Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria

Valéria Carpentieri-Pípolo, Maria Gallo-Meagher, Don W. Dickson, Daniel W. Gorbet, Maria de Lurdes Mendes, Silvia Graciele Hülse de Souza

Abstract


This  work  had  the  aim  of  comparing  the  method  of  hybridization  with  digoxigenin  and the chemiluminescent detection  system with  the method of  radioactive  labeled. The cultivars used were susceptible Florunner  and  resistant COAN. The genomic DNA was  isolated  from young  leaves  and Southern blotting analysis was conducted using RFLP probe R2430E. The detection of the probe labeled with digoxigenin was as sensitive as labeled by the radioactivity, however with advantages above the method  radioactive. Due  to be a  safe method and  to present  reduced costs,  the demarcation method based on the digoxigenin and chemiluminescent detection is highly recommendable.


Keywords


Nonradiactive detection; Digoxigenin; Molecular markers.



DOI: http://dx.doi.org/10.5433/1679-0359.2008v29n4p783

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional