Humoral immune response of horses experimentally infected with Trypanosoma evansi

Luiz Carlos Marques, Rosângela Zacarias Machado, Antônio Carlos Alessi, Lúcia Padilha Cury Thomaz de Aquino

Abstract


Six adult horses were experimentally infected with Trypanosoma evansi (106 parasites). Three other adult horses served as negative control. Serum samples of the experimentally infected horses with T. evansi and non-infected controls horses were obtained before inoculation, and daily thereafter until 14 days post infection (DPI). After that time the serum samples were obtained weekly. Sera of the infected and non-infected control horses was tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. evansi. Both ELISA and IFAT detected trypanosomal antibodies shortly after infection and showed progressive increases in antibodies levels during early stages of infection. The responses started on the eighth and eleventh DPI. Maximum IFAT and ELISA values were reached after four weeks of infection and were maintained at this level until the end of the period of study.


Keywords


Trypanosoma evansi; Trypanosomiasis; Horses; Immune response.



DOI: http://dx.doi.org/10.5433/1679-0359.2001v22n2p131

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional