Utilization of raw materials from agroindustry – sugar cane juice and yeast extract – for asparaginase production by Zymomonas mobilis CP4

Josiani Fatima Casotti, Doumit Camilios Neto, Gilcelene Bruzon, Robson Alessandro Mattos Machado, João Batista Buzato, Maria Antonia Pedrine Colabone Celligoi

Abstract


Sugar cane juice and yeast extract have been used for asparaginase production by Z. mobilis CP4. A complete factorial design of two variables (yeast extract and asparagin) at three levels (1.0; 5.5 and 10.0 g/L) with one replication at the central point was used. Batch fermentation utilised sugar cane juice diluted at 8 % (W/V) of Total Sugars and an inoculum of 2 mg of cells/mL. After fermentation time of 18 hours, the highest production of asparaginase was 9.75 U/L using both yeast extract (5.5 g/L) and asparagin (1.0 g/l).

Keywords


Sugar cane juice; Zymomonas mobilis; Asparaginase; Fermentation.



DOI: http://dx.doi.org/10.5433/1679-0359.2007v28n4p653

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional