIA, M.; CAMPO, R. J.; SOUZA, E. M.; PEDROSA, F. O. Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant and Soil, The Hague, v. 331, n. 1-2, p. 413-425, 2010.

INSTITUTO AGRONÔMICO DO PARANÁ - IAPAR. Cartas Climáticas do Estado do Paraná. Londrina: Instituto Agronômico do Paraná, 1994. 49 p.

LOPES, V. R.; BESPALHOK-FILHO, J. C.; ARAUJO, L.; RODRIGUES, F. V.; DAROS, E.; OLIVEIRA, R. A. The selection of sugarcane families that display better associations with plant growth promoting rhizobacteria. Journal of Agronomy, Punjab, v. 11, n. 2, p. 43-52, 2012.

MARIN, V. A.; BALDANI, V. L.; TEIXEIRA, K. R. S.; BALDANI, J. I. Fixação biológica de nitrogênio: bactérias fixadoras de nitrogênio e sua importância para a agricultura tropical. Seropédica: EMBRAPA-CNPAB, 1999. 24 p. Disponível em: http://www.cnpab.embrapa.br/publicacoes/fbn_nao_leg.html. Acesso em: 12 nov. 2010.

MATSUOKA, S.; GARCIA, A. A. F.; ARIZONO, H. Melhoramento da cana-de-açúcar. In: BOREM, A. Melhoramento de espécies cultivadas. Viçosa, MG: Editora UFV, 2005. p. 205-251.

MOREIRA, F. M. S.; SIQUEIRA, J. O. Microbiologia e bioquímica do solo. 2. ed. Viçosa: Editora UFLA, 2006. 729 p.

MOUTIA, J. F. Y.; SAUMTALLY, S.; SPAEPEN, S.; VANDERLEYDEN, J. Plant growth promotion by Azospirillum sp. in sugarcane is influenced by genotype and drought stress. Plant and Soil, The Hague, v. 337, n. 1-2, p. 233-242, 2010.

MUÑOZ-ROJAS, J.; CABALLERO-MELLADO, J. Population dynamics of Gluconacetobacter diazotrophicus in sugarcane cultivars and effect on plant grow. Microbial Ecology, Rockville, v. 45, n. 4, p. 454-464, 2003.

OLIVARES, F. L.; JAMES, E. K.; BALDANI, J. I.; DOBEREINER, J. Infection of mottled stripe disease-susceptible and resistant sugar cane varieties by the endophytic diazotrophyc Herbaspirilium. New Phytologist, Lancaster, v. 135, n. 4, p. 723-737, 1997.

OLIVEIRA, A. L. M.; CANUTO, E. L.; URQUIAGA, S.; REIS, V. M.; BALDANI, J. I. Yield of micropropagated sugarcane varieties in different soil types following inoculation with diazotrophic bacteria. Plant and Soil, The Hague, v. 284, n. 1-2, p. 23-32, 2006.

OLIVEIRA, A. L. M.; STOFFELS, M.; SCHMID, M.; REIS, V. M.; BALDANI, J. I.; HARTMANN, A. Colonization of sugarcane plantlets by Mixed inoculations with diazotrophic bacteria. European Journal of Soil Biology, Braunschweig, v. 4, n. 5, p. 106-113, 2009.

PEDROZO, C. A.; BARBOSA, M. H. P.; SILVA, F. L.; RESENDE, M. D. V.; PETERNELLI, L. A. Repeatability of full-sib sugarcane families across harvests and the efficiency of early selection. Euphytica, Dordrecht, v. 182, n. 3, p. 423-430, 2011.

PEREIRA, W. Produtividade e qualidade tecnológica da cana-de-açúcar inoculada com bactérias diazotróficas. 2011. Dissertação (Mestrado em Agronomia) - Universidade Federal Rural do Rio de Janeiro, Seropédica.

PÉREZ, J.; CASAS, M. Efecto de la interacción planta-Azospirillum en el cultivo de la caña de azúcar (Saccharum sp). Cultivos Tropicales, La Habana, v. 26, n. 4, p.13-19, 2005.

RESENDE, M. D. V. de. O software selegen-Reml/Blup. Campo Grande: EMBRAPA Informação Tecnológica, 2006. 67 p.

ROESCH, L. F. W.; PASSAGLIA, L. M. P.; BENTO, F. M.; TRIPLETT, E. W.; CAMARGO, F. A. O. Diversidade de bactérias diazotróficas endofíticas associadas a plantas de milho. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 31, n. 6, p. 1367-1380, 2007.

TEJERA, N.; LLUCH, C.; MARTÍNEZ-TOLEDO, M. V.; GONZÁLEZ-LOPEZ, G. Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere. Plant and Soil, The Hague, v. 270, n. 1, p. 223-232, 2005.

TORTORA, M. L.; DIAZ-RICCI, J. C.; PEDRAZA, R. O. Azospirillum brasilense siderophores with antifungal activity against Colletotrichum acutatum. Archives of Microbiology, Braunschweig, v. 193, n. 4, p. 275-286, 2011.

URQUIAGA, S.; CRUZ, K. S.; BODDEY, R. M. Contribution of nitrogen fixation to sugar cane: Nitrogen-15 and nitrogen-balance estimates. Soil Science Society of America Journal, Madison, v. 56, n. 1, p. 105-114, 1992.

VERMA, J. P.; YADAV, J.; TIWARI, K. N.; LAVAKUSH SINGH, V. Impact of plant growth promoting rhizobacteria on crop production. International Journal of Agricultural Research, v. 5, n. 11, p. 954-983, 2010.




DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n2p527

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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