Evaluation of semen parameters of boars (Sus scrofa) experimentally infected with Toxoplasma gondii

Anderson Barbosa de Moura, Sérgio Jordão Filho, Daniela Cristina Di Mauro, Beatriz Bassora Paim, Fernanda de Rezende Pinto, Alvimar José da Costa

Abstract


Aiming to investigate the influence of T. gondii on semen parameters and spermatozoa morphology, eight boars were inoculated with T. gondii. Experimental groups consisted of: GI (n=3) 1,5 x 104 oocysts of P strain; GII (n=3) 1,0 x 106 tachyzoites of RH strain and GIII (n=2), control noninoculated. Evaluations of semen parameters (volume, motility, strength, concentration), study of spermatozoa morphology, serology (RIFI), parasitemia and hemograms were performed. For this purpose, blood and semen collection were carried out on days -2, -1, 1, 3, 5, 7, 9, 11, 14 and weekly until 84 days post-inoculation. Non hematimetrics alterations and clinical signs were observed on animals. Parasitemia was detected in an animal inoculated with oocysts, on the 7th day post inoculation (DPI) and in a two pigs of GII (tachyzoites), on the 3rd and 49th DPI. Serology results revealed the presence of antibody anti-T. gondii on the animals inoculated with oocysts or tachyzoites since 7th DPI, with tittles of 256 and 64, reaching a maximal level of 4096 on days 11 and 9 post inoculation, respectively. The GIII (control) was negative through out all experimental period. The semen parameters evaluated did not present any alteration due to toxoplasmosis. Significative differences (P < 0,05), considering spermatozoa pathology, were not observed between inoculated and control groups.


Keywords


Toxoplasmosis; Boar; Semen parameters; Spermatozoa morphology.



DOI: http://dx.doi.org/10.5433/1679-0359.2004v25n2p107

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional