Parasitological analysis of green leaf lettuce cultivated in different production systems

Juliana Santiago Santos, Cristina Atsumi Kuba, Francislaine Anelize Garcia Santos, Aline da Silveira Batista, Stênio Clemente Paião Sitolino, Ana Caroliny Carrion Pereira, Rogério Giuffrida, Vamilton Alvares Santarém

Abstract


This study analysed parasite contamination in green leaf lettuce (Lactuca sativa), grown in different cultivation systems (conventional, organic, and hydroponic), from a family farmer cooperative in the municipality of Presidente Prudente, São Paulo, Brazil. Samples were collected at weekly intervals during five months, totalling 180 vegetable samples (60 samples of leaf lettuce from each cultivation system). Lettuce leaves were washed with 0.5% Extran MA 02, and the resulting fluid subjected to sedimentation and centrifugal flotation for recovery of parasite structures. Overall, 71 samples (39.4%) were contaminated with at least one parasite structure, 34 (47.9%) from lettuce grown in the hydroponic system, 20 (28.2%) from the organic system, and 17 (23.9%) from the conventional system. Entamoeba spp. cysts were the most common parasite structures found in the leafy vegetables, with the highest cyst counts found in the hydroponic system (p = 0.003). It is concluded that, regardless of the cultivation system (conventional, organic, or hydroponic), there is a possibility of green leaf lettuce contamination by intestinal parasites. Measures that improve sanitary conditions during production, as well as proper hygiene during the preparation of raw leafy vegetables, may be important to reduce contamination and consequent transmission of parasite diseases from raw leafy vegetable consumption.

Keywords


Enteroparasites; Cultivation systems; Leafy vegetables; Zoonoses.

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DOI: http://dx.doi.org/10.5433/1679-0359.2017v38n2p801

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional