Ocorrence of Neospora caninum antibody in dairy cows assayed by ELISA and IFAT from Avaré county, SP

Izidoro Francisco Sartor, Marcia Yumiko Hasegawa, Aurea Maria Oliveira Canavessi, Rhonda Denise Pinckney

Abstract


Sera of 521 dairy cows from herds in Avaré County, SP were examined by indirect fluorescent-antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Neospora caninum. The positive samples for Neospora were also tested for Toxoplasma gondii by IFAT. By using IFAT 15.9% of the samples reacted for N. caninum with dilution between 1:200 and 1: 1600. By using ELISA 30.5% were seropositive for Neospora. Of the samples that were positive to ELISA and negative to IFAT for antibodies titers of 1:200, 46 showed IFAT titers of 1:160. All 129 cows that were seropositive to IFAT with titers of 1:200 and 1:160 were also positive to ELISA. Out of 44 detected abortions, 14 (31.8%) and 18 (40.9%) cows had IFAT titers of ³ 1:200 and ³ 1:160, respectively, and 24 (54.5%) cows were positive to ELISA. Of the 159 seropositive samples to N. caninum to IFAT and/or ELISA, only six were positive to IFAT to T. gondii. Moreover, among the 44 cows with history of abortion, only one demonstrated antibodies to Toxoplasma. These results suggest the presence of N. caninum in the herds that were evaluated and the potential relationship with the observed abortions.


Keywords


Neosporosis; Bovine; Apicomplexa.



DOI: http://dx.doi.org/10.5433/1679-0359.2003v24n1p3

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional