Monoclonal and polyclonal antibodies for ante- and post-mortem detection of PrPSc in sheep

Autores

  • Helen Caroline Raksa Universidade Católica do Paraná
  • Michele Dietrich Moura Costa Universidade Estadual de Ponta Grossa
  • Lorenzo González Veterinary Laboratories Agency, Pentlands Science Park
  • Caroline Pinto de Andrade Universidade Federal do Rio Grande do Sul
  • David Driemeier Universidade Federal do Rio Grande do Sul
  • Jair Rodini Engracia Filho Universidade Católica do Paraná
  • Cristina Santos Sotomaior Universidade Católica do Paraná

DOI:

https://doi.org/10.5433/1679-0359.2015v36n2p849

Palavras-chave:

Scrapie, Diagnosis, Immunohistochemistry, Antibody.

Resumo

Scrapie is a disease that affects sheep and goats and is characterized by the accumulation of an abnormal isoform (PrPSc) of the cellular prion protein, PrPC, in the central nervous system (CNS) and in lymphoid tissues. Detection of PrPSc in these tissues can be attempted by a variety of techniques, including immunohistochemistry (IHC) and western blotting (WB), for which a wide range of monoclonal and polyclonal antibodies are commercially available. The objective of this study was to test and compare the efficacy of monoclonal antibodiesF89/160.1.5, F99/97.6.1, and P4 and polyclonal antibodies M52 and R486 in the detection of PrPSc in lymphoid and CNS tissue samples by using IHC. Positive and negative control samples of sheep brain and tonsils were provided by the Animal Health and Veterinary Laboratories Agency (AHVLA, UK). The IHC examination of CNS samples with both monoclonal and polyclonal antibodies confirmed the granular deposition of PrPSc in the neurons of the positive control tissues. However, while the monoclonal antibodies did not produce positive reactions in the negative controls, the polyclonal antibodies showed some non-specific staining. The testing of positive control tonsil samples with polyclonal and monoclonal antibodies identified positive control-specific reactions, whereas the negative control tissues were IHC-negative with all antibodies, although P4 and the polyclonal antibodies produced some background staining. In summary, although the polyclonal antibodies may be more accessible, their use is not advisable because of possible false positive reactions. The polyclonal antibody M52 was able to identify PrPC in brain and spleen samples by WB but other lymphoid tissues were negative.

Métricas

Carregando Métricas ...

Biografia do Autor

Helen Caroline Raksa, Universidade Católica do Paraná

Discente do Programa de Pós-Graduação em Ciência Animal, Escola de Ciências Agrárias e Medicina Veterinária, Pontifícia Universidade Católica do Paraná, PUCPR, São José dos Pinhais, PR, Brasil.

Michele Dietrich Moura Costa, Universidade Estadual de Ponta Grossa

Profª, Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, PR, Brasil.

Lorenzo González, Veterinary Laboratories Agency, Pentlands Science Park

Veterinary Research Pathologist, Animal Health and Veterinary Laboratories Agency, AHVLA, Pentlands Science Park, Bush Loan, PENICUIK, Midlothian, Scotland, UK.

Caroline Pinto de Andrade, Universidade Federal do Rio Grande do Sul

Discente de Pós-Doutoranda do Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre, RS, Brasil.

David Driemeier, Universidade Federal do Rio Grande do Sul

Prof., Programa de Pós-Graduação em Ciências Veterinária, UFRGS, Porto Alegre, RS, Brasil.

Jair Rodini Engracia Filho, Universidade Católica do Paraná

Prof., Programa de Pós-Graduação em Ciência Animal, Escola de Ciências Agrárias e Medicina Veterinária – Pontifícia Universidade Católica do Paraná, PUCPR, São José dos Pinhais, PR, Brasil.

Cristina Santos Sotomaior, Universidade Católica do Paraná

Profª, Programa de Pós-Graduação em Ciência Animal, Escola de Ciências Agrárias e Medicina Veterinária – Pontifícia Universidade Católica do Paraná, PUCPR, São José dos Pinhais, PR, Brasil.

Downloads

Publicado

2015-04-22

Como Citar

Raksa, H. C., Costa, M. D. M., González, L., Andrade, C. P. de, Driemeier, D., Engracia Filho, J. R., & Sotomaior, C. S. (2015). Monoclonal and polyclonal antibodies for ante- and post-mortem detection of PrPSc in sheep. Semina: Ciências Agrárias, 36(2), 849–862. https://doi.org/10.5433/1679-0359.2015v36n2p849

Edição

Seção

Artigos

Artigos mais lidos pelo mesmo(s) autor(es)

1 2 > >>