Antimicrobial susceptibility of Clostridium perfringens isolated from domestic and wild animal species in Brazil

Carlos Augusto de Oliveira Júnior, Rodrigo Otávio Silveira Silva, Amanda Nadia Diniz, Prhiscylla Sadanã Pires, Felipe Masiero Salvarani, Ronnie Antunes de Assis, Francisco Carlos Faria Lobato

Abstract


Clostridium perfringens is a microorganism commonly found in the microbiota of humans and animals and a potential cause of enteric, muscle or nervous diseases. The treatment of these diseases is based on antimicrobial therapy and it is extremely important to know the antimicrobial susceptibility profile of the strains present in the region. The aim of this study was to evaluate the antimicrobial susceptibility of C. perfringens isolated from domestic and wild animals in Brazil against seven different antimicrobials. Forty-one strains from the stool samples of cattle (n = 12), buffalo (n = 2), goat (n = 3), dogs (n = 12) and wild carnivores (n = 12) were examined. The minimum inhibitory concentration was determined by the agar dilution method using Brucella agar supplemented with 5% of sheep blood, 0.1% of vitamin K, 0.1% of hemin and concentrations ranging from 0,25 to 256,0 mg L-1 of the following antibiotics: erythromycin, florfenicol, metronidazole, oxytetracycline, penicillin, tylosin, and vancomycin. All C. perfringens strains were susceptible to florfenicol, metronidazole, penicillin and vancomycin. Two strains (4.9%) were resistant to erythromycin and tylosin, while five (12.2%) were resistant to oxytetracycline, one of which (2.4%) from an ocelot.


Keywords


Resistance; Sensibility antibiotics; Buffalo; Cattle; Dogs; Carnivores.

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DOI: http://dx.doi.org/10.5433/1679-0359.2016v37n1p257

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional