Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC

Alais Maria Dall Agnol, Marielly Bastos Cavalcante, Chirles Araújo de França, Carina da Costa Krewer, Anderson Antônio de Queiros, Mateus Matiuzzi da Costa, José Francisco Manta Bragança, Lilian Kolling Girardini

Abstract


This study aimed to determine the antimicrobial susceptibility profile and to assess the presence of mechanisms of antimicrobial resistance in Staphylococcus spp. (n=36) isolated from mastitis in sheep’s Chapecó-SC. The potential for biofilm production was determined by phenotypic tests of Congo Red Agar, DAPI and Gentian Violet and by PCR for the detection of icaD gene. To evaluate the antimicrobial resistance testing was performed disk diffusion and detection of resistance genes blaZ, mecA, ermA, ermB and ermC and msrA also performed by PCR.The pump test was conducted by efuxo crop growth Muller Hinton agar containing ethidium bromide. The results showed that 1 (2,78%), 36 (100%) and 10 (27,78%) isolates were considered to produce a biofilm on Congo Red Agar test, Gentian Violet and DAPI, respectively, while the gene icaD was observed in only 2 (5.55%) isolates. The lowest percentage of sensitivity was observed for ampicillin (58.33%) and penicillin (58.33%). All strains tested were negative for the mecA, ermA, ermB and ermC genes. However, the isolates were positive for other resistance genes, being the blaZ and the msrA, with percentages of positivity of 58.33% and 11.11% respectively. Only one sample was positive for efflux pump test.


Keywords


Mastitis; sheep; Resistance genes; Efflux pump; Biofilm; PCR.



DOI: http://dx.doi.org/10.5433/1679-0359.2013v34n1p311

Semina: Ciênc. Agrár.
Londrina - PR
E-ISSN 1679-0359
DOI: 10.5433/1679-0359
E-mail: semina.agrarias@uel.br
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional