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Anatomy Of A Crumbling Marriage - Marriage

por Demi Low (2020-05-25)


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A method of testing or determining in vivo acute neurotoxicity of a molecule comprising measuring calcium oscillations in vitro in neuronal cells which are in contact with the molecule. 4. The method of claim 3, wherein the calcium oscillations in the neuronal cells that have been in contact with the molecule are about 70% or higher, about 75% or higher, about 80% or higher, about 85% or higher, about 90% or higher, about 95% or higher, about 96% or higher, about 97% or higher, about 98% or higher, about 99% or higher, about 100% or higher, about 120% or higher, about 140% or higher, about 160% or higher, about 180% or higher, about 200% or higher, about 220% or higher, about 240% or higher, or about 250% or higher compared to the calcium oscillations in the vehicle control cells. 2. The method of claim 1, wherein the calcium oscillations in the neuronal cells that are in contact with the molecule are about 70% or higher, about 75% or higher, about 80% or higher, about 85% or higher, about 90% or higher, about 95% or higher, about 96% or higher, about 97% or higher, about 98% or higher, about 99% or higher, about 100% or higher, about 120% or higher, about 140% or higher, about 160% or higher, about 180% or higher, about 200% or gold show cam higher, about 220% or higher, about 240% or higher, asian nude model or about 250% or higher compared to the calcium oscillations in vehicle control cells.



3. A method of selecting or identifying a molecule having tolerable in vivo acute neurotoxicity comprising measuring calcium oscillations in vitro in neuronal cells which are in contact with the molecule, wherein the neuronal cells in contact with the molecule exhibit calcium oscillations at a level comparable to or higher than that of vehicle control cells. 9. A method of determining in vivo acute neurotoxicity of a molecule comprising a polynucleotide, the method comprising calculating a sequence score, wherein the sequence score is calculated by formula (I): (number of C nucleotides or analogs thereof in the polynucleotide-number of G nucleotides or analogs thereof in the polynucleotide)/total nucleotide length (number) of the polynucleotide (I). 8. The method of claim 1, wherein the molecule comprises a polynucleotide, the method further comprising calculating a sequence score, wherein the sequence score is calculated by formula (I): (number of C nucleotides or analogs thereof in the polynucleotide-number of G nucleotides or analogs thereof in the polynucleotide)/total nucleotide length (number) of the polynucleotide (I).