Phytochemical investigation of dichloromethanic fraction on Ocimum gratissimum L. extract

Michele Rosset, Vitor de Moraes Zamarion, Milton Faccione, Terezinha de Jesus Faria, Jurandir Pereira Pinto, Aneli de Melo Barbosa, José Roberto Pinto de Souza

Abstract


Previous studies showed that ethanolic extract of Ocimum gratissimum inhibited growth of Penicillium chrysogenum, Rhizoctonia sp, Aspergillus niger and Alternaria sp, isolated from tomato and carrot. The aim of this study was to identify the chemical constituents of Ocimum gratissim. Aerial parts of the plant were treated with hexane-dichloromethane-ethanol mixture (1:3:1) at room temperature. The obtained extract was chromatographed on silica gel column eluted with hexane, dichloromethane and ethanol. Fractions eluted with dichloromethane were rechromatographed on a column of silica gel to yield alyphatic hydrocarbon mixture (C31, C33, C34 e C35), eugenol, caryophyllene oxide and a mixture of stigmasterol and sitosterol. The structures of the compounds were deduced by spectral data analysis and by comparison with literature values. Recent studies showed that eugenol is probably one of the responsibles for antifungal activity of O. gratissimum.


Keywords


Ocimum gratissimum; Antifungal activity; Eugenol; Phytopathogenic fungi.



DOI: http://dx.doi.org/10.5433/1679-0359.2005v26n4p515

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
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