Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits

Saulo Tadeu Lemos Pinto Filho, Matheus Pippi da Rosa, Tiago Luis Eilers Treichel, Fabíola Dalmolin, Alencar Kolinski Machado, Francine Carla Cadoná, Ivana Beatrice Mânica da Cruz

Abstract


Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4.

Keywords


Cell therapy; MSC; Oryctolagus cuniculus; ROS.

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DOI: http://dx.doi.org/10.5433/1679-0359.2018v39n5p1993

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional