Fungitoxicty of Bidens pilosa, Thymus vulgaris, Lippia alba and Rosmarinus officinalis in the in vitro development of phytophatogens fungi

Oscar Koutaro Tagami, Marcos Diamantino G. Gasparin, Kátia Regina Freitas Schwan-Estrada, Maria Eugênia da Silva Cruz, Adriana Terumi Itako, João Batista Tolentino Júnior, Luciano M. de Moraes, José Renato Stangarlin

Abstract


The fungitoxicity of aqueous crude extracts (ACE) of Bidens pilosa, Thymus vulgaris, Lippia alba and Rosmarinus officinalis was evaluated in vitro on development of Alternaria alternata, Colletotrichum graminicola, Rhizoctonia solani and Sclerotium rolfsii. The active ACE were incorporated to Potato- Dextrose-Agar (PDA) alone or in mixture, at 0%, 1%, 5%, 10%, 15%, 25% and 50% concentrations. Measurement in fungal colonies was taken daily until the control treatment covered 2/3 of the culture media. The mixtures of T. vulgaris with B. pilosa did not have positive effect because the ACE of B. pilosa did not reduce the fungal growth of R. solani and in mixture inhibited the effect ACE of T. vulgaris. However, the mixture of ACEs of R. officinalis and L. alba produced better results than isolated ACEs, with 60% of growth inhibition of A. alternata. The ACE of T. vulgaris inhibited the growth of C. graminicola at 97%. The results show that ACEs have significative fungitoxicity in the evaluated phythopathogens.


Keywords


Medicinal plants; Alternaria alternata; Colletotrichum graminicola; Rhizoctonia solani; Sclerotium rolfsii.



DOI: http://dx.doi.org/10.5433/1679-0359.2009v30n2p285

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional