Effects of exposure to halothane, isoflurane, and sevoflurane on embryo viability and gestation in female mice

Autores

  • Ademir Cassiano Rosa Faculdade de Medicina de Botucatu, Universidade Estadual Paulista
  • Suzane Lilian Beier Universidade Federal de Minas Gerais
  • Nilson Oleskovicz Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias
  • Joana Cláudia Mezzalira Universidade do Estado de Santa Catarina
  • Davi José Miquelutti Universidade do Estado de Santa Catarina
  • Alceu Mezzalira Universidade do Estado de Santa Catarina
  • Aury Nunes de Moares Universidade do Estado de Santa Catarina

DOI:

https://doi.org/10.5433/1679-0359.2015v36n2p871

Palavras-chave:

Embryo, Foetus, Gestation, Litter size, Inhalation anaesthetic.

Resumo

Although the negative effects of inhalation anaesthetics on fertility have been known for a while, the stages during the reproductive cycle at which these effects occur and the mechanisms of action are largely unknown. This study aimed to evaluate the effects of acute exposure of female mice to halothane, isoflurane, and sevoflurane prior to mating. BALB/c female mice (n=160) were allocated in groups of 20 to halothane (HG), isoflurane (IG), sevoflurane (SG), and oxygen/sham (SH) treatment groups and their respective control groups (CGs). The mice were exposed to 1 minimum alveolar concentration (MAC) of the corresponding anaesthetic or oxygen for 4 h/day over 5 consecutive days. Two days after exposure, females were mated with males (ratio 2:1 female/male) for five consecutive days. Every morning, females were checked for the presence of vaginal plugs. Half of the females that exhibited plugs were euthanised 72 h later for embryo evaluation. The remaining females were euthanised on the 14th day of pregnancy for foetal evaluation. A low number of morulae and total embryos (morulae + blastocysts) were observed in the HG (P<0.05). The number of implantations was lower in the HG (6.0) compared with the IG (11.8) and SG (12.4) (P<0.05). Exposure to halothane is not recommended for use in female mice prior to mating procedures because it leads to decreased embryo production and pregnancy failure. Sevoflurane induces a delay in embryo development, thereby influencing the morulae to blastocyst ratio. Isoflurane is the safest of the tested anaesthetics for use in female mice prior to mating.

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Biografia do Autor

Ademir Cassiano Rosa, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista

Prof. Dr. of Department of Veterinary Medicine, School of Agriculture and Veterinary Science, Santa Catarina State University, CAV/UDESC, Lages, SC, Brazil.

Suzane Lilian Beier, Universidade Federal de Minas Gerais

Profa Dra of Department of Clinical and Veterinary Surgery, School of Veterinary, Federal University of Minas Gerais, UFMG, Belo Horizonte, MG, Brazil.

Nilson Oleskovicz, Universidade do Estado de Santa Catarina, Centro de Ciências Agroveterinárias

Prof. Dr. of Department of Veterinary Medicine, School of Agriculture and Veterinary Science, Santa Catarina State University, CAV/UDESC, Lages, SC, Brazil.

Joana Cláudia Mezzalira, Universidade do Estado de Santa Catarina

Doctoral student of Department of Veterinary Medicine, School of Agriculture and Veterinary Science, Santa Catarina State University, CAV/UDESC, Lages, SC, Brazil.

Davi José Miquelutti, Universidade do Estado de Santa Catarina

Prof. Dr. of Department of Land and Natural Resources, School of Agriculture and Veterinary Science, Santa Catarina State University, CAV/UDESC, Lages, SC, Brazil.

Alceu Mezzalira, Universidade do Estado de Santa Catarina

Prof. Dr. of Department of Veterinary Medicine, School of Agriculture and Veterinary Science, Santa Catarina State University, CAV/UDESC, Lages, SC, Brazil.

Aury Nunes de Moares, Universidade do Estado de Santa Catarina

Prof. Dr. of Department of Veterinary Medicine, School of Agriculture and Veterinary Science, Santa Catarina State University, CAV/UDESC, Lages, SC, Brazil.

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Publicado

2015-04-22

Como Citar

Rosa, A. C., Beier, S. L., Oleskovicz, N., Mezzalira, J. C., Miquelutti, D. J., Mezzalira, A., & Moares, A. N. de. (2015). Effects of exposure to halothane, isoflurane, and sevoflurane on embryo viability and gestation in female mice. Semina: Ciências Agrárias, 36(2), 871–882. https://doi.org/10.5433/1679-0359.2015v36n2p871

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