Imunoidentification of Albumin and Osteopontin in Seminal Plasma of Taurine and Zebuine Bulls

Eneder Rosana Oberst, Maria Inês M. Jobim, Helena Iturvides Cimarosti, Diogo Onofre Souza, Christianne Gazzana Salbego, Vera Beatriz Wald, Rodrigo Costa Mattos

Abstract


Two dimensional polyacrylamide gel electrophoresis was performed in seminal plasma of seven Bos taurus taurus and seven Bos taurus indicus bulls with high semen freezability, from an artificial insemination center. In a 8% polyacrylamide gels, three bands of 195, 66 and 55 kDa, present in 100% of the samples in both sub-species, were analyzed by their optical densities. In Bos taurus samples, the opticals densities of 55 kDa band, imunoidentified as osteopontin were superior (p < 0.05) than Bos indicus samples. The 66 kDa band was imunoidentified as albumin. In the Bos taurus samples, the bands did not show any variation among the bull samples, but in the Bos indicus, the albumin optical densities showed significant variation among samples.


Keywords


Bovines; Seminal Plasma; Proteins ; Bidimensional electrophoresis.



DOI: http://dx.doi.org/10.5433/1679-0359.2002v23n1p21

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional