Hematology and time of macrophage migration catfish Pseudoplatystoma spp. front of inoculation Saccharomyces cerevisiae

André Luiz Nunes, Robson Andrade Rodrigues, Leticia Emiliani Fantini, Nandara Soares de Oliveira, Pamela Thainara do Nascimento Veiga, Rodrigo Yutaka Dichoff Kasai, Cristiane Meldau de Campos

Abstract


It was studied in this work the definition of time in phagocytic activity of macrophages in the celomatic cavity in surubim Pseudoplatystoma spp. and the hematological and biochemistry alterations after the inoculation of Saccharomyces cerevisae. 24 fishes weighting an average of 15,6 ± 4,3 g were used. The delineation used was entirely randomized, and the incubation times were one, two and three hours, and a control group, where six fishes were used per group. For inoculation, 1.5 mL of solution of yeast Saccharomyces cerevisiae in concentration of 9,000 cells mm-3 were injected intraperitoneally. The hematological and biochemistry variables analyzed were: hematocrit, hemoglobin, total number of erythrocytes, mean corpuscular volume, mean corpuscular hemoglobin concentration, trombocyte count of total blood and total count of differential leukocyte and glucose. The phagocytic capacity and index of macrophages were evaluated. Variance analysis was performed (P<0.05) and the means that showed significative diffence were compared through the Tukey test at 5%. The eosinophills concentration was lower in the times of two and three hours compared to the control group. There was not significative difference to the plasmatic glucose among the times of incubation. The incubation period of two hours proved to be enough to promote migration and optimal activation of serubim macrophages.


Keywords


Aquaculture; Phagocytosis; Hematology; Immunity; Yeast; Fish.



DOI: http://dx.doi.org/10.5433/1679-0359.2014v35n2p867

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional